Anti cortactin h 191

Synthetic AXT was purchased from Sigma-Aldrich, and dissolved in dimethyl sulfoxide (DMSO) to yield a 100 mM stock solution, and stored at −20 °C for future use. The primary antibodies (anti-E-cadherin (24E10), anti-Vimentin (5741) and anti-MMP2 (D204T)) were obtained from Cell Signaling. Anti-MYC (9E10) and anti-Cortactin (H-191) were obtained from Santa Cruz. Anti-β-actin (A5441) antibody was a product of Sigma Aldrich. Rhodamine Phalloidin (Thermo Fisher) was used for F-actin staining.

The following antibodies and working concentration were used in this study: anti-CTTNBP2 (A5, A7, and 9W, rabbit, homemade, 0.5 μg/ml) [5 (link), 20 (link)], anti-Myc tag (9B11, Cell Signaling Technology, 1/1000 for staining; 06-549, Millipore, 1 μg/ml), anti-HA tag (3F10, Roche, 0.5 μg/ml), anti-HA tag (Y-11, Santa Cruz Biotechnology, 0.5 μg/ml), anti-GFP (ab13970, Abcam, 0.5 μg/ml), anti-αtubulin (B-5-1-2, Sigma-Aldrich, 1 μg/ml), anti-acetyl tubulin (6-11B-1, Sigma-Aldrich, 1 μg/ml), anti-βactin (AC-74, Sigma-Aldrich, 1/1000), anti-cortactin (H-191, Santa Cruz Biotechnology, 0.5 μg/ml), anti-FOS (#2250, clone 9F6, Cell Signaling Technology, 1/200), anti-mouse HRP (NA931, GE Healthcare, 1/5000), anti-rabbit HRP (NA934, GE Healthcare, 1/5000), anti-chicken Alexa Fluor 488 (A-11039, Invitrogen, 1 μg/ml), anti-mouse Alexa Fluor 555 (A-21424, Invitrogen, 1 μg/ml), anti-rat Alexa Fluor 594 (A-21209, Invitrogen, 1 μg/ml), and anti-rabbit Alexa Fluor 647 (A-21244, Invitrogen, 2 μg/ml).