Freshly isolated BM cell populations (1 × 105/sample) were washed with 2% FCS/PBS and incubated with antibodies against CD105 (clone MJ7/18, PE-labeled; BioLegend, UK), CD33D1 (clone 33D1; APC-labeled; Biolegend, UK), CD29 (clone HMβ-1; FITC-labeled; Biolegend), FITC-labeled anti-mouse F4/80 antibodies (clone Ba18, Biolegend, USA), FITC-labeled anti-mouse CD3e antibodies (clone 145-2611, Biolegend, UK), FITC-labeled anti-mouse CD11c (clone N418, Biolegend, UK), and IgG isotype controls for 15 min at 4°C. After washing with 2% FCS/PBS the samples were analyzed by flow cytometry. CD14 and CD19 populations were retained by MACS using CD14 or CD19 microbeads (Miltenyi Biotec, Germany). Cells were washed and incubated with antibody against RANK (CD265, clone 6D5; FITC-labeled; Biolegend, USA) for FACS analysis (FACSDiva v6.1.2 Software, Becton Dickinson GmbH, San Jose, CA, USA.
Fitc labeled anti mouse cd11c clone n418
Manufactured by BioLegend
Sourced in United Kingdom, United States
FITC-labeled anti-mouse CD11c (clone N418) is a flow cytometry antibody used for the detection of CD11c, a surface marker expressed on dendritic cells. This product provides a direct method for identifying and enumerating CD11c-positive cells in mouse samples.
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Lab products found in correlation
2 protocols using fitc labeled anti mouse cd11c clone n418
1
Bone Marrow Cell Immunophenotyping
2
Comprehensive Immunophenotyping of Dendritic Cells
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Flow cytometric analyses were performed to determine the surface molecule expression of the cells using a FACSCanto II six-color cytometer (Becton Dickinson Immunochemical Systems, Mountain View, Calif., USA). Half a million cells were pelleted and resuspended in 100 µl PBS containing 1% FCS and 0.1% sodium azide. Fluorescent dye-labeled mAbs were added to the pellet and incubated for 30 min at 4°C. The cells were then washed twice and resuspended in PBS. The following antibodies were used: FITC-labeled anti-mouse CD11c (clone N418; BioLegend, San Diego, Calif., USA), PE or PE/Cy7-labeled anti-mouse CD205 (DEC-205) (clone NLDC-145; BioLegend), APC-labeled anti-mouse DC marker (33D1) (clone 33D1; BioLegend), PE-labeled anti-mouse CD1d (clone 1B1; BioLegend), APC/Cy7-labeled anti-mouse MHC class II (MHC-II) (clone M5/114.15.2; BioLegend), PE-labeled anti-mouse CD8α (clone 53-6.7; BioLegend), PE-labeled anti-mouse CD80 (clone 16-10A1; BioLegend), PE-labeled anti-mouse CD86 (clone GL1; BioLegend), PE-labeled anti-mouse CD40 (clone 3/23; BioLegend), PE-labeled anti-mouse PD-L1 (clone 10F.9G2; BioLegend), and PE-labeled anti-mouse α-GalCer:CD1d complex (clone L363). Dead cells were gated out by forward and side scatter based on propidium iodide uptake. 10,000 events were acquired for each sample and analyzed using FlowJo software (Tree Star Inc., Ashland, Oreg., USA).
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