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2 protocols using dodecylphosphocholine d38

1

Peptide and Lipid Synthesis for Membrane Research

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GW-Q6 peptide and biotinylated GW-Q6 (GIKIAKKAITIAKKIAKIYW) were synthesized by Kelowna International Scientific Inc., Taipei, Taiwan, with more than 95% purity and their molecular sizes were verified by mass spectrum analysis. 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG) were purchased from Avanti Polar Lipids, Inc. Calcein, MnCl2, 5-, 12-, 16-doxyl stearic acids, and 8-anilino-1-naphthalenesulfonic acid (ANS) were purchased from Sigma-Aldrich Inc. Dodecylphosphocholine-d38, methanol-d4 and D2O were supplied from Cambridge Isotope Laboratories, Inc. Sodium dodecyl sulfate was obtained from Merk (Darmstadt, Germany). Streptavidin gel was purchased from GE Healthcare (Uppsala, Sweden). Sodium N-dodecanoylsarcosinate (sarkosyl) was supplied by Wako Pure Chem (Osaka, Japan). 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) was purchased from ThermoFisher (St. Waltham, MA, USA).
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2

TP4 Peptide Synthesis and Characterization

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TP4 (FIHHIIGGLFSAGKAIHRLIRRRRR) and its derived peptides were synthesized by Kelowna International Scientific Inc. (Taipei, Taiwan) with more than 95% purity and their molecular weights were verified by mass spectrum analysis. Sodium dodecyl sulfate (SDS) was obtained from Merck (Darmstadt, Germany). Streptavidin gel was purchased from GE Healthcare (Uppsala, Sweden). Sodium N-dodecanoylsarcosinate (sarkosyl) was supplied by Wako Pure Chem. (Osaka, Japan). 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) was purchased from Thermo Fisher (St. Waltham, MA, USA). 3,3’-dipropylthiadicarbocyanine iodide (DiSC3(5)) was obtained from Molecular Probes (OR, USA). Dodecylphosphocholine (DPC) was purchased from Avanti Polar Lipids, Inc. Crystal violet, MnCl2 and 8-anilino-1-naphthalenesulfonic acid (ANS) were purchased from Sigma-Aldrich Inc. Dodecylphosphocholine-d38 (DPC) and D2O were supplied by Cambridge Isotope Laboratories, Inc.
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