The exposure was performed with the standard Smith–Robinson approach to the anterior spine [16 (link)]. Decompression of the levels was performed, and cages were placed in the disc spaces. The spine was stabilized by locked plates (Gradient Plus Anterior Cervical Plate systems; NuVasive, San Diego, CA, USA). After irrigation of the wound, a piece of Gelfoam sponge (Pfizer), cut to span the full length of the operated levels in each patient, was soaked with a steroid solution containing 40 mg Depo-Medrol and placed adjacent to the operated levels before closure. The wound was closed over a Penrose drain that was removed on postoperative day 1 or 2 in all patients.
Gelfoam sponge
Manufactured by Pfizer
Sourced in United States
Gelfoam sponge is a sterile, absorbable gelatin sponge made from purified porcine skin gelatin. It is designed to be used as a hemostatic agent to control bleeding in surgical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
2 mercaptoethanol, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Cloning cylinder, by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions)
Isopore membrane filter, by Merck Group (1 mentions)
7 protocols using gelfoam sponge
1
Anterior Cervical Decompression and Fusion
2
Seeded Thymus Lobe Culture Protocol
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Seeded thymus lobes were cultured on 0.8 μm isopore membrane filters (Millipore) supported by a Gelfoam sponge (Pfizer) at an interface between 5% CO2-humidified air and RPMI 1640-based culture medium containing 10% FCS, 100 U/ml penicillin and 100 μg/ml streptomycin (PAA), 2 mM L-glutamine (PAA), 1X non-essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), 10 mM HEPES, 50 μM 2-mercaptoethanol (Sigma). Details have been described (Ueno et al., 2005 (link)).
3
HIV Infection Modeling of Foreskin and Urethra
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Inner foreskin and penile urethra explants were infected with HIVBal as previously described21 (link). Briefly, small scalpel cuts were punctured into the mucosal surface of the tissue before cloning cylinders (8 × 8 mm, Sigma-Aldrich) were adhered to the tissue using surgical glue (B Braun, Germany). Explants were cut around the cloning cylinder and placed onto gel foam sponge (Pfizer, New York, NY, USA) soaking in DC culture media in a 24-well plate. HIV was diluted in 100 µl of PBS at a TCID50 of 3500 and then added to cloning cylinders. In all, 100 µl of PBS was added to mock samples to prevent tissue drying out. Explants were cultured for 2–3 h before virus/PBS was removed and cylinders washed out 3x with PBS. After removal of cloning cylinders, tissue explants were placed in 4% PFA (Electron Microscopy Sciences, Hatfield, PA, USA) for 24 h before paraffin embedding.
4
Thymic selection of CD8+ T cells
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FTOC was essentially performed as described previously45 (link). Embryonic mice on day 15 of gestation were obtained from Tap1−/−OT-I TCR transgenic mice. Thymus lobes were cultured on Isopore membrane filters (Millipore) placed on Gelfoam sponge (Pfizer) floated in medium. Peptides were added at a concentration of 20 μM. After 3 days in the culture, thymocytes were stained with antibodies specific for CD4 (GK1.5), CD8 (53-6.7) and Vα2 (B20.1) and analysed using flow cytometry; staining antibodies were obtained from Biolegend and were used at 10 μg ml−1.
5
Induced Myc/NICD for Hair Cell Regeneration
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To induce Myc/NICD in vivo, otic bullas were opened to expose the round window niche. For transient Dox exposure, gelfoam sponge (Pfizer) soaked with Dox in DMSO at the concentration of 50 mg/ml was placed outside the round window niches for 4 days. The gelfoam was surgically removed 2 days before ad-Atoh1 infection.
6
Unilateral Decompressive Craniectomy for Severe Traumatic Brain Injury
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In this study, we retrospectively reviewed the medical records on 287 patients with moderate or severe TBI who had Glasgow Coma Scale (GCS) score of less than 13 on admission and underwent DC from 2007 to 2012. The indication for performing DC was that the brain was markedly swollen after evacuation of any type of intradural hemorrhagic lesion or increased intracranial pressure (ICP) refractory to medical treatment in patients with ICP monitoring. The DC procedure entailed removal of a large portion of skull bone. The underlying dura was opened widely for outward expansion of the swollen brain. Augmentation duraplasty with artificial dural substitute was performed in some patients. In other patients, the dura was left open and the exposed brain surface was covered with Gelfoam sponge (Pfizer, New York, NY, USA).
Among them, 38 patients who had incomplete medical records or lacked postoperative computed tomography (CT) scan were excluded from study. To avoid confounding factors, patients who had no obvious midline shift due to diffuse brain swelling or bilateral hematomas, penetrating brain injury or a history of cerebral infarction prior to their injury were excluded. Finally, 173 patients with moderate or severe TBI were enrolled to determine the risk factors for the development of PTCI following unilateral DC.
Among them, 38 patients who had incomplete medical records or lacked postoperative computed tomography (CT) scan were excluded from study. To avoid confounding factors, patients who had no obvious midline shift due to diffuse brain swelling or bilateral hematomas, penetrating brain injury or a history of cerebral infarction prior to their injury were excluded. Finally, 173 patients with moderate or severe TBI were enrolled to determine the risk factors for the development of PTCI following unilateral DC.
7
Cerebellar Aspiration Lesion in Mice
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Anaesthetized (100 mg/kg ketamine and 20 mg/kg xylazine, i.p.) mice were secured in a stereotaxic frame with non-puncturing ear bars. The muscles on the right (or left) side of the cranium were cut to clear the occipital bone of any tissue. A micro Trephine drill (Fine Science Tools, Heidelberg, Germany) was used to create a circular craniotomy of 5 mm in the occipital part of the skull, the dura was punctured, and the right (or left) cerebellar hemisphere and right (or left) hemivermis were ablated by aspiration. Extra caution was taken to avoid lesioning extracerebellar structures. The remaining cavity was filled with sterile GelFoam Sponge (Pfizer, Puurs, Belgium). The skull was closed with a layer of Bone Wax (Fine Science Tools) and carboxylate luting cement (Durelon TM , 3M ESPE AG, Seefeld, Germany). Finally, the skin was sutured and the animal was allowed to recover from anaesthesia and surgery under an infra-red heating lamp. The overall well-being of the mice was monitored daily during the first week post-surgery and if necessary analgesia was administered (i.p. injection of 0.05 mg/kg buprenorphine, Vetergesic, Ecuphar, Oostkamp, Belgium). The MWM acquisition trials started 4 weeks after surgery.
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