Samples were screened by immunohistochemistry performed on non-perfused rat or mouse brain, fixed by immersion with 4 % paraformaldehyde for 1 h and processed as reported [11 (link), 14 (link)]. Immunohistochemistry using a standard avidin–biotin peroxidase method was applied using patients' serum (diluted 1:200) or a commercial rabbit polyclonal anti-MOG antibody (Abcam; ab32760; diluted 1:2000) followed by biotinylated secondary antibodies, goat anti-human IgG (H + L) (Vector Laboratories, Burlingame, CA, USA) and goat anti-rabbit IgG (H + L) (Vector Laboratories, Burlingame, CA, USA) (diluted 1:1000), respectively. To show if hMOG-IgG of different patients with a common myelin staining pattern recognized similar epitopes, rat brain sections were pre-incubated with undiluted hMOG-IgG-positive serum for 3 h followed by biotinylated IgG obtained from the two patients described above and processed as reported [15 (link)].
Ab32760
Manufactured by Abcam
Sourced in United States
Ab32760 is a laboratory equipment product. It is a device designed for use in scientific research and analysis. The core function of this product is to perform a specific task or provide a specific capability within a laboratory setting. No further details about the intended use or features of this product can be provided in an unbiased and factual manner.
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Lab products found in correlation
Goat anti rabbit igg h l, by Vector Laboratories (1 mentions)
Ab28486, by Abcam (1 mentions)
Ab31940, by Abcam (1 mentions)
Ab7794, by Abcam (1 mentions)
Ab78078, by Abcam (1 mentions)
Prolong diamond antifade mountant with 4 6 diamidino 2 phenylindole dapi, by Thermo Fisher Scientific (1 mentions)
Bz x710, by Keyence (1 mentions)
2 protocols using ab32760
1
Immunohistochemical Profiling of Anti-MOG Antibodies
2
Immunohistochemical Analysis of Neural Cell Types
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Immunohistochemistry was carried out to investigate the morphological features of neural cells, the numbers of each cell type, and their glycosylation status. The primary antibodies used were as follows: anti-beta III tubulin (ab78078; Abcam, Cambridge, USA), anti-160 kDa NF medium (ab7794; Abcam), anti-MBP (ab626931; Abcam), anti-MAG (9043S CST, Danvers, USA), anti-MOG (ab32760; Abcam), anti-PLP (ab28486; Abcam), anti-APC (OP80; Merck), anti-Tbr1 (ab31940; Abcam), and fluorescein isothiocyanate (FITC)-conjugated WFA (Vector Laboratories, Burlingame, USA). The sections were incubated with primary antibodies (1/200 dilution) overnight at 4°C, rinsed three times in PBS, and incubated with Alexa Fluor-488 or Alexa Fluor-546 secondary antibodies (1/200 dilution) for 2 h at room temperature. The stained sections were sealed using cover slips and ProLong Diamond antifade mountant with 4',6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific, Waltham, USA). Immunofluorescence signals were observed and evaluated under a fluorescence microscope (BZ-X710, Keyence, Osaka, Japan). Representative immunohistochemistry images from 2–3 different mice per genotype are shown in Figs 4 , 5 , 6 , 7 and 8 .
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