AMH was measured in duplicate by Gen II ELISA following the manufacturer’s instructions (Beckman Coulter, Brea, CA, USA). All other hormonal laboratory tests were measured by Esoterix Laboratories (Calabasas Hills, CA, USA): DHEAS by radioimmunoassay after hydrolysis; E2, androstenedione and total T by high-performance liquid chromatography with tandem mass spectrometry; and free T by equilibrium dialysis.
Gen 2 elisa
Manufactured by Beckman Coulter
Sourced in United States
The Gen II ELISA is a laboratory equipment designed for enzyme-linked immunosorbent assay (ELISA) analysis. It functions as a platform for the detection and quantification of various analytes, such as proteins, hormones, and antibodies, through a colorimetric or fluorometric signal generated by the enzymatic reaction.
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Lab products found in correlation
16 protocols using gen 2 elisa
1
Hormonal Assay Protocol for AMH
2
Serum AMH Measurement Protocols
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Serum AMH levels were measured in cases and in the two control cohorts in the course of several years by two types of commercially available immunoenzymatic assays: the GenII ELISA (Beckman Coulter) and the EIA AMH/MHS kit (Immunotech, Beckman Coulter). The limit of detection (LOD) of both assays was 0.14 ng/ml. For participants with measurements below the LOD, values were replaced with LOD/√2 (15 (link)). AMH was detected in 8% of cases and 2.5% of controls. Intra-assay and inter-assay coefficients of variation were 12.3 and 14.2%, respectively.
3
Puberty Biomarkers in Childhood
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Children provided fasting blood samples during the follow-up visit at age 8–14 years. Serum aliquots were separated and frozen at −80 °C, and then sent to the Clinical Ligand Assay Service Satellite (CLASS) Laboratory at the University of Michigan (Ann Arbor, MI) for hormone analysis. We measured estradiol, testosterone, inhibin B, and sex hormone-binding globulin (SHBG) as biomarkers of puberty, and dehydroepiandrosterone sulfate (DHEA-S) as a biomarker of adrenarche. Estradiol, total testosterone, SHBG, and DHEA-S were measured using an automated chemiluminescent immunoassay (Bayer Diagnostics ACS:180). Active inhibin B was assayed using Gen II ELISA (Beckman Coulter, Webster, TX). Values below the limit of detection (LOD) were replaced with the LOD/√2.
4
In-Depth Fertility Assessment Protocol
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The study was initiated following ethical approval, and all eligible patients who provided consent were enrolled. Eligibility for the study subjects and controls was assessed after evaluating the inclusion/exclusion criteria. For the study group, a comprehensive history was obtained, including details of previous IVF cycles, response to stimulation, and outcomes. Clinical examinations were performed, which included measurements of weight, height, and BMI. Pre-treatment baseline hormone assays (day 2/3) were conducted in all patients encompassing serum measurements of FSH, LH, and estradiol. Serum AMH was evaluated in the same sample using Gen II ELISA (Beckman Coulter Diagnostics, USA). Pre-treatment investigations were done in all women including evaluation of serum TSH and serum prolactin. A baseline transvaginal ultrasound (day 2/3) was done to evaluate antral follicle count (AFC) and ovarian volume. Pre-IVF assessment of uterine cavity (3D imaging, hysteroscopy/4D ultrasound, and mock embryo transfer) and fallopian tubes (to rule out hydrosalpinx) was done besides endometrial thickness measurement (days 14–18).
5
Puberty Biomarker Measurement Protocol
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Children provided fasting blood samples during the early-teen visit at age 8–14 years. Serum aliquots were separated and frozen at− 80 °C, and then sent to the Clinical Ligand Assay Service Satellite (CLASS) Laboratory at the University of Michigan (Ann Arbor, MI) for hormone analysis. Estradiol, testosterone, inhibin B, and sex hormone-binding globulin (SHBG) were measured in serum samples as biomarkers of puberty, and dehydroepiandrosterone sulfate (DHEA-S) was measured as a biomarker of secretion of adrenal androgens. Estradiol, total testosterone, SHBG, and DHEA-S were measured using an automated chemiluminescent immunoassay (Bayer Diagnostics ACS:180). Active inhibin B was assayed using Gen II ELISA (Beckman Coulter, Webster, TX). All laboratories that performed hormone analyses employed standard quality control (QC) measures, including the use of blanks and duplicate samples to measure instrument precision and identify potential sources of contamination at different collection and measurement stages. The samples were calibrated with standards to determine the degree of bias and implement actions to prevent calibration drift. The laboratories also followed pre-specified protocols for samples that exceed QC activity control limits. Values below the LOD were replaced with the LOD/√2.
6
Pubertal Biomarker Analysis in Youth
7
Biomarkers of Pubertal Development
8
Puberty and Adrenarche Biomarkers
9
Hormone Assay and Assisted Reproduction
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Gen II ELISA (Beckman Coulter Inc., USA, catalog number A79765/A79766, unmodified version). (Beckman Coulter, USA) kit was used to estimate hormone levels (FSH-Cat. No. 33520 Access hFSH reagent, 100 determinations, 2 × 50 tests); E2 (Cat. No. B84493 Access Sensitive Estradiol Reagent, 100 determinations) and AMH (Cat. No. B13127 Access AMH Reagent, 100 determinations, 2 × 50 tests) from the blood serum according to manufacturer’s instructions. Insemination and intra cytoplasmic sperm injection (ICSI), oocyte retrieval, culture, fertilization, embryo culture, and transfer were carried out as previously described by Gardner et al. (2001) (link).
10
Preoperative Hormone Assessment in Hysterectomy
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A preoperative blood sample was taken during hysterectomy and oophorectomy to assess the serum levels of antim€ ullerian (AMH), follicle-stimulating, and luteinizing hormones; estrogen (estradiol); progesterone; testosterone (total and free fraction); and sex hormone binding globulin. The immunoassay system E170 Modular (Roche Diagnostics, Mannheim, Germany) was used for all hormone assessments except for the assessment of serum AMH levels, where the enzyme-linked immunoassay (Gen II ELISA; Beckman Coulter, Brea, CA) was used. Serum hormone levels are shown in Table 1.
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