Anti pd l2

For FC staining, non-specific binding was blocked using anti-FcR clone 2.4G2 and dead cells were excluded using cell viability dye (Tonbo Biosciences). The Abs were either purified in our lab or purchased and are as follows. Anti-B220 (Biolegend, RA3-6B2), anti-CD19 (BD ID3), anti-IgM (homemade, B7-6), anti-CD45 (home-made 30-F11), anti-CD21 (homemade, 7G6), anti-CD23 (ebioscience, B3B4) for B cells, anti-CD4 (Biolegend, GK1.5), anti-TCR-β (Biolegend H57-597) for T cells, anti-CD138 (Biolegend, 281-2) and anti-CD44 (Biolegend, IM7) for B cell blasts and PB, anti-CD73 (Biolegend, TY-11.8), anti-CD80 (ebioscience, 16-1oA1), anti-PD-L2 (ebioscience, TY25) for MBC, PNA (vector labs), anti-CD95 (BD, Jo2) for GCBC, anti-CD169 (Biolegend, 3D6.112) for metalophillic macrophages, anti-CD11b (Biolegend M1-70), anti-CD11c (ebioscience, N418), anti-CD69 (ebioscience, H1.2F3), anti-AID (ebioscience, mAID-2) and anti-T-bet (Biolegend, 4B10). The click IT Plus Edu kit was purchased from Invitrogen and the staining was done according to the recommended protocol. The cells were analyzed on LSR II or Fortessa instruments (BD) and the data were analyzed on FlowJo software.