Cocl2 6h2o

The bacterial strain Pseudomonasputida MnB1 [American Type Culture Collection (ATCC) no. 23483] was cultured in the Lept medium (0.5 g/L yeast extract (Ruji, Shanghai, China), 0.5 g/L Casamino Acids (Coolaber, Beijing, China), 5 mM D(+)-glucose (Macklin, Shanghai, China), 10 mM HEPES (N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid, pH 7.5, GBCBIO, Guangzhou, China), 0.48 mM CaC1₂ (Macklin), 0.83 mM MgSO₄ (Macklin), 3.7 μM FeCl₃ (Macklin), and 1 mL of trace element solution (10 mg/L CuSO₄•5H₂O, 44 mg/L ZnSO₄•7H₂O, 20 mg CoCl₂•6H₂O and 13 mg/L Na₂MoO₄•2H₂O, (Macklin)) containing 1mM MnCl₂ (Aladdin, Shanghai, China) at 30 °C and shaken at 150 rpm for 5 d [48 (link)]. The suspensions were centrifuged at 8000× g for 20 m and the supernatant was discarded, sediments of bacteria and BMO were diluted three times with deionized water by means of centrifugation (20 m at 8000× g), then the mixture of precipitates were collected for freeze-drying to obtain the dried BMO sample.

All bacterial strains and plasmids used in this study are listed in Table S1. Throughout the study, the P. putida strains were grown in Lysogeny broth (LB) medium at 28 °C, and the E. coli strains were grown in LB medium at 37 °C. When required, antibiotics (purchased from Sangon Biotech, Shanghai, China) were used at the following concentrations: chloramphenicol (25 mg/L), gentamycin (20 mg/L), tetracycline (20 mg/L), kanamycin (50 mg/L) and ampicillin (150 mg/L). Analytical-grade salts of CdCl₂·2.5H₂O, CoCl₂·6H₂O, CrCl₃·6H₂O, CuCl₂·2H₂O, MnCl₂·4H₂O, NiCl₂·6H₂O, Pb(NO₃)₂, and ZnCl₂ (purchased from Macklin, Shanghai, China) were used to prepare 1 M stock solutions, which were sterilized by filtration.

CoCl₂·6H₂O, tannin, silver acetate, 3,3',5,5'-tetramethylbenzidine (TMB), o-phenylenediamine (OPD) and gelatin were obtained from Macklin Biochemical Co., Ltd. (Shanghai, China). Acrylic anhydride was purchased from Sigma (St Louis, MO, USA). Hydrogen peroxide (H₂O_2, 30%), aqueous ammonia, acetic acid and ethanol were the products of Tianjin Kermel Chemical Plant (Tianjin, China). 2,2-Dimethoxy-2-phenylacetophenone (DMPA), the live/dead (SYTO 9/PI) bacterial viability kit and PE anti-mouse CD45 (Cat# 12-0451-82) were received from Thermo Fisher Scientific (New Jersey, USA). The lactate dehydrogenase (LDH) cytotoxicity assay kit was obtained from Beyotime (Shanghai, China). 2',7'-Dichlorodihydrofluorescein diacetate (DCFH-DA) was obtained from Solarbio Life Science (Beijing, China). FITC anti-mouse F4/80 (Cat# 123108) and FITC anti-mouse Ly-6C/Ly-6C (Gr-1) (Cat# 108406) were obtained from Biolegend (San Diego, CA, USA). Anti-Ly6G + Ly6c (ab25377) and anti F4/80 (ab111101) were purchased from Abcam (Cambridge, UK). GTVisionTM I detection system/Mo&Rb (GK500510A) was received from Gene Tech (Shanghai) company limited. Four strains of MDR P. aeruginosa (21027, 20059, 21173, 21715) were kindly provided by Qingdao Municipal Hospital (China). Doubly deionized water was purified by a Unique-R20 purification system (Xiamen, China).