Poly (methyl methacrylate-co-methacrylic acid) (PMMA-MA, 1.3% methacrylic acid, Mn ∼15000, Mw ∼34000), acetonitrile (anhydrous, 99.8%), rhodamine B octadecyl ester perchlorate (>98.0%), lithium tetrakis(pentafluorophenyl)borate ethyl etherate were purchased from Sigma-Aldrich. DiD oil (1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate) (Cy5) and quantum dots QD655 were purchased from Life-Technologies. Sodium phosphate monobasic (>99.0%, Sigma-Aldrich) and sodium phosphate dibasic dihydrate (>99.0%, Sigma-Aldrich) were used to prepare 20 mM phosphate buffers at pH 5.8-9.0. Milli-Q water (Millipore) was used in all experiments.
Rhodamine b octadecyl ester perchlorate
Manufactured by Merck Group
Rhodamine B octadecyl ester perchlorate is a fluorescent dye compound used in various laboratory applications. It is a red-colored, lipophilic dye with excitation and emission wavelengths suitable for fluorescence microscopy and other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Milli q water, by Merck Group (3 mentions)
Acetonitrile, by Merck Group (3 mentions)
Pmma ma, by Merck Group (2 mentions)
Lithium tetrakis pentafluorophenyl borate ethyl etherate, by Merck Group (2 mentions)
Did oil 1 1 dioctadecyl 3 3 3 3 tetramethylindodicarbocyanine perchlorate cy5, by Thermo Fisher Scientific (2 mentions)
Quantum dots qd655, by Thermo Fisher Scientific (2 mentions)
Sodium phosphate dibasic dihydrate, by Merck Group (2 mentions)
Sodium phosphate monobasic, by Merck Group (2 mentions)
Bovine liver catalase, by Merck Group (1 mentions)
Sepharose 4b, by Merck Group (1 mentions)
Glucose oxidase, by Merck Group (1 mentions)
Isopropanol, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Cetyltrimethylammonium bromide ctab, by Merck Group (1 mentions)
Hoechst stain solution, by Merck Group (1 mentions)
5 protocols using rhodamine b octadecyl ester perchlorate
1
Polymeric Nanoparticle Synthesis and Characterization
2
Polymeric Nanoparticle Synthesis and Characterization
3
Polymersomes Synthesis and Characterization
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Chemicals were used as received unless otherwise indicated. 2-(Methacryloyloxy)ethyl phosphorylcholine (MPC > 99%) was donated by Biocompatibles. 2-(Diisopropylamino)ethyl methacrylate (DPA) was purchased from Scientific Polymer Products. Copper(I) bromide (CuBr; 99.999%), 2,2′-bipyridine (bpy), methanol (anhydrous, 99.8%), and isopropanol were purchased from Sigma-Aldrich. The silica used for the removal of the atom transfer radical polymerization (ATRP) copper catalyst was column chromatography grade silica gel 60 (0.063 to 0.200 mm) purchased from E. Merck. 2-(N-morpholino)ethyl 2-bromo-2-methylpropanoate (ME-Br) initiator was synthesized according to a previously reported procedure (50 ). Poly(ethylene glycol) methyl ether methacrylate [P(OEG10MA)] was purchased from Sigma-Aldrich. PEO-PBO copolymer was purchased from Advanced Polymer Materials Inc. The polymersomes were labeled using rhodamine B octadecyl ester perchlorate purchased from Sigma-Aldrich. PBS was made from Oxoid tablets (one tablet per 100 ml of water). Bovine liver catalase, glucose oxidase, and glucose have been purchased from Sigma-Aldrich. The gel filtration column for the purification of the polymersomes was made with Sepharose 4B purchased from Sigma-Aldrich.
4
Synthesis and Characterization of PMMA-SO3H Polymers
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Poly(methyl methacrylate-co-3-sulfopropyl methacrylate) polymers (PMMA-SO3H 1 or 2 %) were synthetized through free radical polymerization as described previously. [11, 12, 43] R18/F5-TPB was synthesized from rhodamine B octadecyl ester perchlorate (Sigma-Aldrich, >98.0%) and lithium tetrakis(pentafluorophenyl)borate ethyl etherate (Alfa Aesar, 97%) through ion exchange followed by purification through column chromatography according to the method previously described. [38, 45] Milli-Q water (Millipore), NaCl (≥98%, Sigma-Aldrich), acetonitrile (≥99.9%, Sigma-Aldrich) and Tween 80 (T80, Polysorbate 80, Sigma-Aldrich) were used for preparation of NPs.
5
Lipid-Based Nanoparticle Preparation
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Labrafac PG, Gelucire 50/13 and Compritol 888 atomized, were provided by Gattefossé. Miglyol® 812 N and Imwitor® 948 were a kind gift from IOI Oleo GmbH, Germany. Cetyltrimethylammonium Bromide (CTAB), Hoechst stain solution and Rhodamine B octadecyl ester perchlorate were procured through Sigma-Aldrich. While other reagents were of the analytical grade.
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