Skin samples were lysed and the protein was resolved by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The membrane fractions were incubated with MyD88 (4283; Cat. no. 4283; Cell Signaling Technology, Inc., Danvers, MA, USA), rabbit-TLR8 (Cat. no. ab180610; Abcam) and mouse anti-β-tubulin (Cat. no. YM3030; Immunoway) antibodies, followed by IRDye 700DX- or 800DX-conjugated secondary antibodies (Rockland Immunochemicals Inc., Gilbertsville, PA, USA). Immunofluorescence was assessed by Odyssey infrared imaging system (LI-COR Biosciences, Lincoln, NE, USA).
Rabbit tlr8
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit-TLR8 is a recombinant protein that represents the extracellular domain of the human Toll-like receptor 8 (TLR8) protein. TLR8 is a member of the Toll-like receptor family and plays a role in the innate immune response.
Automatically generated - may contain errors
Lab products found in correlation
Odyssey infrared imaging system, by LI COR (1 mentions)
Myd88, by Cell Signaling Technology (1 mentions)
Mouse anti β tubulin, by Immunoway (1 mentions)
Bh 2 microscope, by Olympus (1 mentions)
Cd14 pe, by BD (1 mentions)
Vectashield with dapi, by Vector Laboratories (1 mentions)
Zenon kit, by Thermo Fisher Scientific (1 mentions)
Fluoview fv10i confocal microscope system, by Olympus (1 mentions)
2 protocols using rabbit tlr8
1
Immune Receptor Protein Expression Analysis
2
Immunofluorescence and Immunohistochemical Staining of Monocytes and Skin Tissues
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Monocytes were stained with mouse monoclonal antibodies against CD14-PE (BD Pharmingen), BFRF1 [18 (link)], gp-350-220 [13 (link)], rabbit TLR8 (Cell Signaling), and secondary-antibodies-Cy3-conjugated (Jackson IR, West Grove, PA, USA), Alexafluor-350 goat-anti mouse antibody (Invitrogen, Grand Island, NY, USA), or 488-labeling (Zenon kit; Invitrogen). Coverslips were mounted using Vectashield with DAPI (Vector Laboratories, Burlingame, CA, USA) and immunofluorescence staining was examined using a FluoView FV10i confocal microscope system (Olympus, Center Valley, PA, USA) at 488 nm (green), 594 nm (red), and 405 nm (blue). Paraffin sections of skin tissues were stained using mouse mAb against CD163 (AbD Serotec, Raleigh, NC, USA) and Zta/Zebra (Argene, bioMérieux, Inc., Durham, NC, USA) as previously described [13 (link)]. Immunohistochemical staining was examined using the Olympus BH2 microscope.
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