Butyl gallate

All chemicals were of the highest purity available and used as received. Gallic acid monohydrate (98.0% pure) and gallates (propyl gallate, PG, butyl gallate, BG, octyl gallate, OG, and lauryl gallate, LG, all 99.0% pure) were purchased from Sigma–Aldrich. 2–Hydroxypropyl–β–cyclodextrin (HP–β–CD) and methyl–β–cyclodextrin (M–β–CD) were purchased form Cyclolab LTD and acetonitrile was received from (ACN, HLPC grade) Merck. The Folin-Ciocalteu’s reagent was obtained by VWR Chemical. Sodium carbonate and 2,2–diphenyl–1–pycrilhydrazyl (DPPH^●) were purchased from Sigma–Aldrich. Milli-Q grade water (conductivity < 0.1 mS cm⁻¹) was employed in the preparation of aqueous solutions. Citric acid and sodium citrate (both from Acros Organics, 99% pure) 0.04 M, pH 3.65, was used as buffer solution.

All chemicals used were of analytical reagent grade. All reagents were purchased from Sigma, Aldrich: Ascorbic acid, Acetic acid, acetylcholinesterase (AChE), acetylthiocholine (ACTHI) acetonitrile (ACN), N, O-bis (trimethylsilyl) trifluoroacetamide (BSTFA), cyclohexane (CYH), dichloromethane (DCM), Dulbecco's modified eagle medium (DMEM), dimethyl sulfoxide (DMSO), 1-1-diphenyl-2-picryl hydrazyl (DPPH), 5, 5-dithiobis-2-nitrobenzoic acid (DTNB), Folin-Ciocalteu reagent (2 N), methanol (MeOH), human colon cancer cells (HCT116), human breast cancer cells (MCF-7), 3- [4, 5-dimethylthiazol-2yl]-2, 5-diphenyl tetrazolium bromide (MTT), Roswell Park Memorial Institute (RPMI), Chlorotrimethylsilane (TMCS), and tetrahydrofuran (THF).
The analytical standards used for the identification and quantification of the principal phenolic compounds found in the extracts: 3-amino-4-hydroxybenzoic acid, Gallic acid, 3, 4-dihydroxy-5 methoxybenzoic acid, 7-hydroxycoumarin-3-carboxylic acid, rutin hydrate, Butyl gallate, 4-hydroxytamoxifen, cardamonin, phenoxodiol, pinostilbene hydrate, 3-benzyloxy-4, 5-dihydroxy-benzoic acid methyl ester, ethyl trans-2-hydroxycinnamate, 4′, 5-dihydroxy-7-methoxyflavone, pinosylvin monomethyl ether, 3, 6, 3′-trimethoxyflavone, shikonin, 5-hydroxy-3′-methoxyflavone, and 3′-hydroxy-b-naphthoflavone were also purchased from Sigma, Aldrich.