Pan t cell isolation beads

Manufactured by Miltenyi Biotec

Sourced in Canada

Pan T cell isolation beads are magnetic particles coated with antibodies that bind to the surface of T cells. These beads can be used to efficiently isolate and enrich T cells from a variety of sample types, including peripheral blood, tissue, and cell cultures.

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Lab products found in correlation

Histopaque, by Miltenyi Biotec (1 mentions) Interleukin 2 (il 2), by Thermo Fisher Scientific (1 mentions) Interleukin 2 (il 2), by Miltenyi Biotec (1 mentions)

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Pan-T-cell beads (3 citations)

2 protocols using pan t cell isolation beads

Isolation of Purified T Cells

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Peripheral blood mononuclear cells were isolated from fresh blood from healthy donors on a Histopaque gradient, mixed with pan T cell isolation beads (Miltenyi Biotec Inc., Auburn, CA). CD3 positive cells were negatively selected on a MACS column. The purified cells were kept in RPMI in humidified incubator and used within two hours.

Dinic J., Riehl A., Adler J, & Parmryd I. (2015). The T cell receptor resides in ordered plasma membrane nanodomains that aggregate upon patching of the receptor. Scientific Reports, 5, 10082.

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Isolation and Maintenance of iNKT and Polyclonal T Cells

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iNKT cells were initially produced by sorting single cells from blood of healthy subjects using α-GalCer loaded CD1d-Fc tetramers, as described (22 (link)). iNKT cells were maintained in IL-2 containing medium (RPMI 1640, 15% heat-inactivated bovine calf serum from Hyclone, 3% human AB serum from Gemini, 2 mM L-glutamine, 100 μg/ml penicillin and streptomycin), and were periodically stimulated to proliferate by the addition of PHA and irradiated PBMCs. Polyclonal T cells were isolated from PBMCs of healthy subjects by magnetic sorting using negative selection (pan T cell isolation beads, Miltenyi Biotech), then expanded in culture using PHA and irradiated PBMC in IL-2 containing medium. Prior to use in experiments, iNKT cells and polyclonal T cells were cultured for at least two weeks without addition of PHA or irradiated PBMCs, but in medium supplemented with 200 IU/mL IL-2 (Peprotech).

Felley L.E., Sharma A., Theisen E., Romero-Masters J.C., Sauer J.D, & Gumperz J.E. (2016). Human iNKT cells induce IL-1β secretion by peripheral blood monocytes via a P2X7-independent pathway. Journal of immunology (Baltimore, Md. : 1950), 197(6), 2455-2464.

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