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12 protocols using tetramethylammonium hydroxide tmah

1

Functionalization of MWCNTs with Diverse Thiol Ligands

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MWCNTs (95%
carbon assay, 6–9 nm × 5 μm (outer diameter ×
length)), ethylenediamine (EDA), N,N′-dicyclohexylcarbodiimid (DCC), copper(I) acetate (Cu(OAc)),
indium(III) acetate (In(OAc)3), zinc acetate (Zn(OAc)2), stearic acid (SA), sulfur (S), 1-dodecanethiol (DDT), 1-octadecene
(ODE), oleylamine (OAm), 8-amino-1-octanethiol-hydrochloride (AOT),
2-(dimethylamino)-ethanethiol hydrochloride (DMA), 2-(diethylamino)-ethanethiol
hydrochloride (DEA), 3-mercaptopropionic acid (MPA), 11-mercaptoundecanoic
acid (MUA), l-glutathione (l-GLU), and tetramethylammonium
hydroxide (TMAH) were all purchased from Sigma Aldrich. Cysteamine
hydrochloride (CYS), conc. nitric acid (HNO3, w = 65%), chloroform (CHCl3), n-hexane,
methanol (MeOH), and toluene were obtained from Carl Roth. All reagents
were used as received without any prior purification step.
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2

Synthesis and Functionalization of Magnetic Nanoparticles

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Iron (III) chloride hexahydrate (97%) (FeCl3·6H2O), tetramethylammonium hydroxide (TMAH) (25%), and (3-aminopropyl) triethoxysilane (APTES) (98%) were purchased from Sigma-Aldrich (Burlington, MA, USA). Iron (II) chloride tetrahydrate (98%) (FeCl2·4H2O), glutaraldehyde (25%) (GLU), and sodium hydroxide (NaOH) (98%) were obtained from PanReac AppliChem (Barcelona, Spain). Commercial silica dioxide nanoparticles (100 nm) were acquired from Guangzhou Hongwu Material Technology Co., Ltd. (Guangzhou, China). The ice nucleation protein was acquired from the Snowmax company. Magnetite nanoparticles were synthetized according to the process explained in the next section.
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3

Synthesis of Metal-Organic Framework Photocatalysts

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2-Aminoterephthalic acid (H2-BDC-NH2) linker, N,N-dimethylformamide (DMF), zirconium chloride (ZrCl4), concentrated HCl, and ethanol were purchased from Sigma–Aldrich. Bismuth nitrate pentahydrate (Bi(NO3)3·5H2O), sodium sulfide (Na2S·9H2O), ethylene glycol (EG), and carbamide were purchased from Sigma–Aldrich. Ammonium molybdate tetrahydrate (NH4)6Mo7O24·4H2O and thioacetamide (C2H5NS, 99%) were purchased from Alfa Aesar. 5,5′-Dithiobis(2-nitrobenzoic acid) (DTNBA, 99%), silver nitrate (AgNO3, 99%), sodium hydroxide (NaOH), sodium borohydride (NaBH4, 99.99% metals basis), and tetramethylammonium hydroxide (TMAH, 23%) were purchased from Sigma Aldrich. All chemicals were used without further purification. Methylene blue dye (MB) was used to determine the photocatalytic activity of the prepared photocatalysts.
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4

Online Derivatization of Shale Oil

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Sodium bituminosulfonate (Ichthyol-Natrium Hell®) as well as the unsulfonated API starting materials “vacuum distillate (shale oil), light” (EC 924–360-1; in the following abbreviated as “distillate”) as well as “vacuum distillate (shale oil), light, base and acid treated” (EC 923–317-4; in the following abbreviated as “refined precursor”) from the same production process were provided by Ichthyol-Gesellschaft Cordes, Hermanni & Co. (GmbH & Co.) KG, Germany.
For the online derivatization, tetramethylammonium hydroxide (TMAH) (25% solution in water, Sigma-Aldrich, USA), methanol (> 99.9%, Roth, Karlsruhe, Germany), and purified water (Milli-Q® water purification system (Merck, Darmstadt, Germany)) were used.
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5

Synthesis of Functionalized Magnetic Nanoparticles

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Iron (II) chloride tetrahydrate (98%) (FeCl2*4H2O), glutaraldehyde (25%), and sodium hydroxide (NaOH) (98%) were obtained from PanReac AppliChem (Spain). Iron (III) chloride hexahydrate (97%) (FeCl3*6H2O), tetramethylammonium hydroxide (TMAH) (25%), (3-aminopropyl) triethoxysilane (APTES) (98%), and L-cysteine (Cys) (97%) were purchased from Sigma-Aldrich (USA). In addition, 3-(triethoxysilyl) propylsuccinic anhydride (CAS) (95%) was purchased from Shanghai Kayi Chemical Co. (Shanghai, China).
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6

Pyrolysis-TMAH-GC-MS Analysis of Sorghum Biomass

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Stover samples (∼0.2 mg) from WT and bmr lines were pyrolyzed in a Varian 1079 programmable temperature vaporization (PTV) injector mounted on a Varian 3800 gas chromatograph connected to a Varian 1200 mass spectrometer. The PTV injector was operated at a split ratio of 1:100 at 450°. The pyrolysate was led on a capillary column (25 m, 0.32 mm i.d., fused silica coated with SGE BPX5). Helium (1.2 ml/min) was used as the carrier gas. The GC program started with a hold at 70° for 4 min, followed by a temperature increase to 150° at a rate of 2°/min. The temperature was subsequently increased to 250° at a rate of 4°/min, then to 320° at a rate of 30°/min. The mass spectrometer was operated at 1.0 kV. The mass range included m/z 45 to 350 and was scanned every 0.2 sec. The identification of compounds was based on Ralph and Hatfield (1991) . To compare the proportion of the hydroxycinnamic acids p-coumaric acid and ferulic acid in the biomass, stover was subjected to pyrolysis-TMAH-GC-MS as described by Saballos et al. (2012) (link). Samples of approximately 0.2 mg stover from BTx623 and the novel bmr mutants were trans-methylated with 2.5% (v/v) tetramethyl ammonium hydroxide (TMAH; Sigma-Aldrich, St. Louis, MO) in methanol (Mulder et al. 1992) and pyrolyzed in a Varian 1079 PTV injector mounted on a Varian 3800/1200 GC-MS as described above.
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7

