Agilent 1100 hplc msd ion trap xct system

High-performance liquid chromatography coupled with tandem mass spectrometry (HPLC–MS/MS) was performed using an Agilent 1100 HPLC-MSD Ion Trap XCT system, equipped with an electrospray ion source (HPLC-ESI-MS) (Agilent Technologies, Santa Clara, CA, USA). Separation of extracts was performed on a Jupiter C18 column 1 × 150 mm with 3.5 μm particle size (Phenomenex, Torrance, CA, USA). As eluents, we used water (eluent A) and MeOH (eluent B), both added with 0.1% formic acid. The gradient employed was 15% eluent B for 5 min, linear to 100% eluent B in 35 min, and finally hold at 100% eluent B for another 5 min. The flow rate was set to 50 μL/min with a column temperature of 30 °C. The injection volume was 8 μL. Ions were detected in the positive and negative ion mode, in the m/z 100–800 range, and ion charged control with a target ion value of 100,000 and an accumulation time of 300 ms. A capillary voltage of 3300 V, nebulizer pressure of 20 psi, drying gas of 8 L/min, dry temperature of 325 °C, and 2 rolling averages (averages: 5) were the parameters set for the MS detection. MS/MS analysis was conducted using an amplitude optimized time by time for each compound. From the chromatograms, the percentage of PC for each extract was calculated on the basis of the peak area.

High-performance liquid chromatography coupled with mass spectrometry (HPLC–MS and MS/MS) was performed by using an Agilent 1100 HPLC-MSD Ion Trap XCT system, equipped with an electrospray ion source (HPLC-ESI-MS) (Agilent Technologies, Santa Clara, CA, USA). Separation of extracts was performed on a Zorbax SB C-18 column 1 mm × 150 mm with 3.5 μm particle size (Agilent Technologies). As eluents, water (eluent A) and acetonitrile (eluent B), both with added 0.1% formic acid, were used. The gradient employed was initial, 2% B; 0–5 min, linear up to 10% B; 15–15 min, isocratic 10% B, 10–15 min, linear up to 20% B; 20–25 min, liner up to 30% B; 25–40 min, linear up to 60% B; 40–45 min, linear up to 100% B; and 45–50 min, isocratic 100%. The column was then reconditioned at initial condition in 15 min. The flow rate was set to 30 µL/min with a column temperature of 30 °C. The injection volume was 8 μL. Ions were detected in the negative ion mode, in the 200–1600 m/z range. Ion charged control with a target ion value of 50,000 and an accumulation time of 300 ms were set. A capillary voltage of 3500 V, nebulizer pressure of 10 psi, drying gas of 8 L/min, dry temperature of 325 °C, and 3 rolling averages (averages: 5) were the parameters set for the MS detection. MS/MS analysis was conducted by using an amplitude optimized time by time for each compound.