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V5 and pan 14 3 3 antibody

Manufactured by Abcam

The V5 and pan 14-3-3 antibody is a laboratory reagent used to detect and analyze proteins in biological samples. The V5 antibody specifically recognizes the V5 epitope tag, while the pan 14-3-3 antibody binds to a variety of 14-3-3 protein isoforms. These antibodies can be used in various applications, such as Western blotting, immunoprecipitation, and immunocytochemistry, to identify and quantify target proteins.

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2 protocols using v5 and pan 14 3 3 antibody

1

Immunoblotting Antibodies for Cell Signaling

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The following antibodies were used in this study: anti-human LNK antibody [AF5888, R&D system Inc, a sheep IgG against E.coli derived recombinant human LNK protein (amino acids 427–575)]; murine anti-β-actin monoclonal antibody (Sigma); V5 and pan 14-3-3 antibody (Abcam); antibodies against p-JAK2 (Tyr1007/1008), JAK2, p-p38 (Thr180/Tyr182), p-ERK1/2 (Thr202/Tyr204), p-JNK1/2 (Tyr183), p-PDK1 (Ser243), p-P70S6 (Thr421/Ser424), p-GSK3beta (Ser9), p-AKT (Ser473) and AKT, p-PI3K p110δ (Tyr485) (from either Cell Signaling Technology or Santa Cruz) and p-FAK Tyr861 (Epitomics).
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2

Immunoblotting Antibodies for Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used in this study: anti-human LNK antibody [AF5888, R&D system Inc, a sheep IgG against E.coli derived recombinant human LNK protein (amino acids 427–575)]; murine anti-β-actin monoclonal antibody (Sigma); V5 and pan 14-3-3 antibody (Abcam); antibodies against p-JAK2 (Tyr1007/1008), JAK2, p-p38 (Thr180/Tyr182), p-ERK1/2 (Thr202/Tyr204), p-JNK1/2 (Tyr183), p-PDK1 (Ser243), p-P70S6 (Thr421/Ser424), p-GSK3beta (Ser9), p-AKT (Ser473) and AKT, p-PI3K p110δ (Tyr485) (from either Cell Signaling Technology or Santa Cruz) and p-FAK Tyr861 (Epitomics).
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