Hypoxanthine, sorbitol, percoll, MG132, Epoxomicin, Lactacystin, Clasto-Lactacystin β-lactone, Gliotoxin, MG115, EDTA, sodium phosphate dibasic, monosodium phosphate, Tris HCl, sodium chloride, Tween-20, sodium pyrophosphate, glycerol-2-phosphate, saponin, bovine serum albumin (BSA), dithiothreitol (DTT) and phenylmethylsulphonyl fluoride (PMSF) were purchased from Sigma. Glutathione acceptor beads, protein A donor beads, DELFIA enhancement solution and DELFIA secondary antibody (Eu-N1 rabbit Anti-mouse-IgG) came from Perkin Elmer. NP-40 was purchased from Calbiochem, sodium fluoride from Panreac, antibody anti-ubiquitin P4D1 from Santacruz, deubiquitylases inhibitor PR-619 came from Merck, complete mini EDTA protease inhibitor cocktail from Roche, antibody anti-ubiquitin FK2 from Enzo, biotin-TUBEs from Life sensor, RPMI 1640 medium from Gibco, AlbuMAX II from Invitrogen, bortezomib from Selleckchem, enhanced chemiluminescence (ECL) from GE Healthcare and PBS from Oxoid. Atovaquone was prepared in house.
Sodium fluoride
Manufactured by ITW Reagents
Sodium fluoride is a chemical compound with the formula NaF. It is a white crystalline solid that is commonly used in various laboratory applications. Sodium fluoride serves as a source of fluoride ions and can be used in titrations, buffer solutions, and other analytical procedures. Its core function is to provide a controlled source of fluoride ions for chemical analysis and experimentation.
Automatically generated - may contain errors
Lab products found in correlation
Lactacystin, by Merck Group (1 mentions)
Sodium pyrophosphate, by Merck Group (1 mentions)
Monosodium phosphate, by Merck Group (1 mentions)
Gliotoxin, by Merck Group (1 mentions)
Epoxomicin, by Merck Group (1 mentions)
Hypoxanthine, by Merck Group (1 mentions)
Phenylmethylsulphonyl fluoride, by Merck Group (1 mentions)
Enhanced chemiluminescence ecl, by GE Healthcare (1 mentions)
Sorbitol, by Merck Group (1 mentions)
Mg115, by Merck Group (1 mentions)
Glycerol 2 phosphate, by Merck Group (1 mentions)
Clasto lactacystin β lactone, by Merck Group (1 mentions)
Glutathione acceptor beads, by PerkinElmer (1 mentions)
Protein a donor beads, by PerkinElmer (1 mentions)
Complete mini edta protease inhibitor cocktail, by Roche (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Albumax 2, by Thermo Fisher Scientific (1 mentions)
Mg132, by Merck Group (1 mentions)
Percoll, by Merck Group (1 mentions)
Bortezomib, by Selleck Chemicals (1 mentions)
2 protocols using sodium fluoride
1
Comprehensive Ubiquitin Assay Protocol
2
Mitochondria Isolation and Western Blot Analysis
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Mitochondria were isolated using a Mitochondria Isolation Kit (ThermoFisher, 89874); the option A of the manufacturer's instructions was selected. The required amounts of reagent A and reagent B were supplemented with protease inhibitor cocktail 10X (Sigma-Aldrich, P2714), 0.5 M 20% sodium orthovanadate (Sigma-Aldrich, S6508), and 0.1 M 1% sodium fluoride (Panreac, 131675). Isolated mitochondria were lysed in 2% CHAPS (Sigma-Aldrich, C3023). Cytosolic and mitochondrial extractions were analyzed by Western blotting (WB).
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