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Bim sc 374358

Manufactured by Santa Cruz Biotechnology

BIM (sc-374358) is a protein-specific antibody developed by Santa Cruz Biotechnology for research purposes. This antibody targets the BIM protein, which plays a role in apoptosis and cellular processes. The core function of this product is to provide researchers with a tool to detect and study the BIM protein in various biological samples and experimental settings.

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2 protocols using bim sc 374358

1

Nanoparticle Preparation and Antibody Profiling

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Silicon dioxide, in which 80% of particles exhibited a diameter of less than 5 μm, was purchased from Sigma® (S5631), processed via sedimentation according to Stokes’ law, subjected to acid hydrolysis, and heated overnight (200 °C for 16 h) [14 (link)]. Antibodies against ZC3H4 (2004 1–1-AP, rabbit), TrkB (13129-1-AP, rabbit), BIP (11578-1-AP, rabbit), CHOP (15204-1-AP, rabbit), ATF6α (66563-1-AP, mouse), ATG5 (60061-1-AP, mouse), BECN1 (11306-1-AP, rabbit), and LC3B (14600-1-AP, rabbit) were purchased from Proteintech, Inc. Antibodies against Akt (4691S, rabbit), p-Akt (4060S, rabbit), JNK (9258S, rabbit), p-JNK (4668S, rabbit) and IRE1α (3294, rabbit) were purchased from CST, Inc. Antibodies against GAPDH (sc-25778, rabbit), BIM (sc-374358) and NPNT (393033, rabbit) were purchased from Santa Cruz Biotechnology.
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2

Neuroinflammation and Neurogenesis Assays

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Antibodies against Bcl-2 interacting mediator of cell death (Bim;sc-374358) and B-cell lymphoma-extra large were obtained from Santa Cruz Biotechnology (Santa Cruz, CA); anti-parvalbumin (PV;P3088) and anti-glial fibrillary acidic protein (GFAP;G3893) antibodies were obtained from Sigma-Aldrich; bromodeoxyuridine (BrdU, NBP2-14890) was obtained from Novus; rabbit Anti-Goat IgG conjugated to Alexa Fluor® 488 (ab150141), anti-Doublecortin (DCX;ab153668) and anti-ionized calcium-binding adapter molecule 1 (Iba-1;ab5076) were obtained from Abcam; goat anti-mouse IgG conjugated to Alexa Fluor® 594 (A-11005), goat anti-chicken IgY conjutated to Alexa Fluor® 594 (A-11042), goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (A-11008) and anti-calcium/calmodulin-dependent protein kinase II (CaMKII;MA1-048) were obtained from Thermo Fisher Scientific. A rabbit polyclonal antibody against SGK1.1 was generated against a glutathione Stransferase (GST) fusion protein containing amino acids 41-80 from the SGK1.1-specific exon 2, expressed in E. coli and purified by affinity chromatography. KA (K0250) and M-current blocker XE991 (X2254) were obtained from Sigma Aldrich. SGK1 inhibitor EMD638683 was from MedChem Express (HY-15193A).
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