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Nod scid il2rγc

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The NOD-scid-IL2Rγc−/− mouse is a strain of immunodeficient mice that lack mature T cells, B cells, and natural killer cells. This mouse is commonly used in biomedical research as a model for studying human diseases and testing new therapies.

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Lab products found in correlation

C57bl 6j, by Jackson ImmunoResearch (2 mentions) Balb cj, by Jackson ImmunoResearch (2 mentions) Interleukin 2 (il 2), by R&D Systems (1 mentions) Collagenase, by STEMCELL (1 mentions) C57bl 6 b6, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

NOD/SCID (81 citations) NOD/SCID/IL2Rγc−/− (NSG) mice (4 citations) NOD-SCID IL2Rγc null mice (3 citations) NOD-SCID-IL2Rγc–/– mice (2 citations) NOD/SCID/IL2Rγc−/− (NSG), (2 citations) NOD/SCID/IL2Rγc-deficient (NSG) mice (2 citations)

6 protocols using nod scid il2rγc

1

NOD-scid IL2Rγc−/− Mouse Maintenance

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NOD-scid-IL2Rγc−/− (Jackson Laboratory) mice were bred and maintained in the Stem Cell Unit animal barrier facility at McMaster University. All procedures received the approval of the Animal Research Ethics Board at McMaster University.
Rentas S., Holzapfel N., Belew M.S., Pratt G., Voisin V., Wilhelm B.T., Bader G.D., Yeo G.W, & Hope K.J. (2016). Musashi-2 Attenuates AHR Signaling to Expand Human Hematopoietic Stem Cells. Nature, 532(7600), 508-511.
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2

Adoptive transfer of TCR-engineered CD8+ T-cells in NOD/SCID/IL-2Rγc−/− melanoma model

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Seven to 8wk old male NOD/SCID/IL-2Rγc−/− (Jackson Immunoresearch) mice were inoculated subcutaneously with 1.4 × 106 AAD+ SLM2 melanoma cells. Three x106 human CD8 T-cells expressing either pIRS-2- or pCDC25b-specific TCR, or 1.5×106 of both populations, were adoptively transferred 3d later. An additional 1.5 × 106 T-cells were given 4d later. All mice received 1500 CU of IL-2 (R & D Systems) i.p. every other day for 10d. Tumor size was measured every 2–3d with a digital caliper, and calculated as LxW (mm2). Animals were considered tumor free until the evaluation day when the tumor size was measurable (>30 mm2). Non-transfected human CD8 T-cells from both donors utilized did not recognize SLM2 melanoma in vitro (data not shown).
Zarling A.L., Obeng R.C., Desch A.N., Pinczewski J., Cummings K.L., Deacon D.H., Conaway M., Slingluff CL J.r, & Engelhard V.H. (2014). MHC-restricted phosphopeptides derived from Insulin receptor substrate-2 and CDC25b offer broad-based immunotherapeutic agents for cancer. Cancer research, 74(23), 6784-6795.
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3

NOD-scid IL2Rγc−/− Mouse Maintenance

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NOD-scid-IL2Rγc−/− (Jackson Laboratory) mice were bred and maintained in the Stem Cell Unit animal barrier facility at McMaster University. All procedures received the approval of the Animal Research Ethics Board at McMaster University.
Rentas S., Holzapfel N., Belew M.S., Pratt G., Voisin V., Wilhelm B.T., Bader G.D., Yeo G.W, & Hope K.J. (2016). Musashi-2 Attenuates AHR Signaling to Expand Human Hematopoietic Stem Cells. Nature, 532(7600), 508-511.
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4

Pre-HSC and T Progenitor Transplantation

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C57BL/6 (B6), their congenic BoyJ, and NOD/SCID/IL2Rγc–/– (NSG) mice were purchased from Jackson Laboratory and were maintained under the specific pathogen free condition. B6 mice were used for timed mating to produce E9.5 and E10.5 embryos. The embryos were harvested and their somite pair numbers were counted to confirm the proper developmental stage as previously described (Lux et al., 2008 (link); Yoshimoto et al., 2011 (link)). The YS and P-Sp tissues were digested with 0.125% collagenase (StemCell Technologies) for 5 min. After E10, P-Sp region is called AGM region. AGM region were digested with 0.25% collagenase for 30 min at 37°C. Sublethally irradiated (150 rad) NSG neonates (day 2–3) were used for E10.5 pre-HSC transplantation. Lethally irradiated (900 rad) congenic BoyJ mice were used as recipients for transplantation of YS- and P-Sp derived NICD-induced T progenitors. Sublethally irradiated adult NSG mice were also used as recipients. The experimental procedures were approved by the Institutional Animal Care and Use Committee (IACUC) at Indiana University and the Animal Welfare Committee (AWC) at UTHealth.
Ding J., Cardoso A.A., Yoshimoto M, & Kobayashi M. (2021). The Earliest T-Precursors in the Mouse Embryo Are Susceptible to Leukemic Transformation. Frontiers in Cell and Developmental Biology, 9, 634151.
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5

Mouse Model Handling and Euthanasia

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C57BL/6J, BALB/cJ, Ido1−/−, Rag1−/− and NOD-Scid IL2rγc−/− (NSG) mice were purchased from Jackson Laboratories (Bar Harbor, Maine, USA). All mice were maintained in pathogen-free, ventilated cages with irradiated food and autoclaved water at The University of Texas at Austin (UT Austin) or WuXi AppTec. Experimental procedures were approved by the Institutional Animal Care and Use Committee (IACUC) at UT Austin or WuXi AppTec. Mice were monitored daily and euthanized by CO2 asphyxiation and cervical dislocation prior to any signs of distress.
Triplett T.A., Garrison K.C., Marshall N., Donkor M., Blazeck J., Lamb C., Qerqez A., Dekker J.D., Tano Y., W.e.i.-.C.h.e.n.g.-.L.u., Karamitros C.S., Ford K., Tan B., Zhang M., McGovern K., Coma S., Kumada Y., Yamany M.S., Sentandreu E., Fromm G., Tiziani S., Schreiber T.H., Manfredi M., Ehrlich L.I., Stone E, & Georgiou G. (2018). Reversal of IDO-mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme. Nature biotechnology, 36(8), 758-764.
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6

Mouse Model Handling and Euthanasia

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C57BL/6J, BALB/cJ, Ido1−/−, Rag1−/− and NOD-Scid IL2rγc−/− (NSG) mice were purchased from Jackson Laboratories (Bar Harbor, Maine, USA). All mice were maintained in pathogen-free, ventilated cages with irradiated food and autoclaved water at The University of Texas at Austin (UT Austin) or WuXi AppTec. Experimental procedures were approved by the Institutional Animal Care and Use Committee (IACUC) at UT Austin or WuXi AppTec. Mice were monitored daily and euthanized by CO2 asphyxiation and cervical dislocation prior to any signs of distress.
Triplett T.A., Garrison K.C., Marshall N., Donkor M., Blazeck J., Lamb C., Qerqez A., Dekker J.D., Tano Y., W.e.i.-.C.h.e.n.g.-.L.u., Karamitros C.S., Ford K., Tan B., Zhang M., McGovern K., Coma S., Kumada Y., Yamany M.S., Sentandreu E., Fromm G., Tiziani S., Schreiber T.H., Manfredi M., Ehrlich L.I., Stone E, & Georgiou G. (2018). Reversal of IDO-mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme. Nature biotechnology, 36(8), 758-764.
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