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Anti cpt1b

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Anti-CPT1B is a primary antibody that recognizes the carnitine palmitoyltransferase 1B (CPT1B) protein. CPT1B is an enzyme involved in the transport of long-chain fatty acids into the mitochondria for beta-oxidation. This antibody can be used in various research applications to detect and study the expression of CPT1B.

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Lab products found in correlation

Bca protein assay, by Thermo Fisher Scientific (4 mentions) Anti rabbit, by GE Healthcare (2 mentions) Novex tris glycine sds page, by Thermo Fisher Scientific (2 mentions) Anti fabp3, by LifeSpan BioSciences (2 mentions) Lysis buffer cell extraction buffer, by Thermo Fisher Scientific (2 mentions) Anti goat, by Santa Cruz Biotechnology (2 mentions) Imagequant tl, by GE Healthcare (2 mentions) Ecl prime, by GE Healthcare (2 mentions) Anti actin, by Santa Cruz Biotechnology (2 mentions) Pvdf membrane, by Bio-Rad (2 mentions) Rapamycin, by Merck Group (1 mentions) Rabbit anti dsg2, by Abcam (1 mentions) Anti pgc 1α antibody, by Cell Signaling Technology (1 mentions) Anti pparα, by Abcam (1 mentions) Horseradish peroxidase conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions) Immobilon western chemiluminescent hrp substrate, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Fenofibrate, by Merck Group (1 mentions) Anti gapdh, by Abcam (1 mentions)

4 protocols using anti cpt1b

1

Protein Expression Analysis in Muscle Tissue

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Muscle homogenates were prepared by grinding tissue with ice-cold lysis buffer cell extraction buffer (Invitrogen) as previously described (13 (link)). Protein concentrations were measured using a BCA protein assay (Thermo Scientific). Proteins were separated by 4–20% Novex Tris-Glycine SDS-PAGE (Invitrogen) and transferred to a PVDF membrane (Biorad). Membranes were incubated overnight with anti-FABP3 (Lifespan Biosciences, Seattle, WA; LS-C138955), anti-CPT1B (Abcam, Cambridge, MA; ab15703) or anti-actin (Santa Cruz, Dallas, TX; sc-1616), followed by incubation with appropriate secondary horseradish peroxidase-conjugated antibodies, anti-rabbit (GE Healthcare, Piscataway, NJ; NA931) and anti-goat (Santa Cruz, sc-2020). Immunoreactive proteins were visualized by chemiluminescence reagent (ECL Prime; GE Healthcare) and quantified by densitometric analysis using ImageQuantTL (GE Healthcare).
Mulya A., Haus J.M., Solomon T.P., Kelly K.R., Malin S.K., Rocco M., Barkoukis H, & Kirwan J.P. (2017). Exercise Training Induced Improvement in Skeletal Muscle PGC-1α Mediated Fat Metabolism is Independent of Dietary Glycemic Index. Obesity (Silver Spring, Md.), 25(4), 721-729.
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2

Protein Expression Analysis in Muscle Tissue

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Muscle homogenates were prepared by grinding tissue with ice-cold lysis buffer cell extraction buffer (Invitrogen) as previously described (13 (link)). Protein concentrations were measured using a BCA protein assay (Thermo Scientific). Proteins were separated by 4–20% Novex Tris-Glycine SDS-PAGE (Invitrogen) and transferred to a PVDF membrane (Biorad). Membranes were incubated overnight with anti-FABP3 (Lifespan Biosciences, Seattle, WA; LS-C138955), anti-CPT1B (Abcam, Cambridge, MA; ab15703) or anti-actin (Santa Cruz, Dallas, TX; sc-1616), followed by incubation with appropriate secondary horseradish peroxidase-conjugated antibodies, anti-rabbit (GE Healthcare, Piscataway, NJ; NA931) and anti-goat (Santa Cruz, sc-2020). Immunoreactive proteins were visualized by chemiluminescence reagent (ECL Prime; GE Healthcare) and quantified by densitometric analysis using ImageQuantTL (GE Healthcare).
Mulya A., Haus J.M., Solomon T.P., Kelly K.R., Malin S.K., Rocco M., Barkoukis H, & Kirwan J.P. (2017). Exercise Training Induced Improvement in Skeletal Muscle PGC-1α Mediated Fat Metabolism is Independent of Dietary Glycemic Index. Obesity (Silver Spring, Md.), 25(4), 721-729.
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3

Evaluating Metabolic Regulators in Cell Signaling

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Rapamycin, DMSO and fenofibrate were purchased from Sigma–Aldrich (St. Louis, MO, USA). Rabbit anti-phospho-mTOR (Ser2448), anti-mTOR, anti-phospho-4EBP1 (Thr37/46), anti-4EBP1, anti-PGC1α antibodies and mouse monoclonal anti-β-actin were from Cell Signaling Technology (Beverly, MA, USA). Rabbit anti-DSG2, anti-PPARα, anti-CPT1b, anti-ACADL, anti-GAPDH antibodies were from Abcam Inc. (Cambridge, MA, USA). Horseradish peroxidase-conjugated, donkey anti-rabbit IgG and donkey anti-mouse IgG were from Jackson ImmunoResearch (West Grove, PA, USA). Immobilon western chemiluminescent HRP substrate was purchased from Millipore (Temecula, CA, USA). Trizol reagent and the reverse transcription (RT) system were purchased from Promega Inc. (Madison, WI, USA).
Lin Y., Liu R., Huang Y., Yang Z., Xian J., Huang J., Qiu Z., Lin X., Zhang M., Chen H., Wang H., Huang J, & Xu G. (2022). Reactivation of PPARα alleviates myocardial lipid accumulation and cardiac dysfunction by improving fatty acid β-oxidation in Dsg2-deficient arrhythmogenic cardiomyopathy. Acta Pharmaceutica Sinica. B, 13(1), 192-203.
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4

Biomolecular Extraction and Analysis

Cited 1 time
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Cells and tissues were collected with RIPA lysis buffer and detected as described above [35] (link). The antibodies were as follows: anti-NPRA (Santa Cruz sc-137041), anti-PPARα (Santa Cruz sc-398934), anti-CPT1B (Abcam ab134988), anti-CD44 (Abcam A19020), and anti-CD133 (Abcam A0818).
Cao T., Wang S., Qian L., Wu C., Huang T., Wang Y., Li Q., Wang J., Xia Y., Xu L., Wang L, & Huang X. (2023). NPRA promotes fatty acid metabolism and proliferation of gastric cancer cells by binding to PPARα. Translational Oncology, 35, 101734.
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