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Uridine diphosphate glucuronic acid udpga

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Uridine diphosphate glucuronic acid (UDPGA) is a chemical compound that serves as a cofactor in various enzymatic reactions. It is involved in the glucuronidation process, which is a crucial phase II metabolism pathway. UDPGA provides the glucuronic acid moiety for the conjugation of various endogenous and exogenous compounds, facilitating their elimination from the body.

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Lab products found in correlation

Alamethicin, by Merck Group (3 mentions) D saccharic 1, by Merck Group (2 mentions) E coli β glucuronidase, by Merck Group (2 mentions) 1 chloro 2 4dinitro benzene cdnb glycogen, by Merck Group (2 mentions) Glutathione (gsh), by Merck Group (2 mentions) Guanine, by Merck Group (2 mentions) O6 meg, by Merck Group (2 mentions) Anti gapdh antibody g8795, by Merck Group (1 mentions) Milli q integral water purification system, by Merck Group (1 mentions) Formic acid, by Tedia (1 mentions) Brij 58, by Merck Group (1 mentions) Lc grade methanol, by Tedia (1 mentions) Acetonitrile, by Tedia (1 mentions) Chrysin, by Meilun (1 mentions) Sn 38g, by LGC (1 mentions) Zidovudine o glucuronide, by LGC (1 mentions) Mgcl2, by Merck Group (1 mentions) β glucuronidase, by Merck Group (1 mentions) 4 lactone monohydrate, by Merck Group (1 mentions) Sucrose, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Uridine (351 citations) Glucuronic acid (86 citations) UDPGA (28 citations) Uridine diphosphate glucuronic acid (13 citations)

7 protocols using uridine diphosphate glucuronic acid udpga

1

Glucuronidation of Fluorogenic Probe

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The fluorogenic probe substrate N-butyl-4-(4-hydroxyphenyl)-1,8-naphthalimide (NHPN) and its O-glucuronide (NHPNG) were synthesized and purified (purity≥98%) according to the previously reported scheme [13 (link)]. Nilotinib and chrysin were from Dalian Meilun Biotech Co., Ltd. (Meilun, Dalian, China). The tissue preparations, including pooled human liver microsomes (HLM), pooled human intestine microsomes (HIM), pooled human kidney microsomes (HKM), and pooled human lung microsomes (HLuM) were purchased from Celsis Inc. (Baltimore, MD, USA). Recombinant human UGT1A1 was from BD Biosciences (Woburn, MA, USA). Stably transfected HeLa cells (named HeLa-UGT1A1 cells) were constructed as previously reported [[15] (link), [16] (link), [17] ]. The uridine diphosphate-glucuronic acid (UDPGA), polyethylene glycol hexadecyl ether (Brij 58) and anti-GAPDH antibody (G8795) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-UGT1A1 antibody (ab194697) was obtained from Abcam (Cambridge, MA). The reagents for the SimpleWestern blotting system were purchased from ProteinSimple (San Jose, CA). Ultrapure water purified by Milli-Q® Integral Water Purification System (Millipore, USA) was used throughout, while LC grade methanol, acetonitrile, and formic acid were ordered from Tedia (Fairfield, USA).
Zhu Y.D., Pang H.L., Zhou Q.H., Qin Z.F., Jin Q., Finel M., Wang Y.N., Qin W.W., Lu Y., Wang D.D, & Ge G.B. (2020). An ultra-sensitive and easy-to-use assay for sensing human UGT1A1 activities in biological systems. Journal of Pharmaceutical Analysis, 10(3), 263-270.
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2

