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Fitc conjugated anti mouse cd4 clone gk1.5

Manufactured by Miltenyi Biotec
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Sourced in Germany

The FITC-conjugated anti-mouse CD4 (clone GK1.5) is a flow cytometry reagent designed to detect the CD4 surface marker on mouse cells. It is conjugated with the fluorescent dye FITC (Fluorescein Isothiocyanate) to enable fluorescent detection and analysis of CD4-positive cells.

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Lab products found in correlation

Goat anti rat alexa 568, by Thermo Fisher Scientific (2 mentions) Apc conjugated anti mouse f4 80 clone bm8, by Thermo Fisher Scientific (2 mentions) Antibody to mouse cd31 clone 2h8, by Bio-Rad (2 mentions) Antibody to neun, by Merck Group (2 mentions) Antibodies to neurofilament 200 and mbp, by Abcam (2 mentions) Monoclonal antibody to β gal clone gal 13, by Merck Group (2 mentions) Pe conjugated anti mouse cd45 clone 30 f11, by BD (2 mentions) Apc conjugated anti mouse cd11b clone m1 70, by BD (2 mentions) Pe conjugated anti mouse ly6g clone 1a8, by BD (2 mentions) Percp conjugated anti mouse cd8 clone 53 6.7, by Miltenyi Biotec (2 mentions) Fitc conjugated anti mouse il 17a clone ebio17b7, by Thermo Fisher Scientific (2 mentions) Apc conjugated anti mouse ifn γ clone xmg1.2, by Thermo Fisher Scientific (2 mentions) Fitc conjugated anti mouse cd45, by Thermo Fisher Scientific (2 mentions) Rat anti mouse cd8α clone 53 6.7, by Novus Biologicals (2 mentions) Goat anti rabbit alexa 488, by Thermo Fisher Scientific (2 mentions)

2 protocols using fitc conjugated anti mouse cd4 clone gk1.5

1

Immunohistochemical Staining of Mouse Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibody to mouse CD31 (clone 2H8) was purchased from AbDSerotec (Kidlington, UK) and rabbit polyclonal Antibody to NeuN was from Millipore (Darmstadt, Germany). Antibodies to neurofilament 200 and MBP were from Abcam (Cambridge, UK). Monoclonal antibody to β-gal (clone GAL-13) was from Sigma (St. Louis, MO). PE-conjugated anti-mouse CD45 (clone 30-F11), APC-conjugated anti-mouse CD11b (clone M1/70) and PE-conjugated anti-mouse Ly6G (clone 1A8) were from BD Biosciences (Heidelberg, Germany). FITC-conjugated anti-mouse CD4 (clone GK1.5) and PerCP-conjugated anti-mouse CD8 (clone 53-6.7) were from Miltenyi Biotec (Bergisch Gladbach, Germany). APC-conjugated anti-mouse F4/80 (clone BM8), FITC-conjugated anti-mouse IL-17A (clone eBio17B7) and APC-conjugated anti-mouse IFN-γ (clone XMG1.2) and FITC-conjugated anti mouse CD45 were from eBioscience (Frankfurt, Germany). Rat anti-mouse CD8α (clone 53-6.7) was from Novusbio (Littleton, CO). Rabbit polyclonal antibody to IL-17 was from Abcam (Cambridge, UK). Goat anti-rat Alexa 568 and goat anti-rabbit Alexa 488 were from Life Technologies (Darmstadt, Germany). Del-1-Fc was constructed, expressed, and purified as previously described17 (link).
Choi E.Y., Lim J.H., Neuwirth A., Economopoulou M., Chatzigeorgiou A., Chung K.J., Bittner S., Lee S.H., Langer H., Samus M., Kim H., Cho G.S., Ziemssen T., Bdeir K., Chavakis E., Koh J.Y., Boon L., Hosur K., Bornstein S.R., Meuth S.G., Hajishengallis G, & Chavakis T. (2014). Developmental endothelial locus-1 (Del-1) is a homeostatic factor in the central nervous system limiting neuroinflammation and demyelination. Molecular psychiatry, 20(7), 880-888.
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2

Immunohistochemical Staining of Mouse Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibody to mouse CD31 (clone 2H8) was purchased from AbDSerotec (Kidlington, UK) and rabbit polyclonal Antibody to NeuN was from Millipore (Darmstadt, Germany). Antibodies to neurofilament 200 and MBP were from Abcam (Cambridge, UK). Monoclonal antibody to β-gal (clone GAL-13) was from Sigma (St. Louis, MO). PE-conjugated anti-mouse CD45 (clone 30-F11), APC-conjugated anti-mouse CD11b (clone M1/70) and PE-conjugated anti-mouse Ly6G (clone 1A8) were from BD Biosciences (Heidelberg, Germany). FITC-conjugated anti-mouse CD4 (clone GK1.5) and PerCP-conjugated anti-mouse CD8 (clone 53-6.7) were from Miltenyi Biotec (Bergisch Gladbach, Germany). APC-conjugated anti-mouse F4/80 (clone BM8), FITC-conjugated anti-mouse IL-17A (clone eBio17B7) and APC-conjugated anti-mouse IFN-γ (clone XMG1.2) and FITC-conjugated anti mouse CD45 were from eBioscience (Frankfurt, Germany). Rat anti-mouse CD8α (clone 53-6.7) was from Novusbio (Littleton, CO). Rabbit polyclonal antibody to IL-17 was from Abcam (Cambridge, UK). Goat anti-rat Alexa 568 and goat anti-rabbit Alexa 488 were from Life Technologies (Darmstadt, Germany). Del-1-Fc was constructed, expressed, and purified as previously described17 (link).
Choi E.Y., Lim J.H., Neuwirth A., Economopoulou M., Chatzigeorgiou A., Chung K.J., Bittner S., Lee S.H., Langer H., Samus M., Kim H., Cho G.S., Ziemssen T., Bdeir K., Chavakis E., Koh J.Y., Boon L., Hosur K., Bornstein S.R., Meuth S.G., Hajishengallis G, & Chavakis T. (2014). Developmental endothelial locus-1 (Del-1) is a homeostatic factor in the central nervous system limiting neuroinflammation and demyelination. Molecular psychiatry, 20(7), 880-888.
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