Sodium malonate

Manufactured by Merck Group

Sourced in Spain

Sodium malonate is a chemical compound used as a laboratory reagent. It is a white crystalline solid that is soluble in water. Sodium malonate is commonly used as a buffering agent in biochemical and analytical applications.

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Malonate (25 citations) Sodium malonate dibasic (1 citations)

3 protocols using sodium malonate

Dunaliella tertiolecta Inhibition Assay

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Dunaliella tertiolecta UTEX 999 culture was obtained from UTEX. The inhibitors used in this study 3‐(3,4‐dichlorophenyl)‐1,1‐dimethylurea (DCMU; Catalog no: D2425), 2,5‐dibromo‐3‐methyl‐6‐isopropyl‐p‐benzoquinone (DBMIB; Catalog no: 71993), sodium malonate (Catalog no: 63409), 6‐aminonicotinamide (Catalog no: A68203), antimycin A (Catalog no: A8674), and aminooxyacetate (AOA; Catalog no: C13408‐1G) were all purchased from Sigma‐Aldrich. The oil used for preparation was a Macondo surrogate oil provided by BP Oil.

Kamalanathan M., Mapes S., Prouse A., Faulkner P., Klobusnik N.H., Hillhouse J., Hala D, & Quigg A. (2022). Core metabolism plasticity in phytoplankton: Response of Dunaliella tertiolecta to oil exposure. Journal of Phycology, 58(6), 804-814.

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Analytical Procedures for Chemical Extraction

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All solvents used for solid phase extraction and HPLC-DAD analyses were LC-MS grade from either Sigma-Aldrich (Madrid, Spain) or VWR Chemicals (Barcelona, Spain). Restriction enzymes and T4 DNA ligase were purchased from Thermo Scientific (Madrid, Spain). p-Coumaric acid, L-phenylalanine and sodium malonate were purchased from Sigma-Aldrich (Madrid, Spain), L-tyrosine from Acros Organics (Thermo Fisher, Madrid, Spain), and cerulenin from Cayman Chemicals (Ann Arbor, Michigan, USA).

Ye S., Magadán-Corpas P., Pérez-Valero Á., Villar C.J, & Lombó F. (2023). Metabolic engineering strategies for naringenin production enhancement in Streptomyces albidoflavus J1074. Microbial Cell Factories, 22, 167.

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Acetyl-CoA and Oxaloacetate Enzymatic Assay

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Acetyl‐CoA (≥93% purity), adenosine diphosphate (ADP; 97%), ATP (≥99%), sodium malonate (≥99%) and tris(2‐carboxyethyl)phosphine hydrochloride solution (pH = 7.0, TCEP) were purchased from Sigma; malonyl‐CoA (≥90%) and CoA (≥95%), from Cayman. Oxaloacetatic acid (98%) was purchased from Acros, cyclic AMP (cAMP; ≥ 98%) and AMP (99.5%) from VWR international GmbH. Oxaloacetate stock solution was prepared in buffer C and adjusted to pH 8.0. All stock solutions were stored at −80°C until use.

Maehler D., Hoefgen S., Münchberg U., Schmitz O.J., Rautschek J., Huang Y., Freier E, & Valiante V. (2022). Time‐resolved multiparameter analytics on a cell‐free production platform for acyl‐CoA precursors. Analytical Science Advances, 3(11-12), 289-296.

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