Connective tissue growth factor

Manufactured by Abcam

Sourced in United Kingdom

Connective Tissue Growth Factor (CTGF) is a protein involved in the regulation of various cellular processes, including cell proliferation, differentiation, and extracellular matrix production. It plays a role in the development and maintenance of connective tissues.

Automatically generated - may contain errors

Lab products found in correlation

Amersham ecl, by GE Healthcare (1 mentions) Sds sample buffer, by Thermo Fisher Scientific (1 mentions) Endothelin 1, by Abcam (1 mentions) Tcs sp2, by Leica (1 mentions) Eclipse e800 microscope, by Nikon (1 mentions) Tcs sp5 microscope, by Leica (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) Laminin, by Agilent Technologies (1 mentions) Aldh1a1, by Abcam (1 mentions)

2 protocols using connective tissue growth factor

Western Blot Analysis of Cardiac Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cardiac tissues samples were homogenized with lysis buffer and diluted 1:1 with 2 × SDS sample buffer (Invitrogen Novex, Carlsbad, CA). An equal amount of protein (30 μg) was loaded onto each lane of an 8% to 16% Tris‐Glycin gel (Helixx). Proteins were separated by electrophoresis and transferred to a nitrocellulose membrane using an electro‐blotting apparatus (Invitrogen, Carlsbad, CA). Membranes were incubated with 5% Bovine Serum Albumin (BSA) for 1 h to decrease nonspecific binding. Samples were then incubated with the following primary antibodies overnight at 4°C: Connective Tissue Growth Factor (CTGF) (Abcam, Cambridge, UK), SMAD3 and 4 (R&D Systems Inc. Minneapolis, MN), pSMAD3 (Cell Signaling Techonology Inc. Danvers, MA) Endothelin‐1 (Abcam, Cambridge, UK), and matrix metalloproteinases 2 (MMP‐2) (EMD Millipore, Billerica, MA). Caspase 3 and 8 (Cell Signaling Techonology Inc. Danvers, MA), α and β MHC (EMD Millipore, Billerica, MA) samples were washed and incubated with peroxidase – conjugated secondary antibody, and detected using the Amersham ECL (GE life sciences, Buckinghamshire, UK) detection kit. Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) was used as the internal standard.

Nielsen E.A., Okumura K., Sun M., Hjortdal V.E., Redington A.N, & Friedberg M.K. (2017). Regional septal hinge‐point injury contributes to adverse biventricular interactions in pulmonary hypertension. Physiological Reports, 5(14), e13332.

+ Open protocol

+ Expand

Endothelial Components Immunostaining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

To examine for specific endothelial components, samples were incubated with primary antibodies against Laminin (cat# Z0097: DAKO; Carpinteria, CA), Aldehyde dehydrogenase 1 family, member A1 (ALDH1A1, cat# ab23375: Abcam; Cambridge, MA), Connective Tissue Growth Factor (CTGF, cat# ab59681: Abcam), Zonula occludens-1 (ZO-1, cat# 339100: Life Technologies; Grand Island, NY), Caspase-3 (cat# 9661: Cell Signaling; Danvers, MA), and Ki67 (cat# VP-K452: Vector Laboratories Inc.; Burlingame, CA), and then incubated with corresponding secondary antibody (Jackson ImmunoResearch; West Grove, PA), as previously described (Karamichos et al., 2010 (link)). TOPRO-3 iodide (642/661, cat# T3605: Thermo Fisher Scientific; Waltham, MA) or DAPI (cat# H-1200: Vector Laboratories) were used as counterstains to mark all cell nuclei. Whole mount samples were observed and imaged using either a confocal TCS-SP2 or TCS-SP5 Leica microscope (Leica Microsystems; Bannockburn, IL). Frozen sections and 2D cultures were observed with a Nikon Eclipse E800 microscope equipped with an Andor Clara E camera and Nikon NIS Elements for Basic Research (Micro Video Instruments; Avon, MA). Negative controls, where primary antibody was omitted, were performed with every experiment, and each experiment was performed at least three times.

Hutcheon A.E., Zieske J.D, & Guo X. (2019). 3D In Vitro Model for Human Corneal Endothelial Cell Maturation. Experimental eye research, 184, 183-191.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!