Resipore columns

Manufactured by Agilent Technologies

ResiPore columns are size-exclusion chromatography columns designed for the separation and purification of biomolecules. They are used for the analysis and fractionation of proteins, nucleic acids, and other macromolecules based on their size and molecular weight.

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Lab products found in correlation

Prominence lc 20 ab hplc system, by Waters Corporation (3 mentions) 2410 refractive index detector, by Waters Corporation (2 mentions) Labsolutions software, by Shimadzu (1 mentions) 6130 quadrupole mass spectrometer, by Agilent Technologies (1 mentions) 400 mhz spectrometer, by Agilent Technologies (1 mentions) Agilent 1260 lc system, by Agilent Technologies (1 mentions) Optilab t rex, by Wyatt Technology (1 mentions) Agilent 1260 infinity lc system, by Agilent Technologies (1 mentions)

4 protocols using resipore columns

Molecular Weight Analysis of Polymers

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After drying, retrieved samples were dissolved in tetrahydrofuran (10 mg/ml), filtered (0.45μm filter) and analyzed for the weight average molecular weight using two ResiPore columns (300 X 7.5 mm, 3 μm particle size; Agilent Technologies) in series in a Shimadzu Prominence LC-20 AB HPLC system with Waters 2410 refractive index detector. Polystyrene (Mw: 162 Da -364 kDa, Agilent, Santa Clara, CA) was used as standard and samples were injected at flow rate of 1mL/min at 40 °C [14 (link)].

Venkatesan N., Liyanage A.D., Castro-Núñez J., Asafo-Adjei T., Cunningham L.L., Dziubla T.D, & Puleo D.A. (2019). Biodegradable Polymerized Simvastatin Stimulates Bone Formation. Acta biomaterialia, 93, 192-199.

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Measuring Polymer Molecular Weight

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Molecular weight was measured using a Shimadzu Prominence LC-20 AB HPLC system with a Waters 2410 refractive index detector. Two 300 × 7.5 mm, 3 μm particle size ResiPore columns (Agilent Technologies) in series were used for sample separation. Samples were injected using THF as the eluent at a 1.0 ml/min flow rate. Standard curves were prepared using polystyrene standards ranging from 160 Da to 370 kDa. Shimadzu Lab Solutions software was used to calculate weight- (M_w) and number-average molecular weight (M_n) and the polydispersity index (PDI, M_w/M_n). Simvastatin monomer conversion (i.e., molecular weight growth of the poly(simvastatin) block) was determined as a function of time.

Asafo-Adjei T.A., Dziubla T.D, & Puleo D.A. (2014). Synthesis and Characterization of a Poly(ethylene glycol)-Poly(simvastatin) Diblock Copolymer. RSC advances, 4(102), 58287-58298.

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HPLC Polymer Molecular Weight Analysis

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A Shimadzu Prominence LC-20 AB HPLC system connected to a Waters 2410 refractive index detector was used to measure the number average (M_n) and weight average (M_w) molecular weights of polymeric prodrugs. Two Resipore columns in series (300 × 7.5 mm, 3 μm particle size; Agilent Technologies) were used for separation. Samples were dissolved in THF at 1 mg/mL and injected using THF as the mobile phase at a 1.0 mL/min flow rate at 40 ºC. The polystyrene standards used to calculate molecular weight ranged from 162 Da to 364 kDa.

Liyanage A.D., Chen A.J, & Puleo D.A. (2018). Biodegradable Simvastatin-Containing Polymeric Prodrugs with Improved Drug Release. ACS biomaterials science & engineering, 4(12), 4193-4199.

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Detailed Characterization of Polymers

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Gel permeation chromatography (GPC) measurements were performed on an Agilent 1260 LC system with two ResiPore columns (300 Â 7.5 mm, Agilent Technologies, Santa Clara, CA) in series at a flow rate of 1 mL min À1 at 70 1C, where DMF with 0.02 M LiBr was used as the mobile phase. The molecular weights were determined using a Wyatt miniDAWN TREOS multiangle light scattering detector and a Wyatt Optilab T-rEX differential refractive index detector. Liquid chromatrographymass spectrometry (LC-MS) analysis was performed using an Agilent 1260 Infinity LC system coupled with a 6130 quadrupole mass spectrometer. A mixture of 0.1% formic acid in water and MeCN was used as the mobile phase. NMR spectra were recorded on a Varian 400 MHz spectrometer. The residual undeuterated solvent peaks were used as references (7.27 ppm for CDCl 3 and 4.79 ppm for D 2 O).

Mahmad Rasid I., Do C., Holten-Andersen N, & Olsen B.D. (2021). Effect of sticker clustering on the dynamics of associative networks. Soft matter, 17(39).

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