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Anti cd98 pe

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Anti CD98-PE is a fluorescent-labeled antibody that binds to the CD98 cell surface antigen. CD98 is a transmembrane glycoprotein expressed on a variety of cell types, including activated lymphocytes, monocytes, and certain tumor cells. The PE (phycoerythrin) fluorescent label allows for the detection and analysis of CD98-expressing cells using flow cytometry.

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Lab products found in correlation

Lsr 2 flow cytometer, by BD (2 mentions) Celltrace violet, by Thermo Fisher Scientific (2 mentions) Anti cd8 apc efluor780, by Thermo Fisher Scientific (2 mentions) Anti cd62l pe cy7, by Thermo Fisher Scientific (2 mentions) Anti cd44 apc, by Thermo Fisher Scientific (2 mentions) Apc brdu flow kit, by BD (2 mentions) Moflow, by Beckman Coulter (2 mentions) Anti cd69 percpcy5, by Thermo Fisher Scientific (2 mentions) Anti cd4 bd605nc, by BD (2 mentions)

2 protocols using anti cd98 pe

1

Multiparameter Flow Cytometry Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies from eBiosciences included anti-CD8-APC-eFluor780 (47-0081), anti-CD44-APC (17-0441-81), anti-CD69-PERCPcy5.5 (45-0691), anti CD98-PE (12-0981-81; eBiosciences), and anti CD62L-PEcy7 (25-0621). Anti-CD4-BD605NC was acquired from BD Biosciences (San Jose, CA). Cells were stained for 20 minutes at 4°C in PBS, 5% BSA, 0.1% NaN3. Cell proliferation was assessed using Cell Trace Violet (C34557; Life Technologies) according to the manufacturer’s recommendations. Samples were acquired on a BD LSRII flow cytometer and analyzed with Treestar FlowJo software. Cell cycle analysis was performed using BD APC BrdU Flow kit per the manufacturer’s instructions (552598; BD Biosciences). Cells were sorted on a MoFlow (Beckman-Coulter) or Reflection (i-Cyt).
Verbist K.C., Guy C.S., Milasta S., Liedmann S., Kamiński M.M., Wang R, & Green D.R. (2016). Metabolic Maintenance of Cell Asymmetry following Division in Activated T Lymphocytes. Nature, 532(7599), 389-393.
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2

Multiparameter Flow Cytometry Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies from eBiosciences included anti-CD8-APC-eFluor780 (47-0081), anti-CD44-APC (17-0441-81), anti-CD69-PERCPcy5.5 (45-0691), anti CD98-PE (12-0981-81; eBiosciences), and anti CD62L-PEcy7 (25-0621). Anti-CD4-BD605NC was acquired from BD Biosciences (San Jose, CA). Cells were stained for 20 minutes at 4°C in PBS, 5% BSA, 0.1% NaN3. Cell proliferation was assessed using Cell Trace Violet (C34557; Life Technologies) according to the manufacturer’s recommendations. Samples were acquired on a BD LSRII flow cytometer and analyzed with Treestar FlowJo software. Cell cycle analysis was performed using BD APC BrdU Flow kit per the manufacturer’s instructions (552598; BD Biosciences). Cells were sorted on a MoFlow (Beckman-Coulter) or Reflection (i-Cyt).
Verbist K.C., Guy C.S., Milasta S., Liedmann S., Kamiński M.M., Wang R, & Green D.R. (2016). Metabolic Maintenance of Cell Asymmetry following Division in Activated T Lymphocytes. Nature, 532(7599), 389-393.
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