For immunohistochemistry and fluorescent in situ hybridization rat brains were extracted, washed with PBS and fixed in 4% PFA for 3 hours. After fixation brains were embedded in FSC22 compound (Leica), frozen in liquid nitrogen and stored at −70 °C. Brains were sectioned to 20 µm thick slices on a freezing microtome CM1850UV (Leica). For PSIA–LC–MALDI analysis brains after extraction were immediately frozen in liquid nitrogen and homogenized using a cryogenic laboratory mill Freezer/Mill 6870 (SPEX SamplePrep).
Fsc22 compound
Manufactured by Leica
Sourced in Germany
The FSC22 compound is a general-purpose microscope slide preparation instrument. It is designed to facilitate the creation of high-quality microscope slides for various applications. The FSC22 provides consistent, repeatable slide preparation, enabling standardized sample handling and analysis.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using fsc22 compound
1
Rat Brain Extraction and Preservation
2
Paraformaldehyde Brain Fixation and Sectioning
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Perfusion fixation was carried out prior to dissection, and the brain was fixed in 4% paraformaldehyde (PFA). Brains were embedded in FSC22 compound (Leica). Brain sections (10 μm) were prepared by using a microtome (Leica).
3
Histological Analysis of Mouse Liver Tissue
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For histologic analysis, mouse liver tissues were sliced, embedded onto a specimen disc in frozen section media (FSC 22 compound, Leica, Jena, Germany), and frozen at -20°C. Liver tissue section slides were prepared at a thickness of 8 μm using CM1860 cryostat microtome system (Leica Biosystems, Nussloch, Germany). Sliced liver sections were placed on slide glasses, air-dried for 1 day, and stained with hematoxylin (Mayer's hematoxylin) and eosin (H&E). In addition, sections were separately stained with Oil Red O and hematoxylin to assess lipid deposition.
4
Immunohistochemistry and Protein Fractionation of Animal Brains
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For immunohistochemistry, brains of selected animals were dissected, washed with PBS, and fixed in 4% PFA for 3 h. After rinsing with PBS, brain samples were embedded in FSC22 compound (Leica, Wetzlar, Germany), frozen in liquid nitrogen, and stored at −70 °C. Cryosections of 20 µm thick were made using cryostat CM1850UV (Leica, Wetzlar, Germany). The prepared sections were mounted on glass slides, air-dried, and stored at −20 °C.
For protein fractionation and immunoprecipitation, the brains of the studied animal species were frozen in liquid nitrogen immediately after dissection. The obtained samples were homogenized using a cryogenic laboratory mill Freezer/Mill 6870 (SPEX SamplePrep, St. Metuchen, NJ, USA).
For protein fractionation and immunoprecipitation, the brains of the studied animal species were frozen in liquid nitrogen immediately after dissection. The obtained samples were homogenized using a cryogenic laboratory mill Freezer/Mill 6870 (SPEX SamplePrep, St. Metuchen, NJ, USA).
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