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Surviving

Manufactured by Cell Signaling Technology
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Sourced in Germany

Surviving is a laboratory equipment product designed to facilitate survival analysis experiments. It provides core functionality for monitoring and analyzing the survival rates of cell populations or other biological entities under various experimental conditions.

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Lab products found in correlation

Cleaved parp, by Cell Signaling Technology (2 mentions) Livin, by Cell Signaling Technology (1 mentions) Ciap1, by Cell Signaling Technology (1 mentions) Ciap2, by Cell Signaling Technology (1 mentions) Snai2, by Cell Signaling Technology (1 mentions) Cytokeratin 7, by Abcam (1 mentions) T smad2 3, by Cell Signaling Technology (1 mentions) P smad2, by Cell Signaling Technology (1 mentions) Gapdh, by Merck Group (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions) Anti mouse and anti rabbit igg, by Cytiva (1 mentions) Bcl xl, by Cell Signaling Technology (1 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions) Bcl 2, by Cell Signaling Technology (1 mentions)

2 protocols using surviving

1

Comprehensive Western Blot Analysis

Cited 1 time
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Western blot was performed as described previously [24 (link)].The primary antibodies were purchased from Cell signaling including β-catenin(#8480S), snai2(#9585S), vimentin (#5741S), surviving (#2808S), XIAP(#14334S), cIAP1(#7065S), cIAP2(#3130S), livin (#5471S), cleaved-PARP(#5625S), cleaved-caspase3(#9661S), T-SMAD2/3 (#8685S), P-SMAD2 (#18338S). GAPDH was purchased from Sigma (#G9545) and cytokeratin-7 was purchased from Abcam (#ab181598).
Zhao G., Wang Q., Wu Z., Tian X., Yan H., Wang B., Dong P., Watari H., Pfeffer L.M., Guo Y., Li W, & Yue J. (2019). Ovarian primary and metastatic tumors suppressed by survivin knockout or a novel survivin inhibitor. Molecular cancer therapeutics, 18(12), 2233-2245.
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2

D2O-Treated Cell Protein Analysis

Cited 1 time
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Protein extracts of cells treated with D2O for 48 hrs were separated by SDS-polyacrylamide gels and electroblotted as described.12 (link) Membranes were incubated with the following primary antibodies: Bcl-2, Bcl-XL, Bax, AIF, cleaved PARP, surviving (all purchased from Cell Signaling, Frankfurt, Germany) and actin (Heidelberg, Santa Cruz). Primary antibody application was followed by incubation with horseradish peroxidase-conjugated secondary antibodies (anti-mouse and anti-rabbit IgG, Amersham, Uppsala, Sweden). Blots were developed using an enhanced chemiluminescence detection system (ECL) (Amersham) according to the manufacturer’s instructions.
Kleemann J., Reichenbach G., Zöller N., Jäger M., Kaufmann R., Meissner M, & Kippenberger S. (2020). Heavy Water Affects Vital Parameters of Human Melanoma Cells in vitro. Cancer Management and Research, 12, 1199-1209.
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