In Vitro Glucuronidation Assay

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SN-38, genistein, propofol, tamoxifen and zidovudine were purchased from Yingxuan Chempharm Co., Ltd. (Shanghai, China), Mansite Pharmaceutical Co., Ltd. (Chengdu, China), Tokyo Chemical industry Co., Ltd. (Tokyo, Japan), Maya Reagent Co., Ltd (Zhejiang, China), and Melone Pharmaceutical Co., Ltd (Dalian, China), respectively. SN-38G, propofol glucuronide, tamoxifen N-β-D-glucuronide and zidovudine O-glucuronide were purchased from Toronto Research Chemicals (North York, Canada). Uridine diphosphate glucuronic acid (UDPGA), alamethicin, D-saccharic-1, 4-lactone monohydrate, MgCl2, and β-glucuronidase were purchased from Sigma-Aldrich (St. Louis, MO, USA). The primary antibodies of anti-UGT1A (sc-271268), anti-UGT1A4 (sc-27427), anti-UGT2B7 (ab126269), anti-UGT1A1 (ab458411), and anti-UGT1A9 (H00054600-A01) were obtained from Santa Cruz Biotechnology, Inc. (CA, USA), Abcam (Cambridge, MA), and Abnova (Taipei, Taiwan), respectively. All other chemicals and reagents used were of the highest commercially available grade.
Lu L., Zhou J., Shi J., Peng X.J., Qi X.X., Wang Y., Li F.Y., Zhou F.Y., Liu L, & Liu Z.Q. (2015). Drug-Metabolizing Activity, Protein and Gene Expression of UDP-Glucuronosyltransferases Are Significantly Altered in Hepatocellular Carcinoma Patients. PLoS ONE, 10(5), e0127524.
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3

Carcinogen-Induced Enzymatic Assay

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The carcinogen 1, 2-dimethylhydrazine (DMH) was obtained from Tokyo Chemical Industry. P-nitrophenyl-β-d-glucuronide, E coli β-glucuronidase, glutathione, uridine diphosphate glucuronic acid (UDPGA), 1-chloro-2,4dinitro benzene (CDNB), glycogen, guanine and O6-MeG were purchased from Sigma-Aldrich Chemical Co. Tris–HCl and sucrose were obtained from Thermo Fisher Scientific Inc. Phenol–chloroform–isoamylalcohol was obtained from Research Organics Inc. Other reagent grade chemicals were obtained from Merck Millipore Bioscience (Thailand).
Hu R., Chantana W., Pitchakarn P., Subhawa S., Chantarasuwan B., Temviriyanukul P, & Chewonarin T. (2022). Ficus dubia latex extract prevent DMH-induced rat early colorectal carcinogenesis through the regulation of xenobiotic metabolism, inflammation, cell proliferation and apoptosis. Scientific Reports, 12, 15472.
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4

Glucuronidation Profiling of Flavonoids

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Alamethicin, β-estradiol, dipyridamole (DIPY), D-saccharic-1, 4-lactone, magnesium chloride (MgCl2), MK-571, Ko143, leukotriene C4 (LTC4) and uridine diphosphate glucuronic acid (UDPGA) were all obtained from Sigma-Aldrich (St Louis, MO). Apigenin, chrysin, cycloicaritin, genistein, and wushanicaritin with purity over 98% was purchased from Aladdin Reagents (Shanghai, China). Apigenin-7-O-glucuronide, chrysin-7-O-glucuronide, genistein-7-O-glucuronide, and β-estradiol-3-O-glucuronide was purchased from Toronto Research Chemicals (North York, ON, Canada). Wushanicaritin-3-O-glucuronide was prepared in our laboratory as described previously [23 (link)]. 293T cells, HeLa cells, pLVX-mCMV-ZsGreen-PGK-Puro vector [9371 base pairs (bp)] and pLVX-shRNA2-Neo vector (9070 bp) were all provided from BioWit Technologies (Shenzhen, China). The pGEM-T plasmid which carried the UGT1A1 cDNA clone was purchased from Sino Biological Inc. (Beijing, China), while recombinant human UGT1A1 was purchased from Corning Biosciences (New York, USA). The anti-BCRP, anti-GAPDH, anti-MRP1, anti-MRP2, anti-MRP3, anti-MRP4 and anti-UGT1A1 antibodies were all obtained from OriGene Technologies (Rockville, MD). All other chemicals and reagents were the highest grade commercially available.
Li S., Xu J., Yao Z., Hu L., Qin Z., Gao H., Krausz K.W., Gonzalez F.J, & Yao X. (2018). The roles of breast cancer resistance protein (BCRP/ABCG2) and multidrug resistance-associated proteins (MRPs/ABCCs) in the excretion of cycloicaritin-3-O-glucoronide in UGT1A1-overexpressing HeLa cells. Chemico-biological interactions, 296, 45-56.
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5

