Spleen tissues were fixed in 10% buffered formalin solution for 2 days and processed for paraffin sections using standard histology procedures. Paraffin sections were dewaxed in 100% xylenes and rehydrated in series concentrations of alcohols of 100, 95, 70% to water. Then, antigen retrieval procedure was performed using antigen unmasking solution (Vector Labs, Burlingame, CA). Slides were then incubated with rabbit anti-rat PD-1 (1:50 dilution, Abcam, Cambridge, MA) and rabbit anti-rat HLA-DR (1:50 dilution, Proteintech, Chicago, IL) antibodies overnight at 4 °C. Slides were washed with Tris-buffered saline containing 0.02% Triton x-100 and further reacted with biotinylated anti-rabbit IgG (Vector Labs). Then, the slides were incubated with peroxidase conjugated avidin followed by reaction with substrate, DAB (Vector Labs). Slides were counterstained with hematoxylin and evaluated using a Nikon microscope.
Rabbit anti rat hla dr
Manufactured by Proteintech
Rabbit anti-rat HLA-DR is a primary antibody that binds specifically to the HLA-DR antigen expressed on the surface of rat cells. It can be used for the detection and analysis of HLA-DR expressing cells in research applications.
Automatically generated - may contain errors
Lab products found in correlation
3 3 diaminobenzidine (dab), by Vector Laboratories (3 mentions)
Biotinylated anti rabbit igg, by Vector Laboratories (3 mentions)
Antigen unmasking solution, by Vector Laboratories (3 mentions)
Rabbit anti rat pd 1, by Abcam (3 mentions)
Odyssey system, by LI COR (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
4 protocols using rabbit anti rat hla dr
1
Immunohistochemical Analysis of Spleen PD-1 and HLA-DR
2
Spleen Tissue Protein Expression Analysis
3
Immunohistochemical Analysis of Spleen Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Spleen tissues were xed in 10% buffered formalin solution for 2 days and processed for para n sections using standard histology procedures. Para n sections were dewaxed in 100% xylenes and rehydrated in series concentrations of alcohols of 100%, 95%, 70% to water. Then, antigen retrieval procedure was performed using antigen unmasking solution (Vector Labs, Burlingame, CA). Slides were then incubated with rabbit anti-rat PD-1 (1:50, Abcam, Cambridge, MA) and rabbit anti-rat HLA-DR (1:50 dilution, Proteintech, Chicago, IL) antibodies overnight at 4 o C. Slides were washed with Tris-buffered saline containing 0.02% triton x-100 and further reacted with biotinylated anti-rabbit IgG (Vector Labs).
Then, the slides were incubated with peroxidase conjugated avidin followed by reaction with substrate, DAB (Vector Labs). Slides were counterstained with hematoxylin and evaluated using a Nikon microscope.
Then, the slides were incubated with peroxidase conjugated avidin followed by reaction with substrate, DAB (Vector Labs). Slides were counterstained with hematoxylin and evaluated using a Nikon microscope.
4
Immunohistochemical Analysis of PD-1 and HLA-DR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Spleen tissues were fixed in 10% buffered formalin solution for 2 days and processed for paraffin sections using standard histology procedures. Paraffin sections were dewaxed and rehydrated in xylenes and series concentrations of alcohols, followed by antigen retrieval using antigen unmasking solution (Vector Labs, Burlingame, CA). Slides were then incubated with rabbit anti-rat PD-1 (1:50, Abcam, Cambridge, MA) and rabbit anti-rat HLA-DR (1:50 dilution, Proteintech, Chicago, IL) antibodies overnight at 4 o C. Slides were washed with Tris-buffered saline containing 0.02% triton x-100 and further reacted with biotinylated anti-rabbit IgG (Vector Labs). Then, the slides were incubated with peroxidase conjugated avidin followed by reaction with substrate, DAB (Vector Labs). Slides were counterstained with hematoxylin and evaluated using a Nikon microscope.
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