Zinc acetate zn ch3coo 2 2h2o

The UPR PP-250 provided by Poliformas Plasticas Mexico was used as a matrix. This liquid resin is composed of a copolymer of maleic acid (30% mol) and isophthalic acid (30% mol) with propanediol (40% mol), and styrene (20 wt % styrene) with a small fraction of polymerization inhibitor. Since the PP-250 resin is hardened via a radical process, cobalt octoate used as an accelerator and the methylethylketone peroxide initiator solution were added to the mixture.
Analytical grade zinc acetate (Zn(CH₃COO)₂·2H₂O 99.8%) and oxalic acid (C₂H₂O₄·2H₂O, 99.8%) from Sigma-Aldrich were used without further purification.

Escherichia coli (KCTC1682), Pseudomonas aeruginosa PAO1 (KCTC1637), Listeria monocytogenes (KCTC3569), and Staphylococcus aureus (KCTC 1916) were purchased from the Korean Collection for Type Cultures (KCTC, Daejeon, Korea). Klebsiella pneumoniae (ATCC4352) was purchased from American Type Culture Collection (ATCC). Streptococcus mutans (KCCM 40105) and Candida albicans (KCCM11282) were purchased from the Korean Culture Center of Microorganisms (KCCM; Seodaemun-gu, Seoul, South Korea). Tryptic soy broth (TSB; Difco Laboratory Inc., Detroit, MI, USA) was used to grow P. aeruginosa, S. aureus, E. coli, L. monocytogenes, and Vibrio vulnificus. For culturing C. albicans, the potato dextrose broth (PDB) containing glucose (5 %) was used, whereas for culturing the S. mutans, the brain heart infusion (BHI) broth (Difco Laboratory Inc., Detroit, MI, USA) was used. A deMan Rogosa Sharpe Medium (MRS; Difco Laboratory Inc., Detroit, MI, USA) was used to cultivate LAB. Gold (III) chloride trihydrate (CAS# 16961-25-4; purity 99%) and zinc acetate (Zn(CH₃-COO)₂·2H₂O) (CAS # 5970-45-6) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). The temperature for the growth of all microbes was 37 °C.

Pseudomonas aeruginosa PAO1 (KCTC 1637) was purchased from the Korean Collection for Type Cultures (KCTC, Daejeon, Korea). Tryptic soy broth (TSB; Difco Laboratory Inc., Detroit, MI, USA) was utilized as the culture medium for the growth of P. aeruginosa. Gold (III) chloride trihydrate (CAS # 16961-25-4; purity 99%), phloroglucinol (CAS # 108-73-6; purity ≥ 99%), zinc acetate (Zn(CH₃-COO)₂.2H₂O) (CAS # 5970-45-6), and commercial ZnONPs (CAS # 1314-13-2; purity > 97% and particle size < 50 nm) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). The composition of minimal salt medium (MSM), per liter, includes Na₂HPO₄ (4.0 g), KH₂PO₄ (2.0 g), (NH₄)₂SO₄ (0.8 g), MgSO₄ (0.8 g), and 1 mL trace element solution (TES) [67 (link)]. The composition of the TES, per liter, includes (NH₄)₆Mo7O₂₄.4H₂O (0.05 g), Al(OH)₃ (0.1 g), BaCl₂ (0.05 g), CoCl₂.6H₂O (0.1 g), H₃BO₃ (0.5 g), KI (0.05 g), LiCl (0.05 g), MnSO₄.4H₂O (0.08 g), NiSO₄.6H₂O (0.1 g), SnCl₂ (0.05 g), and ZnSO₄.7H₂O (0.1 g). The pH of MSM was 7.2.