Chromatographic separation was carried out using a FOCUS™ GC instrument, equipped with an AI 3000 autosampler (both Thermo Fisher Scientific, Dreieich, Germany) and a TRACE™ TR-5 capillary column (5% phenyl methyl polysiloxane, 15 m length, 0.25 mm i.d. and 0.25 µm film thickness). The system was operated at a constant carrier gas (helium) flow rate of 1.5 ml·min−1. Focusing of the sample was obtained at 120°C for 1 min, followed by a shallow gradient of 1.5°C·min−1 from 120 to 195°C and a steep gradient of 20°C·min−1from 195 to 300°C, with a final hold of 2 min. Injection of 1 µl sample was performed in split less mode and an inlet temperature of 250°C. The MS transfer line temperature was set to 200°C.
Ai 3000 autosampler
Manufactured by Thermo Fisher Scientific
Sourced in Germany, United States
The AI 3000 autosampler is a laboratory equipment designed for the automated handling and introduction of samples into analytical instruments. It is a core component used to improve efficiency and consistency in sample processing workflows.
Automatically generated - may contain errors
Lab products found in correlation
Focus gc instrument, by Thermo Fisher Scientific (2 mentions)
Dsqii quadrupole mass spectrometer, by Thermo Fisher Scientific (1 mentions)
Trace 1300 gas chromatograph, by Thermo Fisher Scientific (1 mentions)
Cary 60 uv vis spectrophotometer, by Agilent Technologies (1 mentions)
Pb 21 ph meter, by Sartorius (1 mentions)
Lc netii afc interface, by Jasco (1 mentions)
S 4100, by Hitachi (1 mentions)
Asap 2010 automated sorption analyzer, by Micromeritics (1 mentions)
5 protocols using ai 3000 autosampler
1
GC-MS Analysis of Organic Compounds
2
GC-MS Analysis of Metabolites
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Gas chromatography‐mass spectrometry was carried out according to Althammer et al. (2020 (link)) on a Focus™ GC instrument equipped with an AI3000 autosampler and a DSQ™ II quadrupole mass spectrometer (all from Thermo Fisher Scientific, Dreieich, Germany). The separation was performed employing a TRACE™ TR‐5 column (5%‐phenyl‐methylpolysiloxane, 15 m × 0.25 mm i.d., 0.25 µm film thickness) using a constant carrier gas flow rate of 1.5 ml min−1 helium. A 1‐µl sample was injected in splitless injection mode at an inlet temperature of 250°C. First, the analytes were focused at the head of the column with an isothermal phase at 120°C for 1 min, followed by a gradient of 1.5°C min−1 from 120°C to 195°C, a second gradient of 20°C min−1 from 195°C to 300°C, and a final isothermal phase at 300°C for 2 min. The MS transfer line was set to 200°C, ionization was carried out in positive mode applying electron ionization at 70 eV at a temperature of 200°C. The data were acquired in selected ion monitoring mode for the m/z values 204.02, 205.99, 257.00, 298.93, 314.97, 387.04 and 428.95 with an isolation width of m/z ± 0.5 and a dwell time of 10 msec.
3
Quantification of Phenolic Acids in Biological Samples
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Phenolic acids were measured in urine and fermentation fluid. Extraction and derivatisation were carried out as previously described [43 (link)]. Derivatized phenolic acids were analysed on a Trace GC interfaced to a DSQ mass spectrometer equipped with a split/splitless injector and an AI3000 autosampler (Thermo Fisher, Hemel Hempstead, UK) as previously described [42 ]. Identification of phenolic acids was based on retention time (tR) and target ions [49 (link)]. Quantification was based on 2.5 to 15 µg calibration curves of derivatised and analysed phenolic acid standards. The area ratio of each standard was averaged and the coefficient of variance calculated (R2 > 0.98).
4
Rumen Fluid Analysis: pH, NH3-N, and VFAs
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The pH of rumen fluid samples was measured using a digital PB-21 pH meter (Sartorius, Germany). NH3-N concentration was determined according to a colorimetric method using visible-light spectrophotometry (Agilent Cary 60 UV-Vis Spectrophotometer, USA). Volatile fatty acids were separated and quantified by gas chromatography (Focus GC AI 3000 Thermo Finnigan analyzer). One-microliter preprocessed sample was injected using a split (20:1) and using a Thermo Scientific AI 3000 autosampler (USA) on a Thermo Scientific TRACE 1300 Gas Chromatograph (USA). Injector and detector temperatures were both set at 200°C. Initial column temperature was 45°C, and the rate of temperature rise was set at 20°C/min. At 150°C, column temperature was held for 8 min, followed by further heating at 60°C rise/min until 185°C, where it was held for 1 min. The carrier gas used was nitrogen (purity: 99.99%; flow rate: 40.0 ml N2/min).
5
Chromatographic Analysis of Materials
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Chromatographic analysis was carried out in an HPLC equipment from Jasco Analytica (Madrid, Spain), composed of a PU-2089 quaternary gradient pump, an AS-2055 autosampler with a 100 µL injection loop and MD-2018 photodiode array detector. The system was controlled using the LC-NETII/AFC interface also supplied by Jasco. Acquisition and data treatment was performed using the ChromNAV software (version 1.17.01). SEM photographs of PTFE surfaces and monolithic materials were performed with a scanning electron microscope (S-4100, Hitachi, Ibaraki, Japan) provided by a field emission gun, an EMIP 3.0 image data acquisition system, and a microanalysis system (Rontec, Normanton, UK). FT-IR spectra of PTFE surfaces were obtained with a Nicolet Magna FT-IR 750 spectrometer (Madison, WI, USA) fitted with a single reflection attenuated total reflectance (ATR) accessory. Spectra were recorded at room temperature between 4000 and 550 cm -1 with 8 cm -1 nominal resolution at 50 scans per spectrum. Nitrogen adsorption surface area analysis of monolithic materials was performed on a Micromeritics ASAP2010 automated sorption analyzer (Rutherford, Germany). Gas chromatography-mass spectrometry (GC-MS) analysis was performed on a Focus DSQ II gas chromatograph provided with an AI 3000 autosampler and single quadrupole MS detector from Thermo Fisher Scientific (Austin, TX, USA).
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