Synthesis and Characterization of Nanoparticles

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Iron acetylacetonate, manganese acetylacetonate, oleic acid, oleylamine, benzyl ether, hexane, ε-caprolactone, tetrahydrofuran (THF), N-(3-dimethylaminopropyl)-Nese acetylacetonate, N-hydroxysuccinimide, stannous octoate, tetramethylammonium hydroxide (TMAH), heparin, L-glutamine, reactive oxygen species (ROS) assay kit, and sodium pyruvate were all purchased from Sigma-Aldrich Co. (St Louis, MO, USA). PEG with a terminal hydroxyl and carboxylic acid functional groups (COOH-PEG-OH) was purchased from JenKem Technology (Beijing, People’s Republic of China). 1,2-Hexadecanediol was obtained from TCI (Shanghai) Development Co., Ltd (Shanghai, People’s Republic of China). Medium 199, Roswell Park Memorial Institute 1640 medium, fetal bovine serum, penicillin/streptomycin, and nonessential amino acid were purchased from Hyclone, Logan, UT, USA. Count Kit-8 (CCK-8) was obtained from Beyotime Biotechnology Company, Beijing, People’s Republic of China. Endothelial cell growth supplement was purchased from Sciencell, CA, Carlsbad, USA. Six-well plates were obtained from Corning Incorporated, Corning, NY, USA.
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8

Functionalized Alumina Beads for Cd(II) Removal

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Iron (III) chloride hexahydrate (97%) (FeCl3∙6H2O), tetramethylammonium hydroxide (TMAH) (25%), (3-Aminopropyl) triethoxysilane (APTES) (98%) and L-Cysteine (Cys) (97%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Iron (II) chloride tetrahydrate (98%) (FeCl2∙4H2O), glutaraldehyde (25%) (GLU), Cd standard solution 1000 mg/L and sodium hydroxide (NaOH) (98%) were obtained from PanReac AppliChem (Barcelona, Spain). Additionally, 3-(triethoxysilyl) propylsuccinic anhydride (CAS) (95%) was purchased from Shanghai Kayi Chemical Co. (Shanghai, China). Commercial aluminum oxide spheres (Alumina) between 600–800 µm of diameter were acquired from Torrecid (L’Alcora, Castellón, Spain).
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9

Peptide-Mediated Biomaterials for Cell Delivery

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Iron (II) chloride tetrahydrate (98%) and sodium hydroxide (NaOH) (98%) were obtained from PanReac AppliChem. Iron (III) chloride hexahydrate (97%), dimethyl sulfoxide (99.5%), tetramethylammonium hydroxide (TMAH) (25%), (3-aminopropyl) triethoxysilane (APTES) (98%), carboxymethyl cellulose (CMC) (98%), N-[3-(dimethylamino)-propyl]-N′-ethylcarbodiimide hydrochloride (EDC) (98%), and glutaraldehyde (25%) were purchased from Sigma-Aldrich Co. DMEM, RPMI 1640 medium, FBS, and trypsin EDTA were obtained from Biowest. Penicillin/streptomycin (P/S) was purchased from Lonza. BUF2 (BUF2-TRSSRAGLQFPVGRVHRLLRK) and fluorescein isothiocyanate (FITC)-BUF2 were synthetized by the Peptide Synthesis Facility at Pompeu Fabra University and GL Biochem (Shanghai, China). Purification was performed by high-pressure liquid chromatography (>95%), and masses were confirmed via mass spectrometry as we did in a previous work.4 Vero Cells (ATCC® CCL-81), THP-1 Cells (ATCC® TIB-202) were used for citotoxicity and delivery assays. Bacterial strain used was Escherichia coli (ATCC 25922).
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10

Magnetic Polymer Composites Synthesis

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Polymethyl methacrylate (PMMA) sheets, also known as acrylic sheets, were purchased from Surtiacrylicos (Bogota, Colombia), Methyl methacrylate based-glue Veracril ® was obtained from New Stetic (Guarne, Colombia), Ethanol (96%) was acquired from Expert Clean Colombia (Bogota, Colombia), and 345 mT Neodymium cylindrical magnets (ϕ: 6 mm × h 7 mm) were purchased from Constructor Mil Imanes (Bogota, Colombia). 2,2-azino-bis(3-ethylbenzothiazoline-6) sulphonic acid (ABTS), glutaraldehyde (25%), sodium hydroxide (NaOH) (98%), tetramethylammonium hydroxide (TMAH) (25%), and 3-aminopropyl-triethoxysilane (APTES) (98%) were purchased from Sigma-Aldrich (Burlington, MA, USA). Iron (II) chloride tetrahydrate (98%) (FeCl2*4H2O), iron (III) chloride hexahydrate (97%) (FeCl3*6H2O), phenol crystallized (99,5%) (C6H6O) (phenol) and dye Eriochrome Black T (EBt) (C.I. 14645) were obtained from PanReac AppliChem (Castellar del Valles, Spain).
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