Rat Liver Microsome Preparation

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Novartis proprietary compounds were obtained from the Novartis compound store as 10 mM stock solutions in Dimethyl sulfoxide (DMSO). β-Nicotinamide adenine dinucleotide 2′-phosphate tetrasodium salt (NADPH), uridine diphosphate glucuronic acid (UDPGA) and all other reagents, chemicals and buffer salts were purchased from Sigma-Aldrich Chemicals (St. Louis, MO, USA), Fluka (Buchs, Switzerland) or Merck (Darmstadt, Germany). Pools of male Sprague-Dawley rat liver microsomes from ≥ 3 individual rats (catalog number: M00001, lot number: YLQ) were obtained from BioIVT (Brussels, Belgium).
Trunzer M., Teigão J., Huth F., Poller B., Desrayaud S., Rodríguez-Pérez R, & Faller B. (2024). Improving In Vitro-In Vivo Extrapolation of Clearance Using Rat Liver Microsomes for Highly Plasma Protein-Bound Molecules. Drug metabolism and disposition: the biological fate of chemicals, 52(5).
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6

Raloxifene Glucuronidation Assay

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The pGL3-Promoter and pRL-TK renilla plasmids were obtained from Promega (Madison, WI). All synthesized DNA oligos used for 3’ UTR amplification, site-directed mutagenesis (SDM), and polymerase chain reaction (PCR) analysis were from Integrated DNA Technologies, Inc (Coralville, IA). Lipofectamine 2000 transfection reagent was from Life Technologies (Carlsbad, CA). miRVana miRNA mimic miR-491–3p (#4464066), negative control #1 mimic (#4464058), miRVana miRNA inhibitor miR-491–3p (#4464084), and negative control #1 inhibitor (#4464076) were purchased from Ambion (Austin, TX). Uridine diphosphate glucuronic acid (UDPGA), raloxifene, alamethicin, and bovine serum albumin (BSA) were obtained from Sigma-Aldrich (St. Louis, MO). Ral-6-glucuronide (ral-6-gluc), ral-4’-glucuronde (ral-4’-gluc), raloxifene-d4, ral-6-gluc-d4, ral-4’gluc-d4, and epirubicin hydrochloride were purchased from Toronto Research Chemical (Toronto, ON, Canada). Rabbit anti-UGT2B7 and anti-calnexin antibodies were from BD Biosciences (San Jose, CA) and Cell Signaling Technologies (Danvers, MA), respectively. Goat anti-rabbit secondary antibody conjugated to hydrogen peroxidase was from Thermo Scientific (Waltham, MA). All other chemicals used were purchased from Fisher Scientific (Pittsburg, PA) unless otherwise specified.
Dluzen D.F., Sun D., Salzberg A.C., Jones N., Bushey R.T., Robertson G.P, & Lazarus P. (2015). Regulation of UGT1A1 expression and activity by miR-491-3p*. Drug metabolism reviews, 47(3), 320-334.
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7

Carcinogen DMH Protocol for Bioassays

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The carcinogen 1, 2-dimethylhydrazine (DMH) was obtained from Tokyo Chemical Industry. P-nitrophenylβ-D-glucuronide, E coli β-glucuronidase, glutathione, uridine diphosphate glucuronic acid (UDPGA), 1chloro-2,4dinitro benzene (CDNB), glycogen, guanine and O 6 -MeG were purchased from Sigma-Aldrich Chemical Co. Tris-HCl and sucrose were obtained from Thermo Fisher Scienti c Inc. Phenol-chloroformisoamylalcohol was obtained from Research Organics Inc. Other reagent grade chemicals were obtained from Merck Millipore Bioscience (Thailand).
Hu R., Chantana W., Pitchakarn P., Subhawa S., Chantarasuwan B., Temviriyanukul P., & Chewonarin T. (2022). Ficus Dubia Latex Extract Prevent DMH-Induced Rat Colorectal Carcinogenesis Through the Regulation of Xenobiotic Metabolism, Inflammation, Cell Proliferation and Apoptosis.
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