Bio reagent grade transferrin

The 25 mg/mL stock solutions of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dipalmitoyl-sn-glycerol-3-phosphot-rac(1-glycerol) ammonium salt (DPPG) were obtained from Avanti Polar Lipids, Inc. (Alabama, USA). Reagent-grade purified human immunoglobulin (IgG), lyophilized human albumin, Bio-reagent-grade transferrin, Sigma-grade cholesterol, ascorbate, urate, certified reference material-grade glutathione and Hank’s Balanced Salt Solution (HBSS) were supplied by Sigma-Aldrich Company Limited (Dorset, UK). HPLC-grade chloroform and water were supplied by Fischer Chemicals (Loughborough, UK). 6α,9-Difluoro-17-[[(fluoromethyl)sulfanyl]carbonyl]-11β-hydroxy-16α-methyl-3-oxo androsta-1,4-dien-17α-yl propanoate (fluticasone propionate: FP) was purchased from Adooq Bioscience (Irwin, CA), 9-Chloro-11β-hydroxy-16β-methyl-3,20-dioxopregna-1,4-diene-17,21-diyl dipropanoate (beclomethasone dipropionate; BDP) was purchased from Medchem Express (US), and Survanta® from AbbVie Ltd. (UK).

The 25 mg/mL stock solutions of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phospho-(1′-rac-glycerol) sodium salt (DPPG), both >99% purity, were obtained from Avanti Polar Lipids, Inc. (Alabama, USA). Reagent-grade purified human immunoglobulin (IgG), lyophilised human serum albumin, Bioreagent-grade transferrin, cholesterol, ascorbate, urate, certified reference material-grade glutathione and BioReagent grade gentamicin solution were supplied by Sigma-Aldrich Company Limited (Dorset, UK). Hanks' Balanced Salt Solution (HBSS), phenol red-free, was also supplied by Sigma-Aldrich and consisted of: 0.19 g/L calcium chloride dihydrate, 0.09 g/L magnesium sulphate anhydrous, 0.40 g/L potassium chloride, 0.06 g/L potassium phosphate, 0.35 g/L sodium bicarbonate, 8.00 g/L sodium chloride, 0.05 g/L sodium phosphate dibasic, and 1.00 g/L d-Glucose. HPLC-grade chloroform and methanol was supplied by Fischer Chemicals (Loughborough, UK). 25% ammonium hydroxide, sodium chloride and 2 M hydrochloric acid solutions were obtained from Sigma-Aldrich Company Limited (Dorset, UK).