Tlr3 agonist poly 1 c

Manufactured by InvivoGen

Sourced in United States, Germany

The TLR3 agonist poly(I:C) is a synthetic double-stranded RNA (dsRNA) that mimics the structure of viral RNA. It is a potent activator of the Toll-like receptor 3 (TLR3) signaling pathway, which plays a crucial role in the innate immune response against viral infections.

Automatically generated - may contain errors

Lab products found in correlation

Imiquimod, by InvivoGen (2 mentions) Abca1, by Novus Biologicals (1 mentions) Anti mouse hrp, by Santa Cruz Biotechnology (1 mentions) Anti rabbit hrp, by Santa Cruz Biotechnology (1 mentions) Cd11b percp cy5, by BioLegend (1 mentions) Mhcii apc, by Thermo Fisher Scientific (1 mentions) Gapdh, by Abcam (1 mentions) H3k27ac, by Abcam (1 mentions) Poly 1 c lmw, by InvivoGen (1 mentions) Monoclonal mouse igg1 clone 11711, by R&D Systems (1 mentions) Recombinant human tnf α, by R&D Systems (1 mentions) Poly da dt, by InvivoGen (1 mentions) Il 22, by R&D Systems (1 mentions) Il 1β, by R&D Systems (1 mentions) Pam3csk4, by InvivoGen (1 mentions) Gm csf, by R&D Systems (1 mentions) Lipopolysaccharides (lps), by InvivoGen (1 mentions) Ifn γ, by R&D Systems (1 mentions) Cpg odn 1826, by InvivoGen (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions)

6 protocols using tlr3 agonist poly 1 c

Macrophage Immunological Profiling

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies and conjugates were used in this study: anti‐F4/80 clone Cl:A3‐1. LXRα/β antiserum,^[⁴³ (link)
^] LXRα (Abcam#PPZ0412), H3K27Ac (Abcam#ab4729), NOS‐2 (Santa Cruz#SC‐650 M‐19), ABCA1 (Novus Biologicals #NB400‐105), GAPDH (Abcam #ab9485‐100), Anti‐mouse‐HRP (Santa Cruz#SC‐2005), Anti‐rabbit‐HRP (Santa Cruz#SC‐2004), CD11b‐PerCP‐Cy5.5 (Biolegend #clone M1/70), F4/80‐PE or FITC (Ebioscience #clone BM8), Ly6G‐PE or FITC (BD Pharmingen #clone AL21), MHC‐II‐APC (eBioscience #M5/114.15.2). The following pharmacological reagents were obtained from the MRC PPU Reagents University of Dundee, UK: BI605906 (IKKβ inhibitor), 5Z‐7‐oxozeanol (TAK1 inhibitor), MRT67307 (TBK1 inhibitor) and the following products from Calbiochem: PD0325901 (MEK/ERK inhibitor), PD98059 (MEK1 and MEK2 inhibitor), PIK‐75‐hydrochloride (inhibitor of p110α subunit of PI3‐Kinase), SB590885 (Raf‐1 inhibitor); HX531 (RXR inhibitor). TLR agonists, Poly I:C (TLR3 agonist) and ultrapure LPS from E. coli 0111:B4 strain‐ (TLR4 ligand) were obtained from Invivogen and were used at 10 µg ml⁻¹ and 100 ng ml⁻¹ respectively.

Vladimir de la Rosa J., Tabraue C., Huang Z., Orizaola M.C., Martin‐Rodríguez P., Steffensen K.R., Zapata J.M., Boscá L., Tontonoz P., Alemany S., Treuter E, & Castrillo A. (2024). Reprogramming of the LXRα Transcriptome Sustains Macrophage Secondary Inflammatory Responses. Advanced Science, 11(20), 2307201.

+ Open protocol

+ Expand

Viral Infection and Immune Response Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

HSV-1 virus stock (VR-733) was purchased from ATCC (Manassas, VA). PRR agonists CpG (ODN2395), CL264 (cat#: tlrl-c264e), LPS (cat#: tlrl-b5LPS), Pam3CSK4 (cat#: tlrl-pms), PolydA:dT (cat#: tlrl-patc), PolyI:C-LMW (cat#:tlrl-picwlv), polyI:C-TLR3 agonist (cat#: tlrl-picw), and SS40 (cat#: tlrl-lrna40) were purchased from InvivoGen (San Diego, CA). Recombinant human TNFα (cat# 210-TA-020/CF), IL-1β (cat# 201-LB-005), IFNγ, IL-4, IL-22, GM-CSF, monoclonal mouse IgG1 Clone#11711 (Cat# MAB002), human IL-1β/IL-1F2 antibody (MAB601), human TNFαR1/TNFRSF1A antibody (MAB625), and anti-human IFNγR1 antibody (MAB6731) were bought from R&D systems, Inc. (Minneapolis, MN). Recombinant human IFNα (cat#:11101-1) and mouse anti-human IFNα (cat#: 21112-1) were purchased from PBL Biomedical Laboratories (Piscataway, NJ). Myc-DDK1-tagged RELA (RC220780), Myc-DDK1-tagged NFKB1 (RC208384), Myc-DDK1-tagged IRF7 (RC217934), Myc-DDK1-tagged ANKRD1 (RC205609) and Myc-DDK1-tagged STING were purchased from OriGene (Rockville, MD). Dr. Hong-Bing Shu (Wuhan University, China) kindly provided pCMV-flag-IRF3 and pCMV-flag-MyD88. PRK-neo-HA-ANKRD1 was generated in our lab by insertion of an encoding cDNA fragment in frame into PRK-neo-HA vector (a kind gift from Dr. Hong-Bing Shu).

Bin L., Li X., Richers B., Streib J.E., Hu J.W., Taylor P, & Leung D.Y. (2018). ANKRD1 regulates innate immune responses against herpes simplex virus 1: A potential role in eczema herpeticum. The Journal of allergy and clinical immunology, 141(6), 2085-2093.e1.

+ Open protocol

+ Expand

Peptides for Immunogenicity Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peptides vimentin aa28-49wt (RSYVTTSTRTYSLGSALRPSTS), aa28-49cit (cit-SYVTTST-cit-TYSLGSAL-cit-PSTS), aa415-433wt (LPNFSSLNLRETNLDSLPL), aa415-433cit (LPNFSSLNL-cit-ETNLDSLPL) and enolase aa241-260cit (VIGMDVAASEFF-cit-SGKYDLD) and aa241-260wt (VIGMDVAASEFFRSGKYDLD) were synthesised at >90% purity by GenScript (USA) and stored lyophilised at −80°C. Prior to use, peptides were reconstituted to the appropriate concentration in PBS (Sigma, UK). Peptides linked via the N terminus to TLR1/2 ligand AMPLIVANT were manufactured by ISA Pharmaceuticals, Netherlands.29 (link) Adjuvants tested included murine GM-CSF (Miltenyi Biotec, Germany), TLR9 agonist CpG ODN 1826 (InvivoGen, USA), TLR4 agonist MPLA (Sigma), TLR3 agonist poly(I:C) (InvivoGen), TLR7 agonist Imiquimod (InvivoGen, USA) and TLR1/2 ligand AMPLIVANT (ISA Pharmaceuticals, Netherlands).30 (link)

Brentville V.A., Metheringham R.L., Daniels I., Atabani S., Symonds P., Cook K.W., Vankemmelbeke M., Choudhury R., Vaghela P., Gijon M., Meiners G., Krebber W.J., Melief C.J, & Durrant L.G. (2020). Combination vaccine based on citrullinated vimentin and enolase peptides induces potent CD4-mediated anti-tumor responses. Journal for Immunotherapy of Cancer, 8(1), e000560.

+ Open protocol

+ Expand

Immunostaining and FACS Analysis Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antibodies used for immunostaining were mouse anti-E glycoprotein (4G2) kindly provided by P. Despres (PIMIT, Université de La Réunion-INSERM France) and anti-Mouse IgG (H + L) Alexa Fluor 555 Secondary Antibody (Life Technologies). The antibodies used for western blot detection were anti-ISG15 (Santa Cruz), anti-flag (clone M2; Sigma-Aldrich) and mouse anti-actin (clone AC74; Sigma-Aldrich). The antibodies used for FACS analysis were APC-conjugated anti-CD163 (clone REA812, MACS Miltenyi Biotec), PE-conjugated mouse anti-HLA-DR (clone LN3, Invitrogen); PE-Cy7-conjugated mouse anti-CD11b (clone ICRF44, BD Biosciences), Pacific Blue-conjugated mouse anti-CD14 (clone 63D3, Biolegend). Other reagents include TLR3 agonist poly(I:C) (Invivogen); Hoescht (Life Technologies) and cDNA synthesis and qPCR kits (Life Technologies). The reagents for the preparation of the libraries include: Vivaspin unit 100 MWA (Sartorius); Pico Total RNA kit (Agilent), T4 RNA ligase (New England Biolabs); Superscript III (Life Technology); Ultra DNA Library Prep kit for Illumina (New England Biolabs): AMPure XP beads (Agencourt); and High Sensitivity DNA kit (Agilent).

Grass V., Hardy E., Kobert K., Talemi S.R., Décembre E., Guy C., Markov P.V., Kohl A., Paris M., Böckmann A., Muñoz-González S., Sherry L., Höfer T., Boussau B, & Dreux M. (2022). Adaptation to host cell environment during experimental evolution of Zika virus. Communications Biology, 5, 1115.

+ Open protocol

+ Expand

Cell Culture and Reagent Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Huh7.5.1 cells (Zhong et al., 2005 (link)) (RRID:CVCL_E049), Vero cells (CLS Cat# 605372/p622_VERO, RRID:CVCL_0059), and BHK-21 cells (ATCC Cat# CCL-10, RRID:CVCL_1915) were maintained in DMEM (Dulbecco's modified Eagle medium; Life Technologies) supplemented with 10% FBS, 100 units (U)/ml penicillin, 100 mg/ml streptomycin, 2 mM L-glutamine (Life Technologies). Cells were maintained at 37°C in 5% CO₂. MEFs were maintained in supplemented RPMI growth media. The work involved cell line, with routinely verify status for contamination (e.g. Mycoplasma).
Cell culture reagents included the TLR7 agonist (IMQ; imiquimod) and TLR7/8 agonist (R848) (Invivogen, San Diego, CA); TLR7 antagonist, IRS661 (5’-TGCTTGCAAGCTTGCAAGCA-3’) synthesized on a phosphorothioate backbone (MWG Biotech, Ebersberg, Germany); TLR3 agonist (poly-I:C) (Invivogen); TLR9 agonist (CpG) (Invivogen); recombinant IFNβ (PBL Interferon Source, Piscataway, NJ). General reagents included: paraformaldehyde (PFA) and brefeldin A (BFA) (Sigma-Aldrich, St Louis, MO); Ficoll-Hypaque (GE Healthcare Life Sciences, Piscataway, NJ).

Webster B., Werneke S.W., Zafirova B., This S., Coléon S., Décembre E., Paidassi H., Bouvier I., Joubert P.E., Duffy D., Walzer T., Albert M.L, & Dreux M. (2018). Plasmacytoid dendritic cells control dengue and Chikungunya virus infections via IRF7-regulated interferon responses. eLife, 7, e34273.

+ Open protocol

+ Expand

HCV Genome Sensing and Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hepatoma Huh7.5 cell line (provided by Dr. Charles Rice, Rockefeller University) and Huh7.5–20 producing JFH1 HCV (provided by Dr. T. Jake Liang, National Institutes of Health) were cultured in DMEM-based complete growth medium. The 293HEK cell line expressing TLR3, TLR7 or TLR8, and the plasmid pNiFty2-Luc were obtained from Invivogen (USA). The pNiFty2-Luc plasmid contains a reporter gene expression cassette that allows firefly luciferase production under the control of NF-κB. Nine sequences within the HCV genome containing around 65% GU were selected and synthesized (Integrated DNA Technologies, Canada). TLR7/8 agonist RNA40, CL097 and TLR3 agonist poly I:C were obtained from Invivogen. DC-SIGN blocking antibody and matched isotype control were obtained from R&D Systems.

Zhang Y., El-Far M., Dupuy F.P., Abdel-Hakeem M.S., He Z., Procopio F.A., Shi Y., Haddad E.K., Ancuta P., Sekaly R.P, & Said E.A. (2016). HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses. Scientific Reports, 6, 29447.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!