Anti a3ar sc 13938

Differentiated cells (adherent cells) and GSCs of the U87MG cell line were evaluated by flow cytometry (BD C6 Accuri and BD FACSJazz™, USA). Briefly, 1.0 × 10⁶ cells were fixed with PFA 3.7% for 15 min at room temperature and permeabilized with cold methanol for 45 min at 4°C. Cells were then blocked for 45 min with 1 × PBS-BSA 0.5% at room temperature. Lastly, the cells were marked with the anti-MRP1 (sc-18835, Santa Cruz), anti-A₃AR (sc-13938, Santa Cruz), anti-Nestin (sc-23927, Santa Cruz), and anti-CD133 (130-092-395, Miltenyi Biotec) antibodies at 4°C overnight and were detected by flow cytometry using their respective Alexa fluor 488 antibody (Life Technology) through the FL1 filter (530/30 nm) of the cytometer.

Rat and human kidney tissues were fixed in formalin, embedded in paraffinand 5 μm sections were mounted on silanized slides. Immunodetections were performed as described previously [20 (link)] using the primary polyclonal anti-ENT1 antibody from Protein Tech (II 337-1-A7) and the monoclonal anti-αSMA (sc130617) and anti-A₃AR (sc-13938) from Santa Cruz Biotechnology. The immunosignals were detected using the LSAB+ System–HRP (DakoCytomation).