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Th 5 thermometer

Manufactured by Physitemp

The TH-5 thermometer is a compact and versatile device designed for measuring temperature. It features a digital display and is capable of taking measurements in degrees Celsius or Fahrenheit. The TH-5 is powered by a battery and is suitable for a variety of applications that require accurate temperature readings.

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13 protocols using th 5 thermometer

1

Rectal Temperature Measurement Protocol

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Internal body temperature (°C) was measured between 6 and 8 a.m. at various time points during the study with a Thermalert TH-5 thermometer with a flexible rectal probe (Physitemp Instruments, Clifton, NJ).
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2

Measuring Tissue Temperature in Mice

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Tissue temperature in the interscapular BAT and the skeletal muscle was recorded using the type T thermocouple probes and recorded by using the TC-2000 Meter (Sable Systems International), according to our protocol3 (link). Core body temperature was monitored using a TH-5 thermometer (Physitemp) by recording the rectal temperature of the mice. For core-body temperature recording experiment in Fig. 5i, both control (Ppargflox/flox) and PpargMyoD KO (Myod-CreERT2;Ppargflox/flox) mice were pre-treated with β-blocker and tamoxifen at room temperature for 5 days and acclimated to 15°C for additional 5 days. Wild-type mice did not receive pre-β-blocker-treatment. Subsequently, all the mice were acutely treated with β-blocker and exposed to 10°C.
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3

Core and Tissue Temperature Monitoring in Transgenic Mice

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For core-body temperature recording experiments, rectal temperature of BckdhaUCP1 KO and Slc25a44 KD mice was monitored using a TH-5 thermometer (Physitemp) up to 14 hours after cold exposure. For tissue temperature recording, mice under anesthesia were implanted with type T thermocouple probes in the interscapular BAT, inguinal WAT, liver, and skeletal muscle, according to the method that was described previously 33 . Tissue temperature was recorded by TC-2000 Meter (Sable Systems International). When tissue temperature was stable, mice were intraperitoneally administered norepinephrine at a dose of 1 mg per kg body weight to induce non-shivering thermogenesis.
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4

Monitoring Tissue Temperatures in Mice

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The rectal temperature of mice was monitored by a TH-5 thermometer (Physitemp). For the recording of the tissue, mice under anesthesia were implanted with type T thermocouple probes in the interscapular BAT, inguinal WAT, and skeletal muscle, according to the previous study 12 . The temperature of each tissue was recorded by TC-2000 Meter (Sable Systems International). When the tissue temperature keeps stable, mice were treated with norepinephrine at a dose of 3 mg per kg body weight to induce non-shivering thermogenesis.
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5

Measuring Tissue Temperature in Mice

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Tissue temperature in the interscapular BAT and the skeletal muscle was recorded using the type T thermocouple probes and recorded by using the TC-2000 Meter (Sable Systems International), according to our protocol3 (link). Core body temperature was monitored using a TH-5 thermometer (Physitemp) by recording the rectal temperature of the mice. For core-body temperature recording experiment in Fig. 5i, both control (Ppargflox/flox) and PpargMyoD KO (Myod-CreERT2;Ppargflox/flox) mice were pre-treated with β-blocker and tamoxifen at room temperature for 5 days and acclimated to 15°C for additional 5 days. Wild-type mice did not receive pre-β-blocker-treatment. Subsequently, all the mice were acutely treated with β-blocker and exposed to 10°C.
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6

Monitoring Tissue Temperatures in Mice

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The rectal temperature of mice was monitored by a TH-5 thermometer (Physitemp). For the recording of the tissue, mice under anesthesia were implanted with type T thermocouple probes in the interscapular BAT, inguinal WAT, and skeletal muscle, according to the previous study 12 . The temperature of each tissue was recorded by TC-2000 Meter (Sable Systems International). When the tissue temperature keeps stable, mice were treated with norepinephrine at a dose of 3 mg per kg body weight to induce non-shivering thermogenesis.
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7

Core and Tissue Temperature Monitoring in Transgenic Mice

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For core-body temperature recording experiments, rectal temperature of BckdhaUCP1 KO and Slc25a44 KD mice was monitored using a TH-5 thermometer (Physitemp) up to 14 hours after cold exposure. For tissue temperature recording, mice under anesthesia were implanted with type T thermocouple probes in the interscapular BAT, inguinal WAT, liver, and skeletal muscle, according to the method that was described previously 33 . Tissue temperature was recorded by TC-2000 Meter (Sable Systems International). When tissue temperature was stable, mice were intraperitoneally administered norepinephrine at a dose of 1 mg per kg body weight to induce non-shivering thermogenesis.
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8

Thermoregulation in Adipo-Cul2/Appbp2-KO Mice

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Adipo-Cul2-KO mice, Adipo-Appbp2-KO mice and their respective littermate control mice were kept on a regular chow diet. Mice were acclimatized to 30 °C for 11 days and subsequently exposed to 8 °C for 6 h. The rectal temperatures of mice were monitored every 1 h using the TH-5 thermometer (Physitemp).
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9

Cold Exposure and Thermoregulation

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The mice were maintained in a climate chamber (Columbus Instruments, Ohio) at 5 °C with free access to water but not food. The rectal temperature was measured with a TH5 thermometer (Physitemp, Clifton, NJ) at the indicated time after exposure to the cold.
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10

Measuring Tissue Temperatures in Mice

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Rectal temperature of the mice was monitored using a TH-5 thermometer (Physitemp). For tissue temperature recording, mice under anesthesia were implanted with the type T thermocouple probes at interscapular BAT, inguinal WAT, and skeletal muscle. Temperature in skeletal muscle on the back was record according to the previous study 51 . The temperature at each tissue was recorded by TC-2000 Meter (Sable Systems International). Core body temperature was maintained at 37°C or above. When tissue temperature reached stable, animals were treated with norepinephrine at a dose of 1 mg/kg.
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