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Hrp conjugated anti rabbit or anti mouse igg

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HRP)-conjugated anti-rabbit or anti-mouse IgG is a laboratory reagent used for detection and quantification purposes in various immunological techniques. It consists of horseradish peroxidase (HRP) enzyme conjugated to antibodies that specifically bind to rabbit or mouse immunoglobulin G (IgG).

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Lab products found in correlation

Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (3 mentions) Immobilon p pvdf membrane, by Merck Group (2 mentions) Phosstop phosphotase inhibitor cocktail tablet, by Roche (2 mentions) Antibodies for p akt and hsp90α β, by Santa Cruz Biotechnology (2 mentions) Antibody for gpr120, by Novus Biologicals (2 mentions) Ripa lysis buffer, by Merck Group (2 mentions) P jnk, by Cell Signaling Technology (2 mentions) Protein assay, by Bio-Rad (2 mentions) Complete protease inhibitor cocktail tablet, by Roche (2 mentions) Ab75872, by Abcam (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Ab1930, by Abcam (1 mentions) Anti phosphorylated erk, by Cell Signaling Technology (1 mentions) Cell lysis buffer, by Cell Signaling Technology (1 mentions) Rock1, by Cell Signaling Technology (1 mentions) Mprip, by Proteintech (1 mentions) Eif2α, by Santa Cruz Biotechnology (1 mentions) Anti gfp monoclonal antibody, by BD (1 mentions) Alexa 594, by Thermo Fisher Scientific (1 mentions) Sqstm1 p62, by Abcam (1 mentions)

Close spelling variants of this product

Rabbit anti-HRP HRP anti-mouse Rabbit anti-IgG Rabbit IgGs

7 protocols using hrp conjugated anti rabbit or anti mouse igg

1

Integrin and ERK Protein Analysis

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Anti- integrin αv antibody was obtained from Millipore (AB1930, 1:1000), anti- integrinβ3 antibody from Abcam (ab75872, 1:1000). Anti-phosphorylated ERK was obtained from Cell Signaling Technology (#9101, 1:1000). Immunoblot analysis was performed as described previously10 (link). Tissue or Cells were lysed in cell lysis buffer (Cell Signaling) with protease inhibitor cocktails (Sigma). Lysates were centrifuged at 15,000 g for 15 min at 4 °C, and supernatants were collected. 20–30 μg of cell lysates were separated by SDS-PAGE gel and transferred to Reinforced NC membrane (Whatman GmbH). The membranes were blocked with 5% skim milk in 0.1% Tween 20/TBS buffer and then incubated with primary antibodies. Blots were probed with horse radish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit IgG (Jackson ImmunoResearch). Bands were visualized using Super Signal West Pico Chemiluminescent Substrate (Thermo Scientific).
Chen Z., Ding X., Jin S., Pitt B., Zhang L., Billiar T, & Li Q. (2016). WISP1-αvβ3 integrin signaling positively regulates TLR-triggered inflammation response in sepsis induced lung injury. Scientific Reports, 6, 28841.
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2

Protein Expression Analysis in hESCs

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shNT and shDAP5 hESCs were lysed with RIPA buffer, and 50 µg lysate was resolved on 8.5% SDS-PAGE gels for western blotting, according to standard protocols. The following primary antibodies were used: DAP5 (BD Biosciences, cat# 610742); CCDC88A (Cell Signaling, cat# 14200); MPRIP (Proteintech, cat# 20040-1-AP), ROCK1 (Cell Signaling, cat# 4035T) TOP2B (Proteintech, cat# 20549-1-AP), eIF2α (Santa Cruz, cat# sc11386), phoshoSer51 eIF2α (Abcam, cat# ab32157), Tubulin (Sigma-Aldrich, cat# T9026). Secondary antibodies were HRP-conjugated anti-mouse or anti-rabbit IgG (Jackson ImmunoResearch cat# 111-035-06 or cat# 115-035-003).
David M., Olender T., Mizrahi O., Weingarten-Gabbay S., Friedlander G., Meril S., Goldberg N., Savidor A., Levin Y., Salomon V., Stern-Ginossar N., Bialik S, & Kimchi A. (2022). DAP5 drives translation of specific mRNA targets with upstream ORFs in human embryonic stem cells. RNA, 28(10), 1325-1336.
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3

Detecting Dictyostelium Ras Proteins

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Anti-pan Ras mouse monoclonal antibody from EMD Millipore (Billerica, MA) was used to detect D. discoideum Ras proteins. Anti-GFP monoclonal antibody was from BD Biosciences (San Jose, CA). Anti-GST monoclonal antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). HRP-conjugated anti-mouse or anti-rabbit IgG was obtained from Jackson ImmunoResearch (West Grove, PA). Alexa 594 was from Invitrogen (Carlsbad, CA). pHrodo was from Thermo Fisher Scientific (Waltham, MA).
Xu X., Pots H., Gilsbach B.K., Parsons D., Veltman D.M., Ramachandra S.G., Li H., Kortholt A, & Jin T. (2022). C2GAP2 is a common regulator of Ras signaling for chemotaxis, phagocytosis, and macropinocytosis. Frontiers in Immunology, 13, 1075386.
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4

Western Blot Analysis of Signaling Proteins

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Cells and tissues were washed with cold PBS on ice and treated with RIPA lysis buffer (Millipore, Temecular, CA) containing a cOmplete protease inhibitor cocktail tablet (Roche, Madison, MI) and a PhosSTOP phosphotase inhibitor cocktail tablet (Roche, Madison, MI). The protein amount was determined using the Bio-Rad protein assay (Bio-Rad). After an equal amount of protein was loaded in each lane, they were separated by 10% (w/v) SDS-PAGE and then transferred to an Immobilon-P PVDF membrane (Millipore). Target proteins were immunodetected using specific primary antibodies and the horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG (Jackson ImmunoResearch Laboratories, West Grove, CA) was used as a secondary antibody. Positive bands were detected using the Supersignal Westpico Chemiluminescent Substrate (Thermo Scientific, Pittsburgh, PA). Antibodies for p-TAK1, TAK, p-IKK α/β, IkB-α, p-JNK, JNK and AKT were obtained from Cell Signaling Technology (Boston, MA), antibodies for p-AKT and HSP90α/β were from Santa Cruz Biotechnology (Santa Cruz, CA) and the antibody for GPR120 was from Novus Biologicals (Littleton, CO).
Chung H., Lee Y.S., Mayoral R., Oh D.Y., Siu J.T., Webster N.J., Sears D.D., Olefsky J.M, & Ellies L.G. (2014). Omega-3 fatty acids reduce obesity-induced tumor progression independent of GPR120 in a mouse model of postmenopausal breast cancer. Oncogene, 34(27), 3504-3513.
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5

Western Blot Analysis of Autophagy Markers

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MC3T3‐E1 cells were treated, collected, and lysed with RIPA buffer supplemented with complete protease inhibitor cocktail tablets. The protein concentration was measured using a DC Protein Assay Kit (Bio–Rad Laboratories). Equal amounts of protein were separated on SDS‐polyacrylamide gels and transferred onto PVDF membranes (Millipore). The membranes were then incubated overnight at 4°C with SGK1 (D27C11) (catalog #12103), phospho‐SGK1 (Ser78) (D36D11) (catalog #5599), FoxO3a (75D8) (catalog #2497), Beclin‐1 (D40C5) rabbit monoclonal antibody (catalog #3495), Phospho‐FoxO3a (Ser253) rabbit polyclonal antibody (catalog #9466) (1:1000; Cell Signaling), a LC3 rabbit polyclonal antibody (catalog #NB100‐2220) (1:500; Novus), a SQSTM1/p62 (catalog #ab56416) (1:1000; Abcam), and a β‐actin (clone AC‐15) monoclonal antibody (catalog #ab56416) (1:5000; Sigma‐Aldrich). Next, the membranes were blotted with HRP‐conjugated anti‐rabbit or anti‐mouse IgG (1:5000, Jackson). The protein bands were visualized using Pierce™ ECL Plus Western blot analysis substrate (catalog #32132) according to the manufacturer's instructions and developed with film. The relative density was quantified using ImageJ software (Java; NIH) (Schneider et al., 2012 (link)).
Yu X., Xu X, & Zhang S. (2022). Low‐dose dexamethasone promotes osteoblast viability by activating autophagy via the SGK1/FOXO3a signaling pathway. Cell Biology International, 47(3), 669-678.
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6

Western Blot Analysis of Signaling Proteins

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Cells and tissues were washed with cold PBS on ice and treated with RIPA lysis buffer (Millipore, Temecular, CA) containing a cOmplete protease inhibitor cocktail tablet (Roche, Madison, MI) and a PhosSTOP phosphotase inhibitor cocktail tablet (Roche, Madison, MI). The protein amount was determined using the Bio-Rad protein assay (Bio-Rad). After an equal amount of protein was loaded in each lane, they were separated by 10% (w/v) SDS-PAGE and then transferred to an Immobilon-P PVDF membrane (Millipore). Target proteins were immunodetected using specific primary antibodies and the horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG (Jackson ImmunoResearch Laboratories, West Grove, CA) was used as a secondary antibody. Positive bands were detected using the Supersignal Westpico Chemiluminescent Substrate (Thermo Scientific, Pittsburgh, PA). Antibodies for p-TAK1, TAK, p-IKK α/β, IkB-α, p-JNK, JNK and AKT were obtained from Cell Signaling Technology (Boston, MA), antibodies for p-AKT and HSP90α/β were from Santa Cruz Biotechnology (Santa Cruz, CA) and the antibody for GPR120 was from Novus Biologicals (Littleton, CO).
Chung H., Lee Y.S., Mayoral R., Oh D.Y., Siu J.T., Webster N.J., Sears D.D., Olefsky J.M, & Ellies L.G. (2014). Omega-3 fatty acids reduce obesity-induced tumor progression independent of GPR120 in a mouse model of postmenopausal breast cancer. Oncogene, 34(27), 3504-3513.
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7

Western Blot Analysis of PTBP1 Protein

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Whole-cell lysates were obtained through direct lysis of cells in 50 mM Tris-HCl buffer (pH 8.0) containing 150 mM NaCl, 1% NP40, 1% Triton X-100, 0.1% SDS, 0.5% sodium deoxycholate, and protease inhibitor cocktail (Roche). Equal amounts of these lysates (10 μg per lane) were used for SDS-PAGE and western blot analysis. Samples were immunoblotted against PTBP1 (Rabbit; 1:1,000; ABclonal) or the loading control ACTB (Mouse; 1:20,000; Proteintech), followed by their corresponding HRP-conjugated secondary antibodies (HRP conjugated anti rabbit or anti mouse IgG 1:10,000, Jackson Laboratory). Blots were developed with Immobilon Western Chemiluminescent HRP substrate (Millipore) and visualized using ChemiDoc imaging system (BioRad).
Wang L.L., Serrano C., Zhong X., Ma S., Zou Y, & Zhang C.L. (2021). Revisiting astrocyte to neuron conversion with lineage tracing in vivo. Cell, 184(21), 5465-5481.e16.
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