Polyclonal PIPKIγ anti-serum was generated by immunizing rabbits using purified His-tagged mouse PIPKIγ and purified using a human PIPKIγ-conjugated affinity column to generate the anti-pan-PIPKIγ antibody as described 9 (link). To generate the phosphorylated-PIPKIγ antibody, we immunized rabbits with a PIPKIγ phosphopeptide (CDIpYFPTDERSWVYSPLHYSA). The anti-sera were collected, pre-cleaned by a non-phosphopeptide (CDIYFPTDERSWVYSPLHYSA) affinity column, then purified using an affinity column conjugated with the phosphopeptide. Antibodies: anti-HA and MMP9 antibodies (Millipore, Billerica, MA); pERK1/2 and ERK1/2 antibodies (Cell Signaling, Danvers, MA); β-actin antibody and monoclonal anti-α smooth muscle actin antibody (Sigma, St. Louis, MO); Rabbit anti-Cytokeratin 8 antibody, Rat monoclonal anti-CD34 antibody and monoclonal anti-CD68 antibody (Abcam, Cambridge, MA); Alexa Fluor 488 goat anti-mouse antibody, Alexa Fluor 555 goat anti-rabbit antibody and Alexa Fluor 555 goat anti-rat antibody (Molecular probes).
Rat monoclonal anti cd34 antibody and monoclonal anti cd68 antibody
Manufactured by Abcam
Rat monoclonal anti-CD34 antibody and monoclonal anti-CD68 antibody are laboratory reagents used for the detection and analysis of specific cellular markers. The anti-CD34 antibody targets the CD34 protein, a marker expressed on hematopoietic stem and progenitor cells. The anti-CD68 antibody recognizes the CD68 antigen, a glycoprotein found predominantly in monocytes and macrophages. These antibodies are intended for use in various immunological techniques, such as flow cytometry, immunohistochemistry, and western blotting, to facilitate the identification and characterization of cell populations expressing the respective markers.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 goat anti mouse antibody, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 555 goat anti rabbit antibody, by Thermo Fisher Scientific (2 mentions)
Perk1 2 and erk1 2 antibodies, by Cell Signaling Technology (2 mentions)
Anti ha and mmp9 antibodies, by Merck Group (2 mentions)
β actin antibody and monoclonal anti α smooth muscle actin antibody, by Merck Group (2 mentions)
Rabbit anti cytokeratin 8 antibody, by Abcam (2 mentions)
Alexa fluor 555 goat anti rat antibody, by Thermo Fisher Scientific (2 mentions)
2 protocols using rat monoclonal anti cd34 antibody and monoclonal anti cd68 antibody
1
Generation of Anti-PIPKIγ Antibodies
2
Generation of Anti-PIPKIγ Antibodies
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