Active immunizations were performed as we have previously described (42 (link)). Briefly, rIsdB (GenScript) was emulsified with adjuvant (Sigma Adjuvant System, S6322, MilliporeSigma) and i.p. injected 3 times (day –28, –14, and –7) to immunize the mice. Adjuvant was injected as the same manner as a control. Serum antibody titers against the antigen were determined by ELISA.
Risdb
Manufactured by GenScript
The RIsdB is a laboratory equipment product offered by GenScript. It functions as a device for performing Rapid Immunoassay System for Detection of Biomolecules. The core function of the RIsdB is to enable rapid and sensitive detection of various biomolecules, such as proteins, antibodies, or other analytes, through immunoassay techniques. The detailed specifications and intended use of this product are not available for inclusion in this unbiased and factual description.
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Lab products found in correlation
2 protocols using risdb
1
Mouse Active Immunization Protocol
2
Immunoprecipitation of IsdB-Hb-Hp Complex
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Anti-IsdB mAb (anti-IsdB 1.5), rIsdB (GenScript), Hb (MilliporeSigma), and Hp (MilliporeSigma) was separated by SDS-PAGE under reducing conditions, followed by Coomassie blue staining to illustrate the purity of the input proteins. For immunoprecipitation, a combination of rIsdB (40 μg/mL), Hb (25 μg/mL), and Hp (35 μg/mL) was incubated at 37°C for 2 hours. They were immunoprecipitated with anti-IsdB mAb (anti-IsdB 1.5, 50 μg/mL) or irrelevant IgG (50 μg/mL, MilliporeSigma) and Spa-coupled beads (Thermo Fisher Scientific). Proteins bound to the beads were analyzed via immunoblotting with anti-Hp mAb (Abcam).
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