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Anti camkiiδ

Manufactured by Santa Cruz Biotechnology
Sourced in United States

Anti-CaMKIIδ is a primary antibody that specifically binds to the CaMKIIδ protein. CaMKIIδ is a calcium/calmodulin-dependent protein kinase that plays a crucial role in various cellular processes. This antibody can be used for the detection and analysis of CaMKIIδ expression in different experimental systems.

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2 protocols using anti camkiiδ

1

Immunoblot and Immunohistochemistry Protocols

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We used anti-hemagglutinin (HA) antibody (3F10) conjugated with peroxidase (Roche, Mannheim, Germany), anti-CaMKI (Santa Cruz Biotechnology, Santa Cruz, CA), anti-CaMKIIδ (Santa Cruz Biotechnology), anti-transferrin receptor (Zymed, South San Francisco, CA), and anti-NET antibody (NET17-1, Mab Technologies, Atlanta, GA) for immunoblots. Rabbit polyclonal NET antibodies 43408 raised against an epitope at the extracellular loop of NET (Savchenko et al., 2003 (link)) and monoclonal anti-β tubulin (Sigma, St. Louis, MO) antibodies were used for immunohistochemistry. siRNAs for CaMKI and CaMKIIδ (SMARTpool™) were purchased from Dharmacon (Lafayette, CO). cDNA constructs for human NET and its mutants (NET Δ28–47, NET T30A, NET T30E) were N-terminally tagged by inserting HA-tag (YPYDVPDYA) between the first and second amino acids.
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2

Cardiac Protein Expression Analysis

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Anti-CaMKIIδ (Item No. sc-100362) was purchased from Santa Cruz (USA), anti-CaMKII (phospho T287) (Item No. ab182647), anti-β Tubulin (Item No. ab6046), anti-cardiac troponin T (Item No. ab209813) were purchased from Abcam (Cambridge, UK), Tropomyosin-1/3 (Item No. D17B8) were purchased from Cell Signaling Technology (Beverly, MA, USA), Anti-MYOM2 (Item No. A20526) was purchased from ABclonal (Wuhan, China). KN-93 and KN-92 were obtained from Selleck (Houston, TX, United States).
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