Blood samples were taken from the hearts of the mice 8 and 12 weeks after surgery. Coagulated at room temperature 10–20 min, centrifugated 20 min at the speed of 2000–3000 r.p.m. to remove supernatant, If precipitation appeared, centrifugated again. To determine the concentrations of Estrogen in the serum, Estrogen BPA Environmental ELISA kits (abcam, cat. no. ab175820) were used according to the manufacturer's protocol.The absorbance was measured using a Multiskan MS 352 microplate reader (Thermo Fisher Scientific) at a wavelength of 450 nm. All the samples were thawed once and assayed in triplicate.
Multiskan ms 352
Manufactured by Thermo Fisher Scientific
The Multiskan MS 352 is a multi-channel microplate reader designed for absorbance measurements in 96-well microplates. It is capable of performing a variety of assays, including enzyme-linked immunosorbent assays (ELISA), colorimetric assays, and cell-based assays.
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Lab products found in correlation
2 protocols using multiskan ms 352
1
Estrogen Quantification in Mouse Serum
2
Serum TNF-α and IL-6 Quantification
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After the blood samples were allowed to stand for 4 to 5 h, serum was obtained after centrifugation at 3,000 × g for 5 min at 4°C. To determine the concentrations of TNF-α and IL-6 in the serum, TNF-α and IL-6 ELISA kits were used according to the manufacturer's protocol. The absorbance was measured using a Multiskan MS 352 microplate reader (Thermo Fisher Scientific, Inc.) at a wavelength of 450 nm. All the samples were thawed once and assayed in triplicate.
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