Minoxidil

Manufactured by Tokyo Chemical Industry

Sourced in Japan

Minoxidil is a laboratory instrument designed for the measurement and analysis of chemical compounds. It is used to determine the concentration and purity of various substances. The core function of Minoxidil is to provide accurate and reliable data to support scientific research and product development.

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Lab products found in correlation

Isogen kit, by Nippon Gene (2 mentions) Nanodrop 2000 spectrophotometer, by Thermo Fisher Scientific (2 mentions) Xav939, by Merck Group (1 mentions) Ripa buffer, by Merck Group (1 mentions) 2 d quant kit, by GE Healthcare (1 mentions) D tubocurarine, by Merck Group (1 mentions) Glibenclamide, by Tokyo Chemical Industry (1 mentions)

8 protocols using minoxidil

Propolis Induces Hair Follicle Anagen in Mice

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Using a model of gentle anagen induction [5 (link)], mice were anesthetized at 8 weeks of age (telogen stage) and a 12cm² area (horizontal length,
3 cm; longitudinal length, 4 cm) on the skin aspect of the back was shaved using an electric shaver. Next, 200 µl of respective treatments namely, (i) 50% ethanol (control
group), (ii) 99.5% ethanolic extract of propolis diluted two-fold in distilled water (EEP group) and (iii) 1% minoxidil (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan) dissolved in 50%
ethanol (minoxidil group) were topically administered onto the shaved area for a consecutive period of 28 days starting at day 2 post-induction (pi) up to day 30
pi. The effect of each treatment was observed for an extended period of 22 days (day 52 pi). Mice were sacrificed at two different time points
(n=6/group/time point): day 20 pi and day 52 pi (Fig. 1aFig. 1.

The Philippines Stingless bee propolis induces C57BL/6N female mice skin hair follicles (HFs) into the anagen stage. (A) Experimental time course. (B) Representative images of mice
applied with various treatment at the 0 day, 20th day, and 52nd day respectively. (C) Hair length of the dorsal skin. Values are mean ± SD (*P<0.05 and
**P<0.01).

Tang Y., Wang C., Desamero M.J., Kok M.K., Chambers J.K., Uchida K., Kominami Y., Ushio H., Cervancia C., Estacio M.A., Kyuwa S, & Kakuta S. (2022). The Philippines stingless bee propolis promotes hair growth through activation of Wnt/β-catenin signaling pathway. Experimental Animals, 72(1), 132-139.

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Hair Follicle Dermal Papilla Cells Response to TCQA

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HFDPCs were seeded at a density of 5×10⁵ cells per 100-mm petri dish. The cells were allowed to attach overnight and then the growth medium was replaced with a fresh one containing 0 and 10 µM of TCQA or 0.1 µM minoxidil used as positive control (Tokyo Chemical Industry, Tokyo, Japan). HFDPCs were treated as well with only 10 µM XAV939 (SIGMA, Saint Louis, USA), then with 10 µM XAV939 following by a further incubation with 10 µM TCQA (XAV939/TCQA), and finally co-treated with 10 µM XAV939 and TCQA simultaneously (XAV939+TCQA). After 6 and 12 h treatment, the growth medium was removed, and the cells washed with cold PBS before the total RNA was extracted using ISOGEN kit (Nippon Gene, Tokyo, Japan) following the manufacturer’s instructions. The RNA concentration was assessed using a NanoDrop 2000 spectrophotometer (NanoDrop Technologies, Massachusetts, USA).

Bejaoui M., Villareal M.O, & Isoda H. (2019). β-catenin-mediated hair growth induction effect of 3,4,5-tri-O-caffeoylquinic acid. Aging (Albany NY), 11(12), 4216-4237.

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Synthesis of 5,6-Dichloroindirubin-3'-Methoxime

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KY19382 (5, 6‐dichloroindirubin‐3′‐methoxime) was synthesized in our laboratory (Choi, Kim et al., 2019). Minoxidil was supplied by Tokyo Chemical Industry Co. (Tokyo, Japan) and 2,2,2‐tribromoethanol by Sigma Aldrich (St. Louis, MO, USA).

Ryu Y.C., Lee D., Shim J., Park J., Kim Y., Choi S., Bak S.S., Sung Y.K., Lee S, & Choi K. (2021). KY19382, a novel activator of Wnt/β‐catenin signalling, promotes hair regrowth and hair follicle neogenesis. British Journal of Pharmacology, 178(12), 2533-2546.

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Hair Follicle Dermal Papilla Cells Assay

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HFDPCs were seeded in a collagen-coated 6-well plate with a density of 3 × 10⁴ and left overnight to attach. Cells were treated with 1/2000 dilution of BT-GC and BT-OC, 0.1 μM minoxidil (Tokyo Chemical Industry, Tokyo, Japan) as a positive control, and 0.1 μM, 0.5 μM, 1 μM, 2.5 μM, or 10 μM of me-meijicoccene and C32 botryococcene for 48 h. After 48 h, the total RNA was extracted using ISOGEN kit (Nippon Gene, Tokyo, Japan) according to the manufacturer’s instructions and was quantified using the NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific Inc., Wilmington, DE, USA).

Oliva A.K., Bejaoui M., Hirano A., Arimura T., Linh T.N., Uchiage E., Nukaga S., Tominaga K., Nozaki H, & Isoda H. (2022). Elucidation of the Potential Hair Growth-Promoting Effect of Botryococcus terribilis, Its Novel Compound Methylated-Meijicoccene, and C32 Botryococcene on Cultured Hair Follicle Dermal Papilla Cells Using DNA Microarray Gene Expression Analysis. Biomedicines, 10(5), 1186.

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Intracellular ATP Quantification Assay

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Luminescence luciferase assay kit (Toyo Ink, Tokyo, Japan) was used to measure the ATP content. HFDPCs were seeded in 96-well plate at 3×10⁵ cells/ 100 µl well for 24 h, then the medium was replaced by fresh culture medium containing various concentrations of TCQA or 0.1 µM of the positive control minoxidil (Tokyo Chemical Industry, Tokyo, Japan). After 24 h and 48 h, the plate was incubated for 15 min at RT, then 100 µl of ATP reagent was added, followed by homogenization, and 1 min incubation in the dark. Then, 150 µl of the suspension was transferred to white 96-well plate and incubated for 10 min at RT. Intracellular ATP content was measured by luminescence and calculated as the percentage (%) of TCQA treated-cells relative to the control (untreated cells).

Bejaoui M., Villareal M.O, & Isoda H. (2019). β-catenin-mediated hair growth induction effect of 3,4,5-tri-O-caffeoylquinic acid. Aging (Albany NY), 11(12), 4216-4237.

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Minoxidil and TCQA In Vitro Assay

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Minoxidil was bought from Tokyo Chemical Industry (Tokyo, Japan). TCQA was synthesized with 97% purity. The two samples were prepared in 70% ethanol prior to dilution in papilla growth medium (as described below) for in vitro assay.

Bejaoui M., Oliva A.K., Ke M.S., Ferdousi F, & Isoda H. (2022). 3D Spheroid Human Dermal Papilla Cell as an Effective Model for the Screening of Hair Growth Promoting Compounds: Examples of Minoxidil and 3,4,5-Tri-O-caffeoylquinic acid (TCQA). Cells, 11(13), 2093.

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Protein Extraction Protocol for HFDPC

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HFDPCs were seeded at density of 5×10⁵ cells per 100-mm petri dish. After overnight incubation, the medium was removed and the cells were treated with 10 µM TCQA and 0.1 µM minoxidil (Tokyo Chemical Industry, Tokyo, Japan). After 12 h and 24 h treatment, total protein extraction was achieved using radio-immunoprecipitation assay (RIPA) buffer (SIGMA, Saint Louis, USA) and protease inhibitor following the manufacturer’s instructions.
Proteins from tissues collected from treated area at mice dorsal part were also extracted. The skin sections were crushed using a homogenizer in RIPA buffer (SIGMA, Saint Louis, USA) and protease inhibitor following the manufacturer’s instructions.
Protein samples (15 µg) were quantified using 2-D Quant kit according to manufacturer’s instructions (GE Healthcare, Chicago, USA).

Bejaoui M., Villareal M.O, & Isoda H. (2019). β-catenin-mediated hair growth induction effect of 3,4,5-tri-O-caffeoylquinic acid. Aging (Albany NY), 11(12), 4216-4237.

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Vascular Smooth Muscle Contractility

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During experiments, tissues were maintained in Krebs’ solution consisting of (mM): NaCl 118.4, KCl 4.7, CaCl₂ 2.5, MgSO₄ 1.2, KH₂PO₄ 1.2,
NaHCO₃ 25 and glucose 11.7. D-tubocurarine was obtained from Sigma-Aldrich (St. Louis, MO, U.S.A.). Glibenclamide and minoxidil were obtained from Tokyo Chemical Industry
(Tokyo, Japan). D-tubocurarine was dissolved in distilled water. Glibenclamide and minoxidil were dissolved in DMSO. The highest concentration of vehicles (1%) for the drugs alone had no
effect on the basal tone and contractile responses. The concentrations of drugs given were final concentrations in the bath solution. Glibenclamide and minoxidil were applied
accumulatively.

HORII K., SUZUKI Y., SHIINA T., SAITO S., ONOUCHI S., HORII Y., SHIMAOKA H, & SHIMIZU Y. (2019). ATP-dependent potassium channels contribute to motor regulation of esophageal striated muscle in rats. The Journal of Veterinary Medical Science, 81(9), 1266-1272.

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