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Cd39 pecy7

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CD39-PECy7 is a fluorochrome-conjugated antibody that recognizes the CD39 cell surface antigen. CD39 is an ectonucleotidase that catalyzes the hydrolysis of ATP and ADP to AMP. This product can be used for the identification and analysis of CD39-expressing cells by flow cytometry.

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Lab products found in correlation

Cd103 apc, by Thermo Fisher Scientific (3 mentions) Cd45.1 pe, by Thermo Fisher Scientific (2 mentions) F4 80 percp cy5, by BioLegend (2 mentions) Cd11b bv650, by BioLegend (2 mentions) Pd l1 pe, by BioLegend (2 mentions) Cd4 bv421, by BioLegend (2 mentions) Pd1 bv605, by BD (2 mentions) Cd90.1 fitc, by Thermo Fisher Scientific (2 mentions) Cd45.2 alexa700, by Thermo Fisher Scientific (2 mentions) Cd8α percp cy5, by Thermo Fisher Scientific (2 mentions) Cd24 apc cy7, by Thermo Fisher Scientific (2 mentions) Cd44 apcy7, by Thermo Fisher Scientific (2 mentions) Cd90.2 alexa 700, by BioLegend (2 mentions) Mhcii bv421, by BioLegend (2 mentions) Cd8α pe texas red, by Thermo Fisher Scientific (2 mentions) Ly6c bv711, by BioLegend (2 mentions) Cd3e pe, by BD (2 mentions) Cd45 bv786, by BD (2 mentions) Cd62l pe cy7, by Thermo Fisher Scientific (2 mentions) Cd4 fitc, by Thermo Fisher Scientific (2 mentions)

4 protocols using cd39 pecy7

1

Multiparametric Flow Cytometry of Tumor Cells

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Single-cell suspensions of tumors were prepared, and flow cytometry was carried out as mentioned earlier [45 (link), 46 (link)]. Cells were stained with the following antibodies obtained from BioLegend: CD16/32 (clone 93, catalog No. 103132), Zombie UV™ Fixable Viability Kit (catalog No. 100752), CD45-PerCP/Cy5.5 (clone 30-F11, catalog No. 103132), CD45-APC/Cy7 (clone 30-F11, catalog No. 103116), CD11b-FITC (clone M1/70, catalog No. 101206), SIRPα-PE (clone BM8, catalog No. 123122), Gr-1-PerCP/Cy5.5 (clone RB6-8C5, catalog No. 108428), F4/80-AF647 (clone N418, catalog No. 117318),CD11c-PE/Cy7 (clone N418, catalog No. 117318), I-A/I-E-AF700 (clone M5/114.15.2, catalog No. 107622), CD86-BV421 (clone GL-1, catalog No. 105032), CD206-BV605 (clone C068C2, catalog No. 141721), CD8a-AF700 (clone 53-6.7, catalog No. 100730), CD8-BV510 (clone 53-6.7, catalog No. 100752), TNF-α-BV421 (clone MP6-XT22, catalog No. 506328), IFN-γ-PE (clone XMG1.2, catalog No. 505808), CD39-PE/Cy7 (clone Duha59, catalog No. 143806), Tim-3-APC/Fire™ 750 (clone RMT3-23, catalog No. 119738), PD-1-BV605 (clone 29 F.1A12, catalog No. 135220). The next antibodies were obtained through eBioscience: Mouse Regulatory T Cell Staining Kit #2 (clone FJK-16s, catalog No. 88-8118-40). FlowJo program (Tree Star Inc.) was utilized to examine the findings that were acquired through a BD LSRFortessa Flow Cytometer.
Ji K., Zhang Y., Jiang S., Sun L., Zhang B., Hu D., Wang J., Zhao L., Wang P, & Tao Z. (2023). SIRPα blockade improves the antitumor immunity of radiotherapy in colorectal cancer. Cell Death Discovery, 9, 180.
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2

Multiparametric Flow Cytometry Analysis

Cited 1 time
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Flow-cytometry antibodies included CD3e-PE, PD1-BV605, CD45-BV786 (BD-Biosciences), CD90.1-FITC, CD62L-PECy7, CD45.1-APC, CD45.1-PE (eBioscience), CD45.2-Alexa700, CD-8α-Percp-Cy5.5, CD4-FITC, CD103-APC, CD24-APCcy7 (Invitrogen), CD44-APCy7, CD4-BV421, F4/80-PercpCy5.5, CD39-PECy7, PDL1-PE, CD90.2-Alexa 700, MHCII-BV421, CD11b-BV650, Ly6C-BV711 (Biolegend), CD8α-PE-Texas-red (Life Technologies, Carlsbad, CA, USA). Blocking PD-1 antibody (clone RMP1-14, BioXCell, Branford, CT, USA) was administered systemically by intraperitoneal injections of 250 μg [41 (link)]. Blocking anti-CD40L (clone MR1, BioXCell) was administered systemically by intraperitoneal injections of 250 μg [19 (link)].
Sharon S., Baird J.R., Bambina S., Kramer G., Blair T.C., Jensen S.M., Leidner R.S., Bell R.B., Casap N., Crittenden M.R, & Gough M.J. (2021). A platform for locoregional T-cell immunotherapy to control HNSCC recurrence following tumor resection. Oncotarget, 12(13), 1201-1213.
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3

Comprehensive Flow Cytometry Analysis of Immune Cells

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For flow cytometry analysis of lymph nodes (LN), spleen (SP), tumors, and AT, the following antibodies and buffers were used: CD3-PE, CD4-BV510, CD8-PeCy7, F4/80-APC, MHCII-AF488, CD206-BV605 CCR8-BV421, CD39-PeCy7, NRP1-PE and PD1-BV711 from Biolegend; CD25-PeCy5.5 from Thermo Scientific and CD11b-VF450 from Tonbo. For intracellular analysis, we used anti-Foxp3-APC (Tonbo) and Helios-FITC (Biolegend), using the Foxp3/Transcription Factor Staining Buffer Kit (Tonbo) for fixation and permeabilization of the cells.
All cells were initially stained with viability dye Zombie-NIR (Biolegend) and samples were acquired in an Attune NxT® Acoustic Focusing Cytometer (Thermo Scientific) and flow cytometry data was analyzed using FlowJo 10.8.1 (Tree Star).
Núñez-Ruiz A., Sánchez-Brena F., López-Pacheco C., Acevedo-Domínguez N.A, & Soldevila G. (2022). Obesity modulates the immune macroenvironment associated with breast cancer development. PLoS ONE, 17(4), e0266827.
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4

Comprehensive Murine and Human Immune Profiling

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Flow cytometry antibodies for murine samples included CD3e-PE, PD1-BV605, CD45-BV786 (BDbiosciences), CD90.1-FITC, CD62L-PECy7, CD45.1-PE (ebioscience), CD45.2-Alexa700, CD-8α-Percp Cy5.5, CD4-FITC, CD103-APC, CD24-APCcy7 (Invitrogen), CD44-APCy7, CD4-BV421, F4/80-PercpCy5.5, CD39-PECy7, PDL1-PE, CD90.2-Alexa 700, MHCII-BV421, CD11b-BV650, Ly6C-BV711 (Biolegend), CD8α-PE-Texas red (life technologies), CD278 (ICOS)-PE (Biolegend). Flow cytometry antibodies for human samples included CD45-BV510, CD3-Alexa700, CD4-BV785, CD8-BV711, CTLA-4-PE/Dazzle 594, 4-1BB-PE (Biolegend), CD39-BV650, PD-1-PE-Cy7 and Ki-67 Alexa 488 (BD Biosciences), CD103-APC and FOXP3-Alexa 700 (eBioscience).
Mouse antibodies for the explant assays included anti-PD1 (RMP1-14) and anti-CTLA-4 (9D9) by BioXCell and OX40 (OX86) kindly provided by Dr. Andrew Weinberg (EACRI). Human antibodies for the explant assays included anti-PD1 and anti-CTLA4 purchased from Invitrogen and anti-OX40 kindly provided by Dr. Andrew Weinberg (EACRI).
Sharon S., Duhen T., Bambina S., Baird J., Leidner R., Bell B., Casap N., Crittenden M., Vasudevan S., Jubran M., Kravchenko-Balasha N, & Gough M. (2021). Explant Modeling of the Immune Environment of Head and Neck Cancer. Frontiers in Oncology, 11, 611365.
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