Acetonitrile 99.9

Acetonitrile 99.9% was of HPLC grade was obtained from Fisher Scientific (Lisbon, Portugal). Phenolic compound standards were obtained from Extrasynthese (Genay, France). Formic acid was obtained from Sigma-Aldrich (St. Louis, MO, USA). Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA). Fetal Bovine Serum (FBS), solutions of penicillin (100 IU/mL) and streptomycin (10 mg/mL), RPMI-1640 medium, trypsin-EDTA (ethylenediaminetetraAcetic acid), L-glutamine and Hank’s Balanced Salt Solution (HBSS) were purchased from Hyclone (Logan, USA). Acetic acid, sulforhodamine B (SRB), trypan blue, trichloroAcetic acid (TCA) and Tris-base were purchased from Sigma Chemical Co. (Saint Louis, USA). Silica gel 0.060–0.200 mm 60 A was obtained from Acros Organics (Geel, Belgium).

Acetonitrile 99.9%, n-hexane 95% and methanol 99.9% were purchased from Fisher Scientific (Shanghai, China). The fatty acids methyl ester (FAME) reference standard mixture 37 (standard 47885-U) was purchased from Sigma (St. Louis, MO, USA). Sugars standards [glucose, sorbitol and d(+)-trehalose and myo-inositol], phenolic standards (gallic, 4-hydroxybenzoic, 3,4-dihydroxybenzaldehyde, homogentisic, p-coumaric and o-coumaric acids) were purchased from Sigma (St. Louis, MO, USA) or Sigma-Aldrich Chem (shanghai, China). 1,1-Diphenyl-2-picryl-hydrazyl (DPPH), BHT, 3-(2-pyridyl)-5,6-bis (4-phenyl-sulfonic acid)-1,2,4-triazine (ferrozine), rutin and ascorbic acid were purchased from Sigma Chemical Co. BSTFA + TMCS (99:1) was purchased from Supelco. Folin–Ciocalteu’s phenol reagent was purchased from Beijing Solarbio reagent company (Beijing, China). Ferrous chloride, sodium salicylate, ferrous sulphate, hydrogen peroxide, potassium ferricyanide, trichloroacetic acid, disodium phosphate, sodium dihydrogen phosphate, ferric chloride, sodium carbonate, sodium hydroxide, aluminium nitrate, sodium nitrite, petroleum ether (60–90 °C), ethanol and methanol were all purchased from Beijing chemical plant (Beijing, China). All chemicals were of analytical grade. Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, USA).

Acetonitrile 99.9%, of high performance liquid chromatography (HPLC) grade, and sulphuric acid were acquired from Fisher Scientific (Lisbon, Portugal). Formic acid was acquired from Panreac (Barcelona, Spain). Sugar standards [D(−)-fructose, D(+)-glucose anhydrous and D(+)-sucrose], organic acid standards (malic acid, shikinic acid; oxalic acid and quinic acid), and trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) were acquired from Sigma Chemical Co. (Saint Louis, MO, USA). Phenolic compound standards (catechin, gallic acid, isorhamnetin 3-O-glucoside, kaempferol 3-O-glucoside, kaempferol 3-O-rutinoside, myricetin, quercetin 3-O-glucoside and quercetin 3-O-rutinoside) were purchased from Extrasynthese (Genay, France). 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was obtained from Alfa Aesar (Ward Hill, MA, USA). Water was treated by means of a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA).