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Singlet oxygen sensor green (sosg)

Manufactured by Meilun
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Sourced in China

The SOSG is a laboratory instrument used for measuring the optical properties of solutions. It functions by detecting and analyzing the scattering of light as it passes through a liquid sample. The core purpose of the SOSG is to provide precise data on the light scattering characteristics of the tested solution.

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Lab products found in correlation

Dapi solution, by Solarbio (1 mentions) Fetal bovine serum (fbs), by Procell (1 mentions) Balb c mice, by Charles River Laboratories (1 mentions) Metformin, by Maclin Biochemical Technology (1 mentions) Rpmi 1640, by Procell (1 mentions) Cetyltrimethylammonium bromide ctab, by Merck Group (1 mentions) Tunel apoptosis assay kit, by Solarbio (1 mentions) Ir780, by Merck Group (1 mentions) 2 7 dichlorodihydrofluorescein diacetate dcfh da, by Merck Group (1 mentions) Anti cd31 rabbit polyclonal antibody, by Abcam (1 mentions) Annexin 5 fitc 7aad apoptosis kit, by BD (1 mentions) Carry eclipse fluorescence spectrophotometer, by Agilent Technologies (1 mentions) Nadph, by J&K Scientific (1 mentions) Tetrahydrofuran, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Zhejiang Tianhang Biotechnology (1 mentions) 1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc, by Merck Group (1 mentions) N hydroxysulfosuccinimide sodium salt sulfo nhs, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

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Singlet oxygen sensor green reagent (SOSG) (2 citations)

6 protocols using singlet oxygen sensor green (sosg)

1

Multifunctional Nanoparticle Tumor Therapy

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NIR dye CyI (MW 776.5) was prepared in our laboratory. Egg yolk lecithin (PC-98T) was acquired from AVT Pharmaceutical Tech Co. (Shanghai, China). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000) was purchased from Ruixi Bio-tech Co. (Xi’an, China). Metformin was purchased from Macklin. Singlet Oxygen Sensor Green (SOSG) was purchased from Meilunbio. DAPI solution, Methyl thiazolyl-tetrazolium (MTT), Annexin V-FITC/PI apoptosis staining kit were purchased from Solarbio (Beijing, China).
Mouse colon cancer cells (CT26) were purchased from Wuhan Procell Life Science & Technology. Co. Ltd. (Wuhan, China). Cells were cultured in Roswell Park Memorial Institute-1640 (RPMI-1640, Procell, USA) with 10% fetal bovine serum (Procell, USA) and 1% penicillin-streptomycin (Hyclone, USA). BALB/c mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China).
Zhao Y., Lv B., Xue G., Sun Y, & Cao J. (2023). Smart Nanosystem-Mediated Inhibition of Mitochondrial Respiration for Enhanced Phototherapy-Induced Antitumor Immunity. International Journal of Nanomedicine, 18, 3443-3457.
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2

Multimodal Evaluation of Nanocarrier-Mediated Therapy

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IR-780, 2′,7′-dichloro-dihydrofluorescein diacetate (DCFH-DA) and cetyl trimethyl ammonium bromide (CTAB) were purchased from Sigma-Aldrich (St Louis, MO, USA). DOX, 4′,6′-diamidine-2′-phenylindole (DAPI) and singlet oxygen sensor green (SOSG) were bought from Meilun Biotech (Dalian, China). Sodium salicylate (NaSal) and tetraethyl orthosilicate (TEOS) were purchased from Heowns Biochemical Technology (Tianjin, China). Annexin-V-FITC/7AAD apoptosis kit was bought from (BD Biosciences, San Jose, USA). LIVE/DEAD cell imaging kit and monoclonal antibodies against CD11a-FITC and CD62P-APC were provided by eBioscience (San Diego, CA, USA). Coomassie brilliant blue methylthiazolyldiphenyl-tetrazolium bromide (MTT) and TUNEL apoptosis assay kit were sourced from SolarbioScience & Technology (Beijing, China). Rabbit anti-CD31 polyclonal antibody was purchased from Abcam (Cambridge, MA, USA).
Zhang T., Liu H., Li L., Guo Z., Song J., Yang X., Wan G., Li R, & Wang Y. (2021). Leukocyte/platelet hybrid membrane-camouflaged dendritic large pore mesoporous silica nanoparticles co-loaded with photo/chemotherapeutic agents for triple negative breast cancer combination treatment. Bioactive Materials, 6(11), 3865-3878.
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3

Fluorescence-based Enzymatic Assay

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Na2S2O4 was purchased from Energy Chemical Co. Ltd. (Shanghai, China). SOSG was purchased from Dalian Meilun Biotechnology Co. Ltd. (Dalian, China). NTR was obtained from Sigma-Aldrich Co. Ltd. (Shanghai, China). NADPH was purchased from J&K Scientific, Co. Ltd. (Beijing, China). All UV-Vis spectra were recorded on a TU-1901 spectrophotometer (PuXi Science and Technology Development Co., Ltd., Beijing, China). The fluorescence analyses were performed on a Carry Eclipse fluorescence spectrophotometer (Agilent, Santa Clara, CA, USA). A Laser (660 nm) was obtained from Changchun Femtosecond Technology Co., Ltd., (Changchun, China).
Ding S., Yang M., Lv J., Li H., Wei G., Gao J, & Yuan Z. (2022). Novel Lysosome-Targeting Fluorescence Off-On Photosensitizer for Near-Infrared Hypoxia Imaging and Photodynamic Therapy In Vitro and In Vivo. Molecules, 27(11), 3457.
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4

Singlet Oxygen Detection in Cu-Cy Nanoparticles

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A singlet oxygen sensor green reagent (SOSG) (Ex = 505 nm, Em = 525 nm) was used to detect the 1O2 in the aqueous solution. SOSG (100 μg, Meilunbio, China) was dissolved in methanol (33 μL) to make a 5 mM stock solution. The SOSG stock solution was diluted with PBS to a working solution of 50 μM. 100 μL of Cu-Cy nanoparticles (10, 20, 40, and 80 μg/mL) were loaded into four wells of a 96-well plate, and 10 μL of SOSG stock solution was added to each well. After mixing, MW (20 W, 3 min) was irradiated, and the fluorescence of SOSG was measured with a microplate reader immediately after each microwave treatment.
Zhou H., Liu Z., Zhang Z., Pandey N.K., Amador E., Nguyen W., Chudal L., Xiong L., Chen W, & Wen Y. (2022). Copper-cysteamine nanoparticle-mediated microwave dynamic therapy improves cancer treatment with induction of ferroptosis. Bioactive Materials, 24, 322-330.
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5

Multimodal Sensing Platform Development

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Reagents include MPTMS (95%), tetrahydrofuran (THF), ammonium hydroxide aqueous solution (28%), 1-ethyl-3-(3-dimethylamino propyl) carbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide sodium salt (Sulfo-NHS), catalase (CAT), and LOx were purchased from Sigma-Aldrich. Indocyanine Green (ICG, 98%) was purchased from Hwrk Chem. Acid phosphatase (AP), 1,2-dedecanediol was purchased from TCI (Shanghai) Development Co., Ltd., and 2-[4-(2-hydroxyethyl) piperazin-1-yl] ethanesulfonic acid (HEPES), tris(hydroxymethyl) aminoethane (Tris), and Fmoc-Tyr (H2PO3)-OH were purchased from J&K Scientific (China). SOSG was purchased from Dalian Meilun Biotechnology Co., Ltd. Methylene blue (MB), hydrogen peroxide (H2O2), glutathione (GSH), oleic acid, oleylamine, biphenyl ether, n-hexane, 1,10-phenanthroline monohydrate, and lactate (90% in water) were purchased from Aladdin. The 5,5-dimethyl-1-pyrroline N-oxide (DMPO) was purchased from Nantong Feiyu Biological Technology Co., Ltd. FBS was purchased from Zhejiang Tianhang Biotechnology Co., Ltd, China. DHE and cell culture products were purchased from Keygentec (Nanjing, China). All the reagents, except to that were not pointed out specially, were analytical grade, and ultra-pure water was used in the preparation of all samples.
Qin X., Wu C., Niu D., Qin L., Wang X., Wang Q, & Li Y. (2021). Peroxisome inspired hybrid enzyme nanogels for chemodynamic and photodynamic therapy. Nature Communications, 12, 5243.
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6

Targeted Nanoparticle Delivery System

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Ce6, DiR, BDP, PPa, Hy, and SOSG were obtained from Meilun Biotech Co. Ltd, China. The anti-CD47 antibody was acquired from Biosynthesis Biotechnology Inc, China. Shanghai Advanced Vehicle Technology Co. Ltd. provided DSPE-PEG2k. Vessels for cell culture were supplied by Wuxi NEST Biotechnology Co., Ltd, China. Fetal bovine serum (FBS) and cell culture media were provided from GIBCO, Invitrogen Corp. The anti-CD31 antibody was purchased from Beijing Solarbio Science & Technology Co., Ltd, China. The solvents and reagents used in this study were all of the analytical grades.
Zhang X., Xiong J., Wang K., Yu H., Sun B., Ye H., Zhao Z., Wang N., Wang Y., Zhang S., Zhao W., Zhang H., He Z., Luo C, & Sun J. (2021). Erythrocyte membrane-camouflaged carrier-free nanoassembly of FRET photosensitizer pairs with high therapeutic efficiency and high security for programmed cancer synergistic phototherapy. Bioactive Materials, 6(8), 2291-2302.
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