Protein assay kit

Manufactured by Boster Bio

Sourced in China

The Protein Assay Kit is a laboratory instrument used to quantify the total protein concentration in a sample. It provides a standardized method for measuring the amount of protein present, allowing researchers to determine protein levels accurately and consistently.

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Lab products found in correlation

Wl00009b, by Wanlei (1 mentions) Ripa lysis buffer, by Boster Bio (1 mentions) Wbuls0500, by Merck Group (1 mentions) C3117, by Merck Group (1 mentions) Rabbit anti wnt3a, by Cell Signaling Technology (1 mentions) Rabbit anti β actin, by Cell Signaling Technology (1 mentions) Rabbit anti β catenin, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Supersignal west pico ecl solution, by Thermo Fisher Scientific (1 mentions) Ab85688, by Abcam (1 mentions) Chemiluminescence kit, by Merck Group (1 mentions) Bicinchoninic acid (bca), by Boster Bio (1 mentions) Ab192890, by Abcam (1 mentions) Horseradish peroxidase conjugated anti rabbit antibody, by Santa Cruz Biotechnology (1 mentions) Odyssey imaging system, by LI COR (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Ab184699, by Abcam (1 mentions) Cw0100, by CWBIO (1 mentions) Wbkls0500, by Merck Group (1 mentions) Pvdf membrane, by Merck Group (1 mentions)

7 protocols using protein assay kit

Quantitative Analysis of VEGFA Levels

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HUVECs with or without SCF were
lysed with RIPA lysate (Pro Tech) at hours 3, 6, and 9. Protein concentrations
were tested with a protein assay kit (Bosterbio). The total proteins
were separated by SDS-PAGE, transferred to a PVDF membrane (the membrane
was blocked with 5% non-fat milk), incubated with a vascular endothelial
growth factor A (VEGFA) (Wanleibio, WL00009b) primary antibody at
4 °C overnight, and then incubated with a secondary antibody
(ZSGBBI O, ZB2301) after washing. The signal of the immunoreactive
protein was visualized using a hypersensitive ECL chemiluminescence
reagent (Boster, AR1171) and then analyzed by using Image J software.

Mu X., Shi L., Pan S., He L., Niu Y, & Wang X. (2020). A Customized Self-Assembling Peptide Hydrogel-Wrapped Stem Cell Factor Targeting Pulp Regeneration Rich in Vascular-Like Structures. ACS Omega, 5(27), 16568-16574.

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Western Blot Analysis of Wnt3a and β-Catenin

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Protein samples were prepared from brain tissues and PC12 cells using RIPA lysis buffer (AR0105; Boster, Wuhan, China) and quantified with a Protein Assay kit (AR0146; Boster). In order to detect the levels of protein expression, protein samples were separated by 10% SDS-PAGE gel and then transferred onto a PVDF membrane (C3117; Millipore, MA, USA). Following sealed with 5% skimmed milk powder at room temperature for 1 h, the membranes were incubated with rabbit anti-Wnt3a (#2721), rabbit anti-β-catenin (#8480), and rabbit anti-β-actin (#4970; 1:1000; Cell Signaling Technology, MA, USA) at 4°C overnight and then incubated with goat anti-rabbit IgG at room temperature for 1 h. β-actin was used as inner loading control. Protein bands were visualized using an ECL chemiluminescence kit (WBULS0500; EMD Millipore).

Wang Y., Wang Q., Li J., Lu G, & Liu Z. (2019). Glutamine Improves Oxidative Stress through the Wnt3a/β-Catenin Signaling Pathway in Alzheimer's Disease In Vitro and In Vivo. BioMed Research International, 2019, 4690280.

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Quantitative Western Blot Analysis of Prolactinoma

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Prolactinoma specimens and cells were lysed in lysis buffer for 30 min on ice. The total protein was quantified using a Protein Assay kit (Boster, Inc. cat. no. AR0106). Equal amounts of protein (30 µg) were separated on 10-15% SDS-PAGE gels and transferred onto nitrocellulose membranes. After blocking with 5% milk in TBST for 2 h at room temperature, the membranes were incubated with the aforementioned primary antibodies (1:1,000) at 4 °C overnight. The membranes were then washed in TBST buffer and incubated with HRP-conjugated secondary antibodies (1:3000) for 2 h at room temperature. After a final wash step, the membranes where developed using SuperSignal West Pico ECL solution (Thermo Fisher Scientific, Inc. cat. no. 34580) and the protein bands were detected and visualized using the FluorChem™ E imaging system (ProteinSimple).

Xiao Z., Yang X., Zhang K., Liu Z., Shao Z., Song C., Wang X, & Li Z. (2020). Estrogen receptor α/prolactin receptor bilateral crosstalk promotes bromocriptine resistance in prolactinomas. International Journal of Medical Sciences, 17(18), 3174-3189.

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Quantifying Mel-18 and Bmi-1 in Esophageal Cancer

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Human esophageal cancer tissues and adjacent noncancerous mucosal tissue were homogenized and centrifuged at 12 000g for 30 minutes at 4°C. The protein concentrations were determined using the BCA (Bicinchonininc Acid) Protein Assay Kit (BOSTER, Wuhan, China). Thirty micrograms of total protein was separated by 10% SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis), transferred to a PVDF (Polyvinylidene Fluoride) membrane, and blocked in 5% milk. The membranes were incubated with rabbit polyclonal antibody for Mel-18 (1:500 dilution, bs-9673 R, Bioss, Beijing, China) and rabbit polyclonal antibody for Bmi-1 (1:8000 dilution, ab85688, ABCAm) or GAPDH (Glyceraldehyde Phosphate Dehydrogenase) (1:6000 dilution, China) at 4°C overnight. The secondary antibody (1:8000 dilution, China) was incubated with the membrane for 1 hour at room temperature. Finally, the immunoreactive protein bands were visualized with a chemiluminescence kit (Millipore, MA, USA).

Ji H., Cao M., Ren K., Sun N., Wang W., Zhu Q., Zang Q, & Jiang Z. (2017). Expression and Clinicopathological Significance of Mel-18 and Bmi-1 in Esophageal Squamous Cell Carcinoma. Technology in Cancer Research & Treatment, 16(6), 828-834.

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Quantifying Angiogenic Potential of Nerve Grafts

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Four
regenerated nerve
grafts (CST, DPSCs, SCF + DPSCs, and Autograft) were lysed with RIPA
lysate (ProTech) 12 weeks after operation. Then, the protein concentration
was detected using a protein assay kit (Bosterbio). Next, the total
protein was separated via sodium dodecyl sulfate-polyacrylamide gel
electrophoresis (SDS-PAGE) and transferred to the polyvinylidene fluoride
(PVDF) membrane preblocked with 5% non-fat milk. Then, the PVDF membrane
was incubated with anti-CD31 monoclonal primary antibody (GeneTex,
JC70A, 1:100) at 4 °C overnight and incubated with secondary
antibody (Invitrogen, 31430, 1:5000) for 1 h. The signals of CD31
were visualized using the hypersensitive ECL chemiluminescence reagent
(Boster, AR1171), and the result was analyzed using the Image J software
for the relative CD31 protein expression level.

Mu X., Liu H., Yang S., Li Y., Xiang L., Hu M, & Wang X. (2022). Chitosan Tubes Inoculated with Dental Pulp Stem Cells and Stem Cell Factor Enhance Facial Nerve-Vascularized Regeneration in Rabbits. ACS Omega, 7(22), 18509-18520.

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Protein Expression Analysis in SH-SY5Y Cells

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SH-SY5Y cells were lysed with RIPA Lysis Buffer (P0013B; Beyotime, China). The concentration of total protein was measured by protein assay kit (AR0146; Boster, China). The protein samples were separated using 10% or 12% SDS-PAGE gel and blotted to PVDF membranes. Subsequently, the PVDF membranes were incubated overnight at 4℃ in primary antibodies as follows: p-ERK (1:1000; #4370, CST, U.S.), ERK (1:1000; ab184699, Abcam, U.K.), p-Drp1S616 (1:1000; #3455, CST, U.S.), Drp1 (1:1000; #8570S, CST, U.S.), Mfn2 (1:1000; #9482S, CST, U.S.), Mfn1 (1:1000; #14,739, CST, U.S.), Opa1 (1:1000; #67589S, CST, U.S.), LC3B (1:2000; ab192890, Abcam, U.K.), Beclin1 (1:2000; 11,306, Proteintech, China), p62 (1:1000; #5114, CST, U.S.), GAPDH (1:1000; #5174, CST, U.S.). After being washed with Tris-buffered saline, the membranes were incubated in anti-rabbit horseradish peroxidase-conjugated antibodies (1:10000; Santa Cruz Biotechnology, U.S.) for 1 h at room temperature. The target protein bands were detected with Odyssey imaging system (LI-COR, U.S.).

Yuan Z.L., Mo Y.Z., Li D.L., Xie L, & Chen M.H. (2023). Inhibition of ERK downregulates autophagy via mitigating mitochondrial fragmentation to protect SH-SY5Y cells from OGD/R injury. Cell Communication and Signaling : CCS, 21, 204.

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Western Blot Analysis of Hippocampal and Prefrontal Cortex Proteins

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Brains were quickly removed immediately after the last working memory test (retrieval). HIP and PFC tissues were dissected in cold artificial cerebrospinal fluid. These tissues were homogenized in a protein extraction buffer containing protease and phosphatase inhibitors. Protein concentration was measured using a Protein Assay kit (Boster, China). Proteins were separated on 10 % SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore). After being blocked in 5 % milk for 1 h at room temperature, membranes were incubated overnight at 4 °C with the following primary antibodies: anti-HCN1 (1:1,000, NBP1-20250, Novus), anti-p44/42 MAPK (Erk1/2) (1:1,000, 4695S, Cell Signaling), antiphospho-p44/42 MAPK (Thr202/Tyr204) (1:1,000, 9101S, Cell Signaling), or GAPDH (1:5,000, cw0100, Cwbiotech). After incubation with horseradish peroxidase conjugated secondary antibodies (1:5,000; Proteintech Group Inc., China), bands were developed with chemiluminescent substrate (WBKLS0500, Millipore). Immunoreactive signals were quantified by NIH ImageJ software.

Zhou M., Lin K., Si Y., Ru Q., Chen L., Xiao H, & Li C. (2019). Downregulation of HCN1 channels in hippocampus and prefrontal cortex in methamphetamine re-exposed mice with enhanced working memory. Physiological research, 68(1).

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