The following antibodies were used for western blotting: ATXN2 mAb (1: 4000) (BD Biosciences Inc., 611378), 5TF1–1C2 mAb (1:3000) (Millipore Inc., MAB1574), RGS8 rabbit polyclonal Ab (1:5000) (Novus Biologicals, NBP2–20153), PCP-2 antibody (F-3) (1: 3000) (Santa Cruz Inc., sc-137064), β-Actin mAb HRP conjugated (1:10,000) (Sigma Inc., A3854). Anti-PCP4 antibody (1: 5000) (Abcam, ab197377), Homer-3 antibody (E-6) (1: 2000) (Santa Cruz Inc., sc-376155), CEP76 antibody (1: 5000) (Novus biologicals, NBP1–28749), Anti-FAM107B antibody (1: 5000) (Abcam, ab175148). The secondary antibodies were goat anti-mouse IgG-HRP antibody (1:5000) (Sigma Inc., A2304) and goat anti-rabbit IgG-HRP antibody (1:5000) (Vector laboratories, PI-1000). Anti-ASO antibody (Ionis Pharmaceuticals) and appropriate secondary antibody used for IHC are described in the previous paragraph.
Hrp goat anti rabbit igg antibody
Manufactured by Vector Laboratories
Sourced in United Kingdom
The HRP goat anti-rabbit IgG antibody is a secondary antibody that specifically recognizes and binds to rabbit immunoglobulin G (IgG) antibodies. This antibody is conjugated with horseradish peroxidase (HRP), an enzyme used as a detection label in various immunoassays and immunohistochemical techniques.
Automatically generated - may contain errors
Lab products found in correlation
Atxn2 mab, by BD (2 mentions)
Nbp2 20153, by Santa Cruz Biotechnology (2 mentions)
Nbp1 28749, by Abcam (2 mentions)
Goat anti mouse igg hrp antibody, by Merck Group (2 mentions)
Anti vinculin, by Thermo Fisher Scientific (1 mentions)
Anti chk2 pt68, by Cell Signaling Technology (1 mentions)
Anti pchk1 ser345, by Cell Signaling Technology (1 mentions)
Anti phospho histone h2ax ser139, by Cell Signaling Technology (1 mentions)
A21236, by Thermo Fisher Scientific (1 mentions)
A11031, by Thermo Fisher Scientific (1 mentions)
F1804, by Merck Group (1 mentions)
Pi 2000, by Vector Laboratories (1 mentions)
Peroxidase anti mouse igg h l, by Vector Laboratories (1 mentions)
6 protocols using hrp goat anti rabbit igg antibody
1
Western Blot Antibody Validation Protocol
2
Western Blot Antibody Validation Protocol
3
Western Blot Analysis of DNA Damage Signaling
4
Western Blot and Immunofluorescence Assay Protocol
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For Western blots, the
following primary antibodies were diluted in BSA (5%) in 1× PBS
buffer: monoclonal ANTI-FLAG M2 antibody produced in mouse (1:1000,
F1804, Merck); anti-β-actin antibody, mouse monoclonal (1:2000,
A1978, Merck). HRP goat antirabbit IgG antibody (peroxidase) (1:3000,
PI-1000, Vectorlabs), and peroxidase antimouse IgG (H+L) (affinity
purified) (1:3000, PI-2000, Vectorlabs) were used as secondary antibodies.
For IF assays, the following primary antibodies were diluted in
blocking buffer (FBS 3% in 1× PBS): the 5-hydroxymethylcytosine
(5hmC) antibody (pAb) (1:500, Active Motif cat#: 39769)
and monoclonal ANTI-FLAG M2 antibody produced in mouse (1:500, F1804,
Merck). Goat antimouse Alexa Fluor 568 (1:500, A-11031, Invitrogen)
and Goat antirabbit Alexa Fluor 647 (1:500, A-21236, Invitrogen) were
used as secondary antibodies.
following primary antibodies were diluted in BSA (5%) in 1× PBS
buffer: monoclonal ANTI-FLAG M2 antibody produced in mouse (1:1000,
F1804, Merck); anti-β-actin antibody, mouse monoclonal (1:2000,
A1978, Merck). HRP goat antirabbit IgG antibody (peroxidase) (1:3000,
PI-1000, Vectorlabs), and peroxidase antimouse IgG (H+L) (affinity
purified) (1:3000, PI-2000, Vectorlabs) were used as secondary antibodies.
For IF assays, the following primary antibodies were diluted in
blocking buffer (FBS 3% in 1× PBS): the 5-hydroxymethylcytosine
(5hmC) antibody (pAb) (1:500, Active Motif cat#: 39769)
and monoclonal ANTI-FLAG M2 antibody produced in mouse (1:500, F1804,
Merck). Goat antimouse Alexa Fluor 568 (1:500, A-11031, Invitrogen)
and Goat antirabbit Alexa Fluor 647 (1:500, A-21236, Invitrogen) were
used as secondary antibodies.
5
Immunoblotting of TBEV Proteins
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Isolation of proteins was performed from the samples used for RNA isolation using RNA Blue according to the manufacturer’s instructions. The resulting protein isolates were separated on 12 % polyacrylamide gels and subsequent immunoblotting detection was performed as described previously [56] . The following antibodies were used: guinea-pig polyclonal serum against TBEV capsid protein (C) (produced in-house), anti-GAPDH antibody [EPR16891] (Abcam; #ab181602), HRP goat anti-guinea pig (Novex; #A18769), HRP goat anti-rabbit IgG antibody (Vector Laboratories; #PI-1000). Sample inputs were standardised by equal protein amounts loaded into each well (10 µg).
6
Western Blot Analysis of Drosophila Phospho-p70 S6 Kinase
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Protein extracts were prepared from flies homogenized in 0.5 ml of PBS containing 1.5% Triton X-100 and Complete mini EDTA-free Protease Inhibitor Cocktail (Sigma, Missouri, USA). 50μg of protein was mixed with 4X SDS PAGE loading buffer and denatured at 95ºC. Protein mixtures were loaded in Bio-Rad precast gels (Bio-Rad, California, USA) and run at 130 V. Separated proteins were transferred to a nitrocellulose membrane at 30V. Membranes were blocked in 5% BSA in PBS-Tween 1X. Antibodies used were anti-phospho-Drosophila p70 S6 Kinase (Thr398) (Cell Signalling, Massachusetts, USA), anti-p70 S6 kinase alpha (C-18) (Santa Cruz, USA), anti-beta Tubulin [EPR16774] (Abcam, Cambridge, UK), HRP goat anti-rabbit IgG antibody (Vector Laboratories, Peterborough, UK). ImageJ was used for quantification of protein band intensities.
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