Briefly, HEK-293 cells transfected with/without mouse GPRC6A cDNA plasmid were starved by overnight incubation in serum-free DMEM/F12 containing 0.1% bovine serum albumin (BSA) and stimulated with various ligands at different doses. ERK activation were assessed 20 min after treatment by using ERK1/2 (phospho-T203/Y204) ELISA Kit (Invitrogen) corrected for the amount of total ERK using ERK1/2 (Total) ELISA Kit (Invitrogen) to measure ERK levels.
Erk1 2 total elisa kit
Manufactured by Thermo Fisher Scientific
The ERK1/2 (Total) ELISA Kit is a quantitative immunoassay designed to measure the total levels of extracellular signal-regulated kinases 1 and 2 (ERK1/2) in cell and tissue lysates.
Automatically generated - may contain errors
4 protocols using erk1 2 total elisa kit
1
GPRC6A-Mediated ERK Activation in HEK-293 Cells
2
Interrogating GPRC6A signaling in HEK-293 cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Briefly, HEK‐293 cells transfected with wild type mouse GPRC6A (mGPRC6AICL3_KGRKLP) and mGPRC6AICL3_KGKY mutant cDNA plasmids were starved by overnight incubation in serum‐free DMEM/F12 containing 0.1% bovine serum albumin (BSA) and stimulated with various ligands at different doses. ERK activation were assessed the time as indicated after treatment by using ERK1/2 (phospho‐T203/Y204) ELISA Kit (Invitrogen) corrected for the amount of total ERK using ERK1/2 (Total) ELISA Kit (Invitrogen) to measure ERK levels. mTOR activation were assessed by using mTOR ELISA Kit (abcam).
3
Investigating GPRC6A-Mediated ERK Activation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Briefly, HEK‐293 cells transfected with/without mouse GPRC6A cDNA plasmid were starved by overnight incubation in serum‐free DMEM/F12 containing 0.1% bovine serum albumin (BSA) and stimulated with various ligands at different doses. ERK activation were assessed 20 min after treatment by using ERK1/2 (phospho‐T203/Y204) ELISA Kit (Invitrogen) corrected for the amount of total ERK using ERK1/2 (Total) ELISA Kit (Invitrogen) to measure ERK levels.
4
Osteocalcin Activation of GPRC6A
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ocn was purified from bovine tibial bone extracts (41, 42) . Decarboxylated Ocn was produced by treating Ocn in vacuo at 110°C (42) (43) (44) . The purity and decarboxylation state were confirmed by native gel electrophoresis (41), or by blotting followed by reaction with 4-diazobenzene sulfonic acid staining for γ-carboxyglutamic acid (42, 43) . The human Ocn fragments were synthesized by BioMatik USA (Wilmington, Delaware, USA).
All culture reagents were from Invitrogen (Waltham, MA, USA). Human embryonic kidney HEK-293 cells were obtained from American Type Culture Collection and cultured in DMEM medium supplemented with 10% fetal bovine serum and 1% Penicillin/Streptomycin (P/S). Briefly, HEK-293 cells transfected with/without human GPRC6A isoforms cDNA plasmids were starved by overnight incubation in serum-free DMEM/F12 containing 0.1% bovine serum albumin (BSA)
and stimulated with various ligands at different doses. ERK activation was assessed 20 min after treatment by using ERK1/2 (phospho-T203/Y204) ELISA Kit (Invitrogen) corrected for the amount of total ERK using ERK1/2 (Total) ELISA Kit (Invitrogen) to measure ERK levels.
All culture reagents were from Invitrogen (Waltham, MA, USA). Human embryonic kidney HEK-293 cells were obtained from American Type Culture Collection and cultured in DMEM medium supplemented with 10% fetal bovine serum and 1% Penicillin/Streptomycin (P/S). Briefly, HEK-293 cells transfected with/without human GPRC6A isoforms cDNA plasmids were starved by overnight incubation in serum-free DMEM/F12 containing 0.1% bovine serum albumin (BSA)
and stimulated with various ligands at different doses. ERK activation was assessed 20 min after treatment by using ERK1/2 (phospho-T203/Y204) ELISA Kit (Invitrogen) corrected for the amount of total ERK using ERK1/2 (Total) ELISA Kit (Invitrogen) to measure ERK levels.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!