Nts cre

Manufactured by Jackson ImmunoResearch

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The Nts-Cre is a recombinant Cre protein produced by Jackson ImmunoResearch. Cre recombinase is an enzyme derived from the P1 bacteriophage that catalyzes site-specific recombination between 34 base pair loxP recognition sites.

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6 protocols using nts cre

Transgenic Mouse Strains for Neuroscience

Cited 2 times

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Wild-type C57BL/6J (000664), Glp1r-ires-Cre (029283), Gpr65-ires-Cre (029282), Npy2r-ires-Cre (029285), P2ry1-ires-Cre (029284), Agtr1a-Cre (030553), Calb2-ires-Cre (010774), Nts-Cre (017525), Vglut2-ires-Cre (016963), Sst-ires-Cre (013044), Twist2-Cre (008712), Vip-ires-Cre (010908), Piezo2-GFP-ires-Cre (027719), Trpv1-Cre (017769), Pvalb-Cre (017320), Vglut1-ires2-Cre (023527), Chat-ires-Cre (031661), lox-ChR2 (024109), lox-tdTomato (007914), and Snap25-2A-GCaMP6s-D (025111) were from the Jackson Laboratory. Drd2-Cre (032108-UCD) mice were from the Mutant Mouse Resource and Research Center (MMRRC). lox-L10-GFP mice were described before^{11 (link),12 (link)}.

Zhao Q., Yu C.D., Wang R., Xu Q.J., Dai Pra R., Zhang L, & Chang R.B. (2022). A multidimensional coding architecture of the vagal interoceptive system. Nature, 603(7903), 878-884.

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Transgenic Mouse Strain Breeding

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All procedures were approved and conducted in accordance with the guidelines of the Institutional Animal Care and Use Committee of the University of Washington. Mice were group-housed on a 12-h light/dark cycle with ad libitum food and water. Approximately equal numbers of male and female mice were used for all experiments. Crh-Cre (Stock number 012704), Nts-Cre (Stock number 017525), Tac2-Cre (Stock number 021878), and Ai14 TdTmto (Stock number 007914) mice are available from Jackson Laboratories.

Soden M.E., Yee J.X., Cuevas B., Rastani A., Elum J, & Zweifel L.S. (2022). Distinct Encoding of Reward and Aversion by Peptidergic BNST Inputs to the VTA. Frontiers in Neural Circuits, 16, 918839.

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Transgenic Mouse Models for Neuroscience

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C57BL/6J (Stock# 000664), Nts-Cre (Leinninger et al., 2011 (link); Stock# 017525), Sst-Cre (Stock# 028864), Cartpt-Cre (Stock# 028533), and Glp1r-cre (Stock# 029283) mice were obtained from Jackson Laboratories and housed according to the guidelines of the Rockefeller’s University Animal Facility under protocol 17105 and protocol 18039. Male and female mice between 8 and 20 weeks of age were used throughout this study and all animal experiments were approved by the Rockefeller University IACUC, according to NIH guidelines. Male and female mice were used for Nts-cre, Sst-cre, Cartpt-cre, and Glp1r-cre experiments. In experiments that used Cre+ and Cre– mice, littermates were used. No differences were determined based on sex and the number of males and females was counterbalanced during experiments. Mice were kept on a 12 hr/12 hr light/dark cycle (lights on at 7:00 a.m.) and had access to food and water ad libitum, except when noted otherwise. Mice were housed together (4–5 mice/cage) for most experiments, except feeding experiments (one mouse/cage). All feeding experiments used standard rodent chow pellets.

Azevedo E.P., Tan B., Pomeranz L.E., Ivan V., Fetcho R., Schneeberger M., Doerig K.R., Liston C., Friedman J.M, & Stern S.A. (2020). A limbic circuit selectively links active escape to food suppression. eLife, 9, e58894.

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Cre-based Mouse Strain Characterization

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Adult LepR-Cre (JAX #032457),⁷³ (link) Nts-Cre (JAX #017525),⁴⁴ (link) and C57BL/6 mice (JAX #000664) were used in this study (The Jackson Laboratory, Bar Harbor, USA). Both Cre lines were backcrossed to the C57BL6/J strain. All experimental procedures were performed in accordance with national and international guidelines (ARRIVE) and approved by the local health authority (LANUV). Animals were housed in groups in the animal facility under standard conditions at a room temperature between 20 and 24°C and on a reversed 12 h light/dark cycle (lights off at 9 a.m.). Animals were provided with standard chow (V1554-703, Ssniff, Soest, Germany) and water ad libitum, unless placed on a restriction schedule. Animals of both sexes were used, and were 12–16 weeks of age at the start of the experiment.

Petzold A., van den Munkhof H.E., Figge-Schlensok R, & Korotkova T. (2023). Complementary lateral hypothalamic populations resist hunger pressure to balance nutritional and social needs. Cell Metabolism, 35(3), 456-471.e6.

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Transgenic Mouse Lines for Neural Tracing

Cited 2 times

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Wild-type C57BL/6J (000664), Glp1r-ires-Cre (029283), Gpr65-ires-Cre (029282), Npy2r-ires-Cre (029285), P2ry1-ires-Cre (029284), Agtr1a-Cre (030553), Calb2-ires-Cre (010774), Nts-Cre (017525), Vglut2-ires-Cre (016963), Sst-ires-Cre (013044), Twist2-Cre (008712), Vip-ires-Cre (010908), Piezo2-GFP-ires-Cre (027719), Trpv1-Cre (017769), Pvalb-Cre (017320), Vglut1-ires2-Cre (023527), Chat-ires-Cre (031661), lox-ChR2 (024109), lox-tdTomato (007914), and Snap25–2A-GCaMP6s-D (025111) were from the Jackson Laboratory. Drd2-Cre (032108-UCD) mice were from the Mutant Mouse Resource and Research Center (MMRRC). lox-L10-GFP mice were described before^{11 (link),12 (link)}.

Zhao Q., Yu C.D., Wang R., Xu Q.J., Dai Pra R., Zhang L, & Chang R.B. (2022). A multidimensional coding architecture of the vagal interoceptive system. Nature, 603(7903), 878-884.

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Genetic mouse models for neuroscience

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Animal experiments were approved by The Rockefeller University Animal Care and Use Committee and were carried out in accordance with the National Institutes of Health guidelines. All experiments were conducted on male mice older than 8 weeks of age housed in a 12-h light/12-h dark cycle at 22 °C and 30–60% humidity. We used the following genotypes of mice: C57BL/6J (wild type; number 000664, The Jackson Laboratory), FOS^2A-iCreER (TRAP2) mice (The Jackson Laboratory, stock number 030323), CAG–Sun1/sfGFP (INTACT) mice (The Jackson Laboratory, stock number 021039), VGLUT2–IRES–Cre mice (The Jackson Laboratory, stock no. 028863), VGAT–IRES–Cre mice (The Jackson Laboratory, stock no. 028862), ADCYAP1–2A–Cre mice (The Jackson Laboratory, stock number 030155), PHOX2B–Cre (The Jackson Laboratory, Stock 016223), DBH–Cre (The Jackson Laboratory, stock number 033951), TH–Cre (The Jackson Laboratory, stock number 008601), TAC1–Cre (The Jackson Laboratory, stock number 021877), PDYN–Cre (The Jackson Laboratory, stock number 027958), CCK–Cre (The Jackson Laboratory, stock number 012706), NTS–Cre (The Jackson Laboratory, stock number 017525). For all Cre mouse line experiments, only heterozygous animals were used. Sample sizes were decided based on experiments from similar studies. Animals were randomly assigned to groups when possible.

Ilanges A., Shiao R., Shaked J., Luo J.D., Yu X, & Friedman J.M. (2022). Brainstem ADCYAP1+ neurons control multiple aspects of sickness behaviour. Nature, 609(7928), 761-771.

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