Mouse il 10 elisa max standard kit

Manufactured by BioLegend

Sourced in United States

The Mouse IL-10 ELISA MAX Standard Kit is a tool designed to quantify the levels of interleukin-10 (IL-10) in mouse samples using the enzyme-linked immunosorbent assay (ELISA) method. The kit provides the necessary components to perform the assay, including the standard, detection antibody, and substrate solutions.

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Lab products found in correlation

Synergy ht microplate reader, by Agilent Technologies (1 mentions)

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IL-10 (167 citations) ELISA MAX kits (48 citations) Mouse ELISA kits (37 citations) ELISAs (15 citations) Mouse IL-10 (4 citations)

4 protocols using mouse il 10 elisa max standard kit

Quantifying Secreted IL-10 by ELISA

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Secreted IL-10 levels in the cultured supernatant were measured by Mouse IL-10 ELISA MAX Standard Kit (BioLegend, San Diego, CA, USA) following the manufacturer’s manual. All samples were immediately 1:1 diluted prior to measure and measured in duplicate. Absorbance values were read by Synergy HT Microplate Reader (Biotek, Winooski, VT, USA) at 450 nm and IL-10 concentrations were calculated according to standard curve and dilution ratio.

LIU Z., HU Y., YU P., LIN M., HUANG G., KAWAI T., TAUBMAN M., WANG Z, & Xiaozhe H. (2017). Toll-like receptor agonists Porphyromonas gingivalis LPS and CpG differentially regulate IL-10 competency and frequencies of mouse B10 cells. Journal of Applied Oral Science, 25(1), 90-100.

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Quantifying IL-10 in Cell Supernatant

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Cell culture supernatant were collected and IL-10 level in the supernatant was detected using a Mouse IL-10 ELISA MAX Standard kit (Biolegend).

Lin M., Lin J., Wang Y., Bonheur N., Kawai T., Wang Z, & Han X. (2015). Lipopolysaccharide Attenuates CD40 Ligand-Induced Regulatory B10 Cell Expansion and IL-10 Production in Mouse Splenocytes. Open journal of immunology, 5(1), 1-8.

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Measuring IL-10 in Cell Culture

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The secreted IL-10 levels in the cultured supernatant were measured by Mouse IL-10 ELISA MAX Standard Kit (BioLegend) following the manufacturer’s manual and the detection limit is 15.6 pg/ml.

Hu Y., Yu P., Yu X., Hu X., Kawai T, & Han X. (2017). IL-21/anti-Tim1/CD40 ligand promotes B10 activity in vitro and alleviates bone loss in experimental periodontitis in vivo. Biochimica et biophysica acta, 1863(9), 2149-2157.

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Quantifying IL-10 in Cell Supernatant

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Cell culture supernatant were collected and IL-10 level in the supernatant was detected using a Mouse IL-10 ELISA MAX Standard kit (Biolegend).

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