For transplantation experiments female C57BL/6J mice were obtained from Janvier labs (Le Genest-Saint-Isle, France). For the isolation of MSC, C57BL/6-Tg(CAG-EGFP)1Osb/J (eGFP) were purchased from the Jackson Laboratory (Sacramento, CA) and further bred in the Central Animal Facility (Heinrich-Heine-University, Duesseldorf, Germany). Mice were kept under 12:12 h light:dark cycle with food and water ad libitum. All experiments were implemented in accordance with the “Association for Research in Vision and Ophthalmology” Statement for the use of animals in ophthalmic and vision research, the national ethical committee for animal experimentation (FELASA guidelines) and were approved by the “Landesamt für Natur, Umwelt und Verbraucherschutz Nordrhein-Westfalen” (LANUV, IRB No. 84-02.04.2013.A268).
C57bl 6 tg cag egfp 1osb j egfp
Manufactured by Jackson ImmunoResearch
Sourced in Montenegro, United States
C57BL/6-Tg(CAG-EGFP)1Osb/J (eGFP) is a transgenic mouse strain that expresses the enhanced green fluorescent protein (eGFP) under the control of the CAG promoter. The CAG promoter is a powerful, ubiquitously active promoter, leading to the expression of eGFP in most cell types of the mouse.
Automatically generated - may contain errors
4 protocols using c57bl 6 tg cag egfp 1osb j egfp
1
Isolation and Transplantation of Murine MSCs
2
Mouse Strains and Animal Care Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All mice were maintained under specific pathogen–free conditions in the animal facilities of Sun Yat-sen University Cancer Center (Guangzhou, China). WT C57BL/6 mice (6–8 wk of age, GDMLAC87) were purchased from Guangdong Medical Laboratory Animal Center. Non-obese diabetic/severe combined immunodeficiency mice (6–8 wk of age, T001492) and B6/JGpt-Rosa26em1Cin (CAG-cas9-tdTomato)/Gpt (Cas9-tdTomato, T004285) mice were purchased from GemPharmatech. Tg(CAG-KikGR)33Hadj (KikGR; 013753B6, RRID: IMSR_JAX:013753) mice, SJL-Ptprca Pepcb/BoyJ (CD45.1+; 002014, RRID: IMSR_JAX: 002014) mice, heterozygous C57BL/6J-ApcMin/J (ApcMin/+; 002020, RRID: IMSR_JAX: 002020) mice, and C57BL/6-Tg(CAG-EGFP)1Osb/J (EGFP+; JAX:003291, RRID:IMSR_JAX:003291) mice were purchased from The Jackson Laboratory. The animal experimental protocols were performed according to state guidelines and were approved by the Institutional Animal Care and Use Committee of Sun Yat-sen University.
3
Tracing CD45-Cre Lineage in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Wild‐type (WT) C57BL/6J and C57BL/6‐Tg(CAG‐EGFP)1Osb/J (eGFP) were obtained from The Jackson Laboratory (Bar Harbor, ME), and CD45‐Cre and Ai9(RCL‐tdT) reporter mice were a kind gift from Dr Edwin Horwitz at The Research Institute at Nationwide Children's Hospital (Columbus, OH). Male CD45‐Cre mice were crossed with female Ai9 mice to report Cre recombinase activity in embryonic day 11.5, postnatal day (PND) 2, and 6‐week‐old adult CD45‐Cre+;Ai9+/− progeny. CD45‐Cre−;Ai9+/− littermates were used as controls. All animal procedures were approved and performed in accordance with Institutional Animal Care and Use Committee and institutional guidelines provided by The Research Institute at Nationwide Children's Hospital.
4
Mouse Phenotyping with Fluorescent Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6J (wild type, WT), C57BL/6-Tg(CAG-EGFP)1Osb/J (EGFP), B6.129P2(Cg)-Cx3cr1tm1Litt/J (CX3CR1GFP/+), and B6.Cg-Tg(CAG-mRFP1)1F1Hadj/J (RFP) mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA) and maintained in a pathogen-free animal facility in Florida State University. Animal housing, handling, and all procedures were approved by the Animal Care and Use Committee (ACUC) of Florida State University. All results from animal work were evaluated and quantified using double-blinded methods.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!