Treated cells were collected by centrifugation, and the supernatant was discarded. The cells were washed twice with PBS and resuspended in 500 μl Binding Buffer (Wanleibio Co., Ltd., Shenyang, China). Next, 5 μL Annexin V-FITC (Wanleibio Co., Ltd.) and 10 μL propidium iodide (Wanleibio Co., Ltd.) were added and samples were mixed. Samples were incubated at room temperature away from light for 5-15 min. Analysis by flow cytometry was then performed.
Annexin 5 fitc
Manufactured by Wanlei
Sourced in China
Annexin V-FITC is a fluorescently labeled protein that binds to phosphatidylserine, a molecule that is exposed on the surface of apoptotic cells. It is commonly used in flow cytometry and other analytical techniques to detect and quantify apoptotic cells.
Automatically generated - may contain errors
4 protocols using annexin 5 fitc
1
Annexin V-FITC and Propidium Iodide Apoptosis Assay
2
Apoptosis Quantification by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 24 h of treatment, the cells were collected and washed twice with PBS. The supernatant was removed and cells were re-suspended by the binding buffer. Five microliters of Annexin V-FITC (Wanleibio, Shenyang, China) and 10 μL of PI were added, and cells were incubated at room temperature for half an hour in the dark. The fluorescence was determined by flow cytometric analysis (Beckman-Coulter, Miami, FL, USA).
3
Apoptosis Detection by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cells were collected and ltered through a 40-μm nylon cell strainer. The cells were washed with PBS and resuspended in binding buffer with Annexin V-FITC (Wanleibio, China) and Propidium Iodide (Wanleibio) at 4℃ for 15min and acquired on a FACS on CytoFLEX S (BECKMAN COULTER, USA). Data were analyzed using software (CytExpert 2.3).
4
Apoptosis Quantification in Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In brief, the cells were inoculated in 6-well plates at a density of 2 × 103 per well. ZDQ-0620, GSK2126458 or DMSO were treated for 48 h, and the collected cells were stained in the dark with Annexin V-FITC and PI (Wanlei, China) for 10-20 min. Finally, the cells were sorted and quantitatively analyzed by flow cytometry (Becton-Dickinson, NJ